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Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for immunoblotting: The HA epitope was recognised with a primary mouse monoclonal anti-HA (1:1000; Sigma); the Myc epitope was recognised with a primary mouse monoclonal anti-Myc (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 mg protein lysate in 750 µl SLB buffer with protease and phosphatase inhibitors was incubated with 2.5-5 µg of anti-Flag antibody (Sigma, # F3165) for 2 hours at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4% Triton and the rabbit anti-calbindin-D-28K primary antibody (C7354; Sigma-Aldrich St. Louis, MO, USA; 1:1000). Sections were subsequently washed in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% milk in TBS-T (TBS with 1% Tween-20) and incubated over night at 4°C with the primary anti-FLAG antibody (Sigma F1804-200UG ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies used were as follows: mouse anti-phospho-Histone H2A.X (Ser139; clone JBW301; Sigma, 05-636; 1/1,000), mouse tubulin (clone B-5-1-2 ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted 1:1000 in casein and incubated overnight at 4 degrees Celsius with shaking OR Monoclonal Anti-Beta-Actin mouse antibody (Sigma) diluted 1:5000 in casein and incubated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were afterwards incubated with primary antibodies overnight hours at 4 °C at the following dilutions: anti-tubulin β3 (1:1000; Sigma), anti-GFAP (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... the acini were incubated with the primary antibody mouse anti-podocalyxin (EMD Millipore, clone 3F2:D8, working dilution 1:200), mouse β1 integrin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The elution was separated by 8% Nu-PAGE and analyzed by CBB staining or immunoblotting using an anti-Flag antibody (Sigma).
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
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bioRxiv - Genetics 2022Quote: ... Binding of the anti-FLAG M2 primary antibody (mouse, Sigma-Aldrich, F3165, diluted 1:3000 in PBS-T+1%BSA) was allowed in a humidity chamber (overnight ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated with PBS + 1% milk solution containing a 1:10.000 anti-rabbit horseradish peroxidase conjugated secondary antibody (A0545, Sigma-Aldrich). The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated for 5 min with Pierce ECL Plus substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Microbiology 2022Quote: ... The wells were then washed with ice-cold PBST and incubated with HRP-linked goat anti-chicken antibody (Sigma-Aldrich) for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... SY0917 (MA11010) SABC-Serum) (rabbit, 1:10,000) and a secondary anti-rabbit-HRP (goat) conjugate antibody (1:10,000, Sigma-Aldrich, #31460). The rest of the samples was prepared for mass spectrometric analysis as previously described (Hör et al. ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by incubation in a 1:10000 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25°C ...
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bioRxiv - Plant Biology 2022Quote: ... SDS-PAGE/Western blot analysis was used to identify proteins in the sample with use of anti-FLAG M2 antibody (Sigma) and anti-MYC antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... probed with a 1:10,000 dilution of an anti-guinea pig-horseradish peroxidase-conjugated secondary antibody (Sigma; product no. A5545) for 30 min at ambient temperature ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the samples were incubated with primary antibodies (anti-β-tubulin III produced in rabbit (Sigma, Ireland, T2200, 1:400) and anti-GFAP produced in mouse (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 minutes and then incubated for 48 hours at room temperature in goat anti-ChAT primary antibody (1:1000, AB144P, Millipore). Following rinse ...
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bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Washing steps were performed using PBS-Tween buffer followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody (Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by probing with respective goat anti-mouse or rabbit secondary antibodies conjugated with alkaline phosphatase (dilution 1: 5,000; Sigma-Aldrich) or conjugated with HRP (dilution 1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... α-tubulin antibody was used as loading control (mouse anti-α-tubulin, 1:10000; Sigma-Aldrich cat#T6074, RRID: AB_477582). Antibodies were functionally validated by Cell Signalling Technology and Sigma-Aldrich companies ...
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bioRxiv - Molecular Biology 2022Quote: ... HRPA-1::AID*::TEV::FLAG was detected using Monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody (A8592-2MG) from Sigma-Aldrich at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were separated by 10 % SDS-PAGE gels and detected by immunoblotting with the first anti-GFP or HA-tag antibodies (Sigma) and the second anti-rabbit IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% triton-X 100 in PBS at room temperature for one hour and incubated overnight in 5% FBS and 0.1% triton-X 100 in PBS at 4°C with the following antibodies: anti-LIMCH1 (1:200, catalog number HPA063840, Sigma), anti-mLIMCH1 (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... for one hour and incubated overnight at 4°C in 5% milk/PBS-T with the following antibodies: anti-LIMCH1 (1:400, catalog number HPA063840, Sigma), anti-MBNL1 (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for all stainings were as follows: rabbit anti-TGFA (HPA042297, Sigma-Aldrich, dilution 1:50 and 1:100), rabbit polyclonal anti-pEGFR clone Tyr845 (07-820 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 min at RT and incubated in primary antibody mouse anti-laminA (Sigma Aldrich) at 1:500 overnight at 4 °C ...