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Citations for Millipore Sigma :
7201 - 7250 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Approximately 750 μg of total protein lysate per pull-down was bound to 50 μl of Flag-M2 magnetic beads (Sigma-Aldrich, USA) in binding buffer (lysis buffer but with 1%-NP-40 ...
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bioRxiv - Synthetic Biology 2019Quote: ... loaded sensors were equilibrated in kinetics buffer and incubated with either 5 nM or 25 nM HER2 protein (Sigma-aldrich, SRP6405-50UG). Lastly ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then process for mass spectrometry or the proteins were transferred to the polyvinylidene difluoride membrane (Merck Millipore, Cat. No. IPVH00010) by Western Blotting (WB) ...
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bioRxiv - Systems Biology 2020Quote: ... coli K12 whole cell lysates were analysed using 10% Mini-PROTEAN Protein Gels (4568034, Bio-Rad, Laemmli buffer S3401-VL, Sigma Aldrich). One part was stained using Coomassie Blue for total protein content (Brilliant Blue G solution ...
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bioRxiv - Synthetic Biology 2021Quote: To induce the degradation of mAID-fused proteins, cells were treated with 500 µM indole-3-acetic acid (auxin, IAA, dissolved 500 mM in water) (Millipore Sigma, I5148) for 1 hour ...
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bioRxiv - Biophysics 2021Quote: Wild type and p75NTR KO CGNs were trypsinized and plated at a density of 40 000 (for cell death assay) or 200 000 (for protein collection) cells per coverslip coated with poly-L-lysine (Sigma, Cat: P7280) in a 24-well (Starlab ...
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bioRxiv - Bioengineering 2021Quote: ... Western blot analysis was performed using a primary antibody of rabbit anti-protein A (1:25,000 dilution) (Sigma-Aldrich, St. Louis, MO) and a secondary antibody of goat anti-rabbit IgG-HRP (1:3,000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS at 37°C for 30 minutes and subsequently incubated for two hours with a 1:200 concentration anti-glial fibrillary acidic protein (GFAP) antibody produced in mouse (Sigma, 1:200) and 1:500 concentration anti-β-Tubulin III antibody produced in rabbit (Sigma ...
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bioRxiv - Pathology 2021Quote: HIF-1α protein expression was depleted in human U937 cell line (ATCC, USA) using shRNA targeting HIF-1α (TCRN0000010819, Sigma Aldrich, USA) using a liposome mediated delivery (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The GST-Cdc14 constructs were expressed at 16°C overnight and the protein purification followed the same procedure as for 6His-3Flag-Cdc14 using glutathione-Sepharose beads (Millipore-Sigma, 17075601) and the following buffers ...
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bioRxiv - Plant Biology 2021Quote: Purified filaments were resuspended in SDS-PAGE sample buffer and filament proteins were subjected to SDS-PAGE on 4-20% TruPAGE Precast Gels (Sigma-Aldrich, USA). Gels were stained with Coomassie brilliant blue G-250 stain (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... An efficient transfer of proteins was determined by staining a membrane with Ponceau S solution (Sigma - Aldrich, Cat No 6226-79-5). Afterwards a membrane was incubated for 1 h in a blocking buffer shaking at room temperature followed by binding with primary antibody in 5 % BSA/TBS-T buffer overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used to detect the following proteins: GFP (1:200, CST, cat. # 2956,), peripherin (1:1000; Novus, cat. # NBP1-05423, NF200 (1:500, Millipore, cat # MAB5266). Primary antibodies were applied and incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and Zbtb16 (1:250, Santa-Cruz, Mississauga, ON) Beta-actin was used as a reference protein (1:5000, Sigma-Aldrich, Oakville, ON). Blots were scanned by Amersham Imager 600 imaging system (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: Transiently expressed antibodies were purified from 30 ml of HEK 293 Expi cultures using Protein G GraviTrap columns (Sigma, GE28-9852-55) and the Ab Buffer Kit (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated in 4-15% polyacrylamide gels and transferred to 0.2 µm Immobilion-PSQ transfer membrane (Millipore, Cat. No ISEQ00010) using a Bio-Rad transfer system ...
