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Citations for Millipore Sigma :
7101 - 7150 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... One half was used for protein extraction and the other half used for UA quantification using a UA Assay Kit (MAK077, Sigma-Aldrich). Cells were disrupted mechanically ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Cancer Biology 2022Quote: Polyacrylamide gel slices containing bEV and sEV protein lysates were resolved in tris (2-carboxyethyl) phosphine (10 × 10-1 M; Sigma- Aldrich) with ammonium bicarbonate (50 × 10-1 M ...
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bioRxiv - Microbiology 2023Quote: ... and the amount of total protein in each sample was calculated using a 1 mg/ml standard sample of bovine serum albumin (Sigma-Aldrich). To separate the soluble and aggregated proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was filtered with a 0.22 µm filter and concentrated using Amicon Ultra-15 centrifugal filter units (Millipore Sigma Cat#UFC901008). Protein was then loaded onto a Superdex 200 column and 5 mL fractions were collected ...
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bioRxiv - Cancer Biology 2023Quote: ... 80 µg of equivalent proteins from sample were treated with 5mM tris (2-carboxyethyl) phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) for 15 minutes at 37°C to reduce the disulfide bonds ...
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bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
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bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Immobilion-P, Millipore, Schwalbach, Germany) by semi-dry blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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bioRxiv - Molecular Biology 2023Quote: The dox-inducible A375P cells were treated with doxycycline to express MITF WT and MITF mutant proteins for 24 hours and then treated with 40 µg/ml cycloheximide (Sigma #66819), in presence or absence of 200nM TPA ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1 mL using a 50 kDa molecular weight cut-off (MWCO) centrifugal filter (Millipore, #UFC505024). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant proteins were denatured at 95℃ for 10 min and then alkylated with 40 mM IAA (final concentration, Sigma-Alrich) in dark at room temperature for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Pull down of Ago proteins (Ago 1-4) was performed by incubating 500 μg of Flag-GST-T6B peptide 111 (Sigma #M8823) with 80 μl of anti-Flag M2 Magnetic beads (Sigma #M8823 ...
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bioRxiv - Plant Biology 2023Quote: ... protein thiol groups were blocked by a final concentration of freshly balanced 20 mM N-ethylmaleimide (NEM, Sigma 128-53-0) directly added to the lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... produced in house from pAB101 hybridoma cell culture and purified on protein A Sepharose (GE)) or Ab416 (for JCPyV LT, EMD Millipore) primary antibody diluted to 0.2μg/mL in TBST for 4h at RT ...
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bioRxiv - Microbiology 2023Quote: ... from Barcelona (Spain) was filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts) to remove bacteria ...
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bioRxiv - Neuroscience 2023Quote: ... the expression of truncated tau protein was induced by cultivating cells in a medium without doxycycline (Sigma-Aldrich, St. Louis, MO) for three days before cell seeding into co-culture ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein quantification was performed using the BCA protein quantification assay (QPro BCA kit, Cyanagen) against a bovine serum albumin (BSA) calibration curve (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Expression of the different FLAG-SET8 proteins in U2OS cells were induced by the addition of 2 µg/ml of tetracyclin (TET) (Sigma-Aldrich) into cell culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques ...
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bioRxiv - Neuroscience 2023Quote: ... solubilized GluA2 or GluA2-γ2 (1 nmol) and membrane scaffold proteins (50 μL, final concentration 1 mg/mL) (MSP1E3D1, Sigma‒ Aldrich) were added ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in 4 mL of Amicon filter concentrators with a 10 kDa cutoff (Millipore Sigma; Burlington, MA). TCEP (1 mM ...
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... Equal amounts of protein were separated on a SDS-PAGE gel and transferred to an Immobilon-P polyvinylidene difluoride (PDVF) membrane (Millipore, IPVH00010). Membranes were blocked in PBS with 3% milk powder (Sigma ...
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bioRxiv - Genomics 2023Quote: ... the remaining chromatin was divided amongst separate tubes containing protein A dynabeads pre-incubated with either 2μl anti-H3K4me3 (Millipore 07-473), 10μl anti-H3K27me3 (CST 9733 ...
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bioRxiv - Molecular Biology 2023Quote: ... was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich). 50 µL of each sample was stored at 4°C while the other 50 µL was dried using a Savant SPD131DDA SpeedVac (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound samples were successively incubated with appropriate amount of primary antibodies against protein-of-interest (CTCF antibody: Millipore, 07-729) in 50µL of CUT&RUN antibody buffer for overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins eluted by 100 mM imidazole were collected and then desalted with disposable PD 10 desalting columns (Sigma, GE17-0851-01) against stocking buffer (20 mM HEPES (pH 7.3) ...
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bioRxiv - Biochemistry 2023Quote: Studies of SAMHD1-controled dynamic equilibria of dNTP concentrations (Fig. 5) were performed using a 10 mL stirred-cell pressurized protein concentrator (Amicon; EMD Millipore) assembled with a 10 kDa MWCO nitrocellulose membrane (Millipore) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was incubated for 1h at 4 °C in the presence or absence of 20 or 100 µM FTY720 (Sigma-Aldrich) or fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
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bioRxiv - Biochemistry 2023Quote: Clicked and dissolved protein samples were diluted to 4 M urea with 50 mM ammonium bicarbonate (pH 8, AmBic, Sigma-Aldrich). The proteins were reduced with 4 mM DTT (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... anti-ZEB-1 (Zinc finger E-box-binding homeobox 1, Cat. #SAB3500514, from rabbit), and anti-Snail (Drosophila embryonic protein, Cat. #SAB2108482, from rabbit) were purchased from Sigma-Aldrich. Anti-EpCAM-PE (anti-epithelial cell adhesion molecule labeled with phycoerythrin ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the phage suspensions were further filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts), diluted and plated as indicated in the previous paragraph to verify the uniformity of the plaques ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Microbiology 2023Quote: ... and the flow-through containing cleaved protein was collected and concentrated to 2 mL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Bioengineering 2023Quote: We verified the patterning and the pattern transfer to PA hydrogel using green fluorescent laminin (as described in Protein patterning glass coverslips) and a pan-cadherin primary antibody (Sigma, C3678). We diluted the pan-cadherin antibody 1:200 in PBS and incubated on the devices for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
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bioRxiv - Genomics 2024Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase conjugated secondary antibodies (table 2) were added for 1 h at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
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bioRxiv - Neuroscience 2024Quote: ... Chromatin samples were pre-cleared with 10 µl of Magna-ChIP® protein-G magnetic beads (EMD Millipore, Cat: 16-662) pre-blocked with BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies and chromatin were mixed with 20 µl of Magna-ChIP® protein-G magnetic beads (EMD Millipore, Cat: 16-662) for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Biochemistry 2024Quote: Cells were harvested by centrifugation at 2000 x g for 15 minutes at RT and resuspended in Lysis Buffer (1X BugBuster® Protein Extraction Reagent (Merck-Millipore), 20 mM Tris ...