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Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for Anti CD152 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 5% skim milk and incubated for 1 hour with primary antibodies diluted in PBS-Tween (1:1,000 monoclonal anti-Flag M2, F3165, Sigma-Aldrich; 1:1,000 monoclonal anti-GFP antibodies (B-2) ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were blocked with 3% BSA in PBS with 0.3% Triton X-100 for 1h at room temperature and subsequently incubated with primary antibodies (rabbit anti-glutamate, 1:500, Sigma; rabbit anti-SOM ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Molecular Biology 2023Quote: ... The process was the same as in fibroblasts and the primary antibodies were anti-human NANOG (1:100, Millipore #AB5731) and SSEA4 (1:25 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated 36 hours at 4°C with the primary antibody – anti-γH2AX (Ser139) clone JBW301 (1:500, 05-636-I, Millipore) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and stained with anti-rabbit IgG antibody conjugated to Alexa488 (Sigma- Aldrich, SAB4600045) and anti-mouse IgG antibody conjugated to Alexa647 (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated overnight at 4 °C with a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a mouse monoclonal anti-poly(GA ...
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bioRxiv - Neuroscience 2023Quote: ... Then the slides were incubated in the above blocking buffer overnight at room temperature with gentle rocking with the primary antibodies as indicated guinea pig anti-ASIC3 (1:2000, Millipore), rabbit anti-TRPV1 (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant solution was removed and the cells were treated with a secondary antibody (the FITC-conjugated anti-mouse IgG (Sigma)) for one hour at 37°C in the darkness ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore; anti-D1R ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was washed 3X with 1x TBST for 5 min each followed by incubation with anti-rat IgG secondary antibody conjugated with horseradish peroxidase (HRP) (Sigma) (1:2000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Polymerized microtubules were diluted in the taxol-BRB80 buffer and then flowed into a flow cell and incubated for 10 min at room temperature to adsorb onto the coverslip surface coated with anti-tubulin antibodies (Sigma). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... S4 a similar protocol was used but the primary antibody mix consisted of mouse anti-GFP 1:1000 (Sigma G6539), rabbit anti-RFP 1:5000 (Rockland 600-401-379) ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed six times with TBST buffer and blotted with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (Sigma). After washing with TBST buffer again ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 10% skim milk (in 1x TBS-T) for 1 h and probed with anti-GFP antibody (1:1,000; Sigma-Aldrich) in 5 % skim milk (in 1x TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were rehydrated in phosphate buffered saline (PBS) and incubated overnight with anti-NaV1.7 antibody (1/200; Cat #: MABN41, Sigma-Aldrich) in PBS with 3% bovine serum albumin at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length FN and 45 kDa-FN GBD were detected with monoclonal mouse anti-FN antibody with epitope within GBD (MAB1892, Sigma), clone IST-10 (1:1000 in 2% BSA in PBS ...
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bioRxiv - Genetics 2023Quote: ... Then cells were resuspended in the BD-FACS staining buffer and labelled through anti-human TRA-1-60 antibody (Millipore) and anti-mouse IgM control ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibodies used for all western blot (WB) experiments and their dilutions were as follows: Anti-FLAG (Sigma, #F3165) at 1:5000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in PBS and incubated with rabbit anti-TH primary antibody (1:200, Millipore AB152, RRID: AB_390204) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... and incubated for 4 hours with an anti-rabbit secondary antibody coupled with Rhodamine (#SAB3700846 Sigma-Aldrich, dilution 1:200). Cells were incubated in AlexaFluor 488 phalloidin (#R37110 ...
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bioRxiv - Biochemistry 2023Quote: ... the plates were incubated with 100 μL/well horseradish peroxidase-conjugated anti-mouse immunoglobulin second antibody (Sigma, St. Louis, MO) in Buffer D of pCa 9 or 4 at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Dig epitopes were detected with alkaline phosphatase-coupled anti-Dig fab fragments antibody at 1:2500 (Millipore Sigma, cat. #11093274910), and the signal revealed with Nitro Blue Tetrazolium chloride/5-Bromo-4-Chloro-3-Indolyl Phosphate ...
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bioRxiv - Genetics 2023Quote: ... Then the cells were incubated for 1 hour at room temperature with 1:500 diluted anti-Cas9 primary antibody (Sigma, SAB4200701-25UL ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
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bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at room temperature (RT) and incubated with mouse monoclonal anti-aggrecan neoepitope antibody (10 µg/ml; clone AF-28, MAB19310-C, Millipore) and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-HA antibody (Sigma) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used were: a rabbit polyclonal anti-GFP in a 1:400 dilution (Millipore Sigma, AB3080, lot: 3717722), a mouse monoclonal anti-NeuN in a 1:500 dilution that is a marker for mature neurons (Millipore Sigma ...
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bioRxiv - Immunology 2023Quote: ... were isolated from PBMCs using anti-CD14-antibody-coated magnetic beads (Miltenyi) and cultured in RPMI1640 supplemented with 10% human AB serum (Sigma), pen/strep ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were washed again and anti-FLAG M2 antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk/0.1% PBS-T) was added and incubated for 1hr at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Flag-ELF7 and GFP-VIP3 ChIP-seqs were performed using 7-day-old seedlings grown under standard conditions and an anti-Flag M2 antibody (F1804, Sigma) and an anti-GFP antibody (ab290 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of the crosslinked UNK-RNA or PABPC1-RNA complexes was carried out using anti-Flag antibody (Millipore Sigma, F1804) or anti-PABPC1 antibody (Abcam ...
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bioRxiv - Genomics 2023Quote: ... then stained with anti-NeuN antibody (directly conjugated to Alexa Fluor 488; Millipore cat. No. MAB377X, clone A60; 1:1,250) and an anti-rabbit IgG Alexa Fluor 647 antibody as a negative control for 30min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... CRHR2α expression levels in the stably transfected cell lines was assessed by Western blot using a mouse monoclonal anti FLAG -antibody (1:5000 dilution; SIGMA). All the experiments were performed at least 3 times with 3 individual clones (clones 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The presence of purified Dr20/22 was confirmed by SDS-PAGE and Western blotting using the Anti-polyHistidine Peroxidase antibody (Sigma). The occurrence of potential glycosylation sites was assessed using the Glycoprotein Staining Kit (Pierce).
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 1 h before immunoreaction with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-GFAP (1:1,000 ...