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Citations for Millipore Sigma :
7001 - 7050 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 20 µl protein extract were separated on a 12% SDS gel and transferred to Nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). Anti-S6K1 (phospho T449 ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... ∼2×107 parasites were collected by centrifugation (1,200 x g for 10 min) and pull-downs were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Sigma-Aldrich). Unenriched ‘input’ samples were generated by reserving back 10% of the starting lysate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed twice with cold phosphate-buffer saline (PBS) and total proteins were precipitated in 5% trichloroacetic acid (TCA) with 1 mM Methionine (Met) (Sigma-Aldrich) for 15 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of each GalNAcT-FLAG enzyme was measured by performing immunoblot analysis together with standard FLAG-BAP fusion protein (Sigma-Aldrich) using the M2 antibody ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2022Quote: ... Chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated with the indicated antibodies or rabbit preimmune sera complexed with Protein A agarose beads (Sigma-Aldrich). Immunoprecipitated complexes were sequentially washed with Wash Buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Debris were later removed by centrifugation and 20 μl of the cleared protein sample were spiked-in with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl of 1.25x protein loading buffer ...
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bioRxiv - Microbiology 2022Quote: ... Bound rVSVs were detected by incubation with a streptavidin-HRP conjugate (PeirceTM, and sEC1-4 protein was detected by incubation with an anti-Flag m2 mAb-HRP conjugate (Sigma-Aldrich) (1 h at 37°C) ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cell lysates expressing FLAG-KRAS proteins were immunoprecipitated from 800 μg of cell lysate using anti-FLAG-M2 magnetic beads (Sigma, M8823) by using the KingFisher DuoPrime purification system (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reagents for protein chemistry (e.g., triethylammonium bicarbonate buffer, iodoacetamide, dithiothreitol, and formic acid) were purchased from Sigma Aldrich (St. Louis, MO). Sequencing grade trypsin was from Promega (Madison WI) ...
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged protein was precipitated with 20 μl anti-V5 beads (Anti-V5 Agarose Affinity Gel, Cat.- No. A7345, Sigma-Aldrich) rotating on a wheel for 2 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... 200μg protein was incubated with anti–c-Myc affinity gel (EZview™ Red Anti-c-Myc Affinity Gel, #E6654, Sigma-Aldrich) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... 600 μg protein equivalents of exosomes pelleted by ultracentrifugation were resuspended in 500 μl Diluent-C for membrane labeling (Sigma CGLDIL) and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET) ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples dissolved in Laemmli buffer were separated on SDS-PAGE gel and transferred to Immobilon-P PVDF membrane (Millipore, IPVH00010). Transferred protein samples were detected using following primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Soluble proteins were hatched for 10 min at 55°C with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, St. Louis, MO, USA), then alkylated for 15 min at RT in the dark with 10 mg/mL iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... the accumulated lysates were incubated with protein A/G magnetic beads (Thermo-Fisher) pre-coupled with anti-Flag antibody (M2 monoclonal antibody, Sigma-Aldrich) for 2 h at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... One mg of total protein lysate was incubated with 40ul of Ezview Red ANTI-FLAG M2 Affinity Gel (Cat # F2426-1ML, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Synthetic Biology 2022Quote: ... and both fractions were checked by SDS-PAGE followed by staining with Coomassie-based dye according to the manufacturer’s instructions (Instant Blue Protein Stain, Sigma ISB1L-1L).
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P3HT-GRAPHENE DEVICE FOR THE RESTORATION OF VISUAL PROPERTIES IN A RAT MODEL OF RETINITIS PIGMENTOSAbioRxiv - Neuroscience 2022Quote: ... ionized calcium-binding adaptor molecule 1 (Iba-1, 1:1000; Wako Pure Chemical Industries, Japan) and glial fibrillary acidic protein (GFAP, 1:200; Sigma-Aldrich) in 1% NGS and 0.3% Triton-X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Physiology 2022Quote: ... propionate and butyrate in the serum was determined by ion chromatography analysis after depletion of proteins and lipids with acetonitrile (Sigma-Aldrich). Samples were loaded on a Dionex ICS-5000 Plus system automatic device (ThermoScientific ...
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bioRxiv - Plant Biology 2024Quote: ... Active PME units per µg of protein were calculated by using a standard curve generated with known amounts of commercial PME (Sigma, P5400).
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Pathology 2024Quote: ... Normalised amounts of protein were reduced and alkylated with 10 mM TCEP (Thermo, #77720) and 40 mM chloroacetamide (Sigma, C0267-100G) with incubation at 55 °C for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... GST tags were removed by incubating 0.70 mg of protein coupled to glutathione Sepharose 4B (Cytiva) with 20 NIH units of thrombin (Sigma-Aldrich; 10602400001) overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked and permeabilized in protein-blocking buffer (DPBS with 2% BSA, 10% fish gelatin (cat#: G7041, Sigma-Aldrich), 0.2% Triton-X (cat# T9284 ...
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bioRxiv - Microbiology 2024Quote: ... The protein-containing fractions were pooled and concentrated in an Amicon spin concentrator of 10-30kDa cutoff (Millipore, UCF901024 and UCF903024) and buffer exchanged against Buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... correct production of the protein of interest was checked by Western Blot of HEK293T-transfected cells using an anti-Flag antibody (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... For LC-MS/MS analysis protein gels were stained over night at room temperature with Coomassie solution (B8522-1EA; Sigma-Aldrich) and washed with 2.5% acetic acid and 20% methanol and subsequent washes of 20% methanol ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... isolated mitochondria equivalent to 1 mg protein were mixed with 250 μl methanol before adding 5 mL 0.1M Hydrochloric acid (Sigma, Cat# 1090601000) and 100 µL SPLASH (Avanti Polar Lipids ...
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bioRxiv - Cell Biology 2024Quote: ... Equal concentration of total protein extracts were incubated with Myc (#1-9E10.2 hybridoma supernatant, ATCC, USA) or HA (#11583816001, 12CA5, Sigma-Aldrich, USA) antibodies for 2h at 4°C on a rotator ...
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bioRxiv - Cell Biology 2024Quote: ... Equal quantities of proteins (20 μg/lane) were loaded onto 8% or 10% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore, USA). The membranes were then incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
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bioRxiv - Immunology 2024Quote: ... 1 μl of serum sample (∼70-80 μg protein) was lysed with 0.1% Rapigest (Waters, MA, USA) in 100 mM ammonium bicarbonate (Sigma, MO, USA) and denatured at 95°C for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 nL of 12.5 ng/µL of sgRNA and 1 nL of Cas9 protein (300 ng/µL)64 along with phenol red (Sigma Aldrich) were co-injected in one-cell stage embryos ...
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bioRxiv - Microbiology 2024Quote: ... The eluted fractions from solution C (containing target proteins) were applied to an Amicon Ultra 15 Centrifugal Filter (Millipore Billerica MA) for desalting and imidazole removal by using a buffer containing 100 mM Tris–HCl (pH 7.9) ...
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bioRxiv - Biochemistry 2023Quote: For cMTHFR crystallization in the R-state, purified protein (cMTHFRE21Q, L393M, V516F) underwent treatment with formaldehyde and dimethylamine-borane complex (Sigma-Aldrich) for surface lysine methylation31,32 ...
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bioRxiv - Cell Biology 2024Quote: ... A pair of gRNAs with minimum off-target and maximum on-target activity were designed and selected to specifically target sequences that encode essential protein domains of the Bend2 gene using CRISPR DESIGN TOOLS (Millipore Sigma) (gRNA1-antisense ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates from cell lines were prepared using the CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, St. Louis, MO, USA), while CelLytic™ MT Mammalian Tissue Lysis Reagent (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Plant Biology 2023Quote: The sequences encoding mature proteins were cloned into vector pET-15b with an N-terminal His6 tag sequence (Novagen, Darmstadt, Germany) (primer sequences ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µg of protein per sample were digested in a solution containing 100mM triethylammonium bicarbonate at pH 8.5 (TEAB) (Sigma Aldrich, T7408), benzonase nuclease (Millipore Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich, I1284). Bacteria pellets were resuspended in Bugbuster (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Isolated adipocytes obtained as described above were washed in PBS three times and proteins were extracted from 500 μl of adipocyte with 500 μl 1X RIPA buffer (Sigma-Aldrich) supplemented with complete antiprotease tablet (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... briggsae ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 400 μl with (10 mM Tris-HCl ...