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bioRxiv - Cancer Biology 2020Quote: Detection of an interaction between BRCA1 and the cavin or CAV1 proteins was assessed using the Duolink™ II Detection Kit (Sigma Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2020Quote: ... Uncojugated FITC was separated from the labelled proteins using PD-10 spin columns (Sigma Aldrich, PD Spintrap™ G25, #GE28-9180-04) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... About 20 ug total proteins in each sample were fractionated by 10% SDS PAGE gel and transferred to PVDF membranes (Millipore Corporation, USA) and immunoblotted with primary antibody anti-GFP (Invitrogen-Molecular Probes ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were separated using a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred onto Immobilon-FL PVDF membrane (Millipore). The membranes were blocked in 5% BSA for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation and 400 μg of protein lysate were incubated with 40 μL of Anti-FLAG® M2 affinity gel (Sigma, A2220) for 2 h with end-over-end rotation at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells used for total protein purification were lysed with 2 ml of a buffer containing 1% Sodium dodecyl sulfate (SDS; Sigma-Aldrich #436143), 50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... 15 μg of tumor protein was resolved on 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membrane (Millipore, catalog# IPFL00010). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Cell Biology 2022Quote: ... the proteins were wet transferred onto ‘Amersham™ Protran™0.2 µm NC’ nitrocellulose membranes (GE10600002 Sigma-Aldrich Handels Gmbh, Vienna, Austria) at constant 80 V for 1,5 h ...
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bioRxiv - Developmental Biology 2022Quote: ... SDS-PAGE and western blot analyses were used to detect proteins and modifications using the following antibodies: anti-phospho SMAD2 (Ser465/467, Sigma, 1:500), Smad2 Polyclonal Antibody (Life Technologies 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Neuroscience 2022Quote: ... The reporter proteins were released from the ER-localized streptavidin ‘hook’ with the addition of 40 μM biotin (Sigma-Aldrich, B4639-100MG) to the coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein from each sample were loaded into 10-20% Tricine sodium dodecyl sulphate– polyacrylamide gel electrophoresis (SDS-PAGE; Sigma-Aldrich, Sweden), followed by immunoblotting on polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.1 (final urea concentration < 2 M) before addition of trypsin (1 µg per 50 µg of protein; Sigma-Aldrich Cat# T6567) for a 3 h incubation ...
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bioRxiv - Biophysics 2022Quote: ... about 100 μL supernatant was left and the bound protein was eluted by incubation with 10 μL FLAG peptide (Sigma-Aldrich F3290) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Refolded CTSL-YLAL-C25S and CTSL-C4S-C25S proteins were concentrated and purified further by gel-filtration chromatography on a Sephacryl S-100 (Sigma-Aldrich, USA) column that had been pre-equilibrated with 20 mM Tris–HCl pH 8.0 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Molecular Biology 2021Quote: ... each sample with equal amount of protein was subjected to SDS-PAGE gel (10% or 15%) and transferred to a PVDF membrane (Millipore, Massachusetts, USA). The membrane was incubated with the specific primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: For phosphorylated proteins RIPA lysis buffer was further supplemented with PIC and 1X phosphatase inhibitor cocktail (Merck Millipore, cat no. 524629-1ML). A cocktail of phosphatase inhibitors (B-Glycerolphosphat (25mM) ...
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bioRxiv - Neuroscience 2021Quote: ... standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or μ rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 900 μl of 25% trichloroacetic acid (TCA) before harvesting precipitates on Whatman filter strips (Sigma-Aldrich, Cat: WHA1823035). Filter paper disks were placed in Biofluor scintillation fluid (Perkin Elmer ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal loading was verified in a twin run of electrophoresed individual strips of protein stained with Ponceau S (Sigma, St-Louis, MO) and extracted with PBS supplemented with 1.125 M NaCl and 0.6% Tween-20 (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Microbiology 2022Quote: ... Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore, cat#UFC805024). The concentrated sample was stored in 50% glycerol at −20°C.
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at >10,000 rpm to remove cellular debris and the supernatant containing extracted protein was transferred to a black flat-bottom microwell plate (Millipore Sigma, M9685). Relative fluorescence intensity of each sample was measured on a SpectraMax iD3 microplate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Systems Biology 2019Quote: ... previously adapted to serum free suspension culture were propagated in a DMEM/F12-based protein free chemically defined medium (PFCDM) supplemented with Soybean peptone (Catalog number P0521, Sigma-Aldrich, USA) and HT supplement (Gibco™ catalog number 11067-030 ...
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bioRxiv - Molecular Biology 2019Quote: ... The Est1:Ku80 interaction was analyzed as above except 4 mg of protein was incubated with 30 μL α-FLAG M2 magnetic beads (Sigma M8823) and samples were analyzed by SDS-PAGE on a 10% gel.
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bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...