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Citations for Millipore Sigma :
7351 - 7400 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were concentrated and exchanged with phosphate-buffered saline (PBS) pH7.4 using an Amicon YM-10 centrifuge concentrator (Millipore, Bed-ford, MA).
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bioRxiv - Plant Biology 2023Quote: ... Supernatants containing soluble proteins fraction were transferred in a new Eppendorf tube of 1.5mL and the total protein concentration was determined by BRADFORD assay (Sigma-Aldrich, ref 23238).
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Immunology 2023Quote: ... To ensure efficient and reproducible protein immobilization a preliminary array validation was carried out with anti-FLAG antibodies (Sigma-Aldrich, cat# F7425) 1:5000 and mouse anti-His6 tag polyclonal antibodies (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The prepared proteins were separated by SDS-PAGE gel electrophoresis (Yazyme, Shanghai, China) and transferred to activated PVDF membranes (Millipore, MA, USA). The PVDF membranes were then gently rinsed with TBST and placed in 7% skim milk powder solution and closed on a shaker at room temperature for 3 h ...
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bioRxiv - Pathology 2023Quote: ... An equal amount of protein (10 µg) was subsequently separated by 12% SDS-PAGE and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Bedford, MA). The membrane was blocked with a 5% skimmed milk solution prepared in Tris-buffered saline containing 0.1% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The viral pellet was then lysed in Western blot lysis buffer with 4x Protein Sample Loading Buffer (LICOR) supplemented with 10% β-mercaptoethanol (Sigma Aldrich) and heated at 95°C for 5 min.
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 40 μl of Protein A/G magnetic beads was incubated with 5 μg anti-pan-ADP-ribose binding reagent (Millipore Sigma, MABE1016) per IP sample in 100 μl PBS for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2023Quote: ... For each lane we treated 15-20 μg protein samples with 4X NuPAGE LDS Sample Loading buffer (Thermo #NP0007) and 5% 2-mercaptoethanol (Sigma #M3148-25ml), heated them at 70°C for 6 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 hours at RT and then immunoblotted overnight at 4°C with the primary mouse anti-amyloid precursor protein A4 antibody (clone 22C11) (Sigma MAB348-AF647) (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... active and inactive NS2B(H)-NS3 pro proteases were generated as described previously and cloned into the MCS1 (multiple cloning site 1) of dual protein expression plasmid pETDuet-1 (Novagen, Beijing, China) through restriction sites EcoRI and NotI ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were visualized by Coomassie-stained SDS-PAGE and concentrated using a 3 kDa MWCO Amicon Ultra centrifugal filter (Millipore Sigma, #UFC9003,).
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bioRxiv - Immunology 2024Quote: ... Proteins from total cell extract were quantified using BCA reagent according to manufacturer instructions (Sigma-Aldrich, Humeau, La Chapelle-Sur-Erdre, France). A standardized amount of protein extracts was treated with Laemmli buffer containing 10 mM DTT and heated at 95 ° C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 15µg of each protein sample was prepared with Laemmli Sample Buffer (Bio- Rad cat. 1610737) and 50mM DTT (Millipore Sigma cat. 43816). Samples were loaded on a 4-15% Criterion TGX Protein Gel (Bio-Rad cat ...
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bioRxiv - Biophysics 2024Quote: ... γ-cyclodextrin (γ-CD, CAS#7585-39-9), C-reactive protein (CRP, AG723) and human serum (H4522-100ML) were ordered from Sigma-Aldrich. 1 µM γ-CD stock suspension was prepared with MilliQ ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... 450 µg of total protein were incubated for 20 min (rotating wheel, RT) with 50 µL of streptavidin-agarose beads (Sigma-Aldrich, #S1638). After incubation ...
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bioRxiv - Neuroscience 2024Quote: ... An antibody reactive for the N-terminal amino acids 66-81 of the amyloid precursor protein (APP) (Clone 22C11: Millipore, Billerica, MA) was applied at 1:40K and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML) beads at 4°C for 1.5 hours while inverting ...
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bioRxiv - Molecular Biology 2024Quote: ... to alkylation with IAM 200mM and to complete protein digestion with 1 μg of Trypsin (Sigma-Aldrich Inc., St. Louis, MO, USA). The peptide digests were desalted on the Discovery® DSC-18 solid phase extraction (SPE ...
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bioRxiv - Immunology 2024Quote: ... These diluted test peptides were co-incubated with 0.1 μM biotin-labeled peptide and 50 nM purified MHC-II proteins (ProImmune, Oxford, United Kingdom) at 37°C for 24h in the presence of Octyl-β-D-glucopyranaside (Sigma-Aldrich). Then ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was cut across the molecular mass region of the corresponding proteins and separately probed with the following corresponding antibodies: an anti-β-glucuronidase (N-terminal) antibody (G5420, Sigma‒Aldrich) and an anti-tubulin antibody (T5168 ...
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bioRxiv - Molecular Biology 2024Quote: ... Pooled lysate was subsequently split into 2×200 μL aliquots and protein was enriched by adding 20 μL anti-flag (Sigