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Citations for Millipore Sigma :
6951 - 7000 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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bioRxiv - Physiology 2021Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP and Iba-1 protein analyses were generated from previously collected data and normalized to GAPDH (1:5000; cat# G9545, Sigma-Aldrich) [13] ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 8 - 20 mg/mL (Amicon Ultra 10 kDa cut off, Millipore Sigma). Protein samples were stored at 4°C until use.
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
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bioRxiv - Microbiology 2021Quote: The heat extracted proteins from OD600=2 culture was suspended in 1X Laemmli buffer with 250 U of benzonase nuclease (Sigma E0114) and incubated for 5 minutes at 95°C ...
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bioRxiv - Cell Biology 2019Quote: ... Dehydration of gel pieces was done by addition of 100% ethanol followed by protein in-gel digestion with trypsin (Sigma-Aldrich) at 37 °C for 16 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... RIPK1 kinase was performed on KISSf screening facility (IBISA, Biogenouest, Station Biologique, Roscoff, France) with myelin basic protein (MBP, Sigma, #M1891) as substrate and in the presence of 15µM ATP.
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bioRxiv - Plant Biology 2020Quote: ... MPK purification or modification (e.g., dephosphorylation or protease digestion) was followed by protein desalting using Amicon Ultra filters (Millipore, Billerica, MA) to remove HCO3- and other salts and buffers.
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... following the SDS-PAGE and washing of the excised gel slices proteins were reduced by adding 10 mM Dithiothreitol (Sigma Aldrich) prior to alkylation with 55 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... precleared cell extracts were incubated with the indicated antibody for 4 hours at 4°C with rotation followed by 1 hour of pull-down by 1:1 protein A/G agarose beads (GE, 17061801, 17127901) or FLAG/Myc beads (FLAG resin: Sigma, A2220; Myc resin: Sigma, E6654). Immunoprecipitates were washed with lysis buffer three times before electrophoresis.
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bioRxiv - Neuroscience 2021Quote: ... Proteins extracted from the brain tissues were separated by SDS-PAGE and electro-transferred to a PVDF membrane (Millipore, Bedford, MA). The membrane was blocked with 2% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of protein from each sample was incubated for two hours with purified Multidsk2 recombinant protein bound to glutathione agarose magnetic beads (Sigma G0924). After incubation beads were washed four times with D-buffer and resuspended in NuPage LDS sample buffer (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of the NK1R-miniGs399fusion protein was induced and enhanced 18 hours after transfection with addition of 1 μg/mL doxycycline hyclate (Sigma Aldrich), 10 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... to preclear samples for 2 hours and subsequently incubated with Dynabeads protein G coated with an anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Fifty micrograms of protein was fractionated via 10% SDS-PAGE and transferred to Immobilon Transfer Membranes (Millipore Corporation, Billerica, MA, USA). Primary antibodies used in this study included those against β-actin (GTX109639 ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... the TEV cleavage step was done overnight at 4°C and the cleaved proteins were concentrated using 100K MWCO concentrator (EMD Millipore). Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore ...
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from cells using Trizol® and protein was isolated from cell lysates with RIPA buffer (Sigma-Aldrich®) containing Triton X100 1% and SDS 0.1% ...
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bioRxiv - Molecular Biology 2021Quote: ... The purity of the protein after each step was assessed by 12% SDS-PAGE stained with Coomassie Brilliant Blue R 250 (Sigma-Aldrich). First ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of protein was reduced with 10 mM dithiothreitol (DTT; FUJIFILM Wako) and alkylated with 40 mM 2-chloroacetamide (CAA; Sigma-Aldrich). Digestion was performed according to the PTS protocol (Chang et al. ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Microbiology 2021Quote: ... The FLAG-tagged protein-DNA complex was purified via affinity chromatography using anti-FLAG M2 affinity gel (Sigma-Aldrich, Taufkirchen, Germany). The purified protein-DNA complexes and the control were incubated at 95 °C for 40 min for reversal of the crosslink ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The covalently fluorescently labelled nucleosome arrays used for microinjection were labeled at an 8% fluorophore density (8 in 100 histone2B proteins labeled with a fluorophore) and dialyzed against TE (10 mM Tris-HCl (Sigma-Aldrich), pH 7.4 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Genetics 2020Quote: ... proteins were eluted by boiling in 1XSDS-PAGE loading buffer followed by immunoblotting analysis using anti-FLAG antiboy (Sigma; Cat# A8592).
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bioRxiv - Microbiology 2020Quote: ... The temperature was subsequently reduced to 18°C and protein production was induced with 0.5 mM Isopropyl-β-D-thiogalactoside (IPTG, Sigma Aldrich) overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged and protein concentration measured using a bovine serum albumin standard curve and a bicinchoninic acid (BCA) assay (Sigma-Aldrich). The WES capillary electrophoresis system (ProteinSimple ...
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bioRxiv - Biochemistry 2020Quote: The effect of the mutations on translational control in response to stress was assessed by measuring the incorporation of puromycin into newly synthesized proteins by immunoblotting lysates of untreated and thapsigargin (Sigma, T9033) (200 nM ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Conditional degradation of mAID tagged EWSR1 protein in the AUX treated OsTIR7-19 cells was confirmed by Immunocytochemistry and Western blot analysis using anti-FLAG (Sigma, #F7425) and anti-EWSR1 (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... purified the protein using Ni- resin and exchanged the bound nucleotide for non-hydrolysable GTP analogue GMPPCP using alkaline phosphatase beads (Sigma-Aldrich) and following the protocol of John et.al ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were fractionated by 10-15% SDS gels and electro-transferred to polyvinylidene difluoride transfer membranes (#IPVH00010, Millipore, Billerica, MA, USA). The membrane was incubated overnight with primary antibodies and then with secondary antibodies conjugated with horseradish peroxidase (#7074S and #7076S ...
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bioRxiv - Biochemistry 2022Quote: ... and the major fractions containing the target protein were pooled and then concentrated with an Amicon® Ultra-15 Centrifugal Filter Unit (10 kDa cutoff, Millipore) to ~500 mL ...
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bioRxiv - Bioengineering 2022Quote: ... hexane-defatting was performed before extraction by adding 10 g of protein meal to 100 mL of hexane (Sigma #270504-1L) and incubating the mixtures at room temperature on a stir-plate for one hour ...
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bioRxiv - Cancer Biology 2022Quote: Thrombin (human plasma, high activity) and trypsin (porcine pancreas, Type IX-S, 13000-20000 BAEE units/mg protein) was purchased from Millipore Sigma. PARs activating peptides TFLLR-NH2 (PAR1) ...
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bioRxiv - Cell Biology 2022Quote: ... and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8, 10 mM glutathione (Sigma-Aldrich; G4251). The supernatant was separated from the resin to a fresh tube ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (# C10428, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the beads were removed and protein of interest was concentrated using a 100 kDa molecular weight cut-off (MWCO) concentrator (Millipore, #Z648043) to 1 mL and flash-frozen in liquid nitrogen.
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bioRxiv - Developmental Biology 2022Quote: ... Activity of the TCF3-VP16GR chimeric protein was induced by incubating the embryos for 24 hours in 4 μg/ml dexamethasone (DEX, Sigma-Aldrich) from stage 37/38 to stage 41.
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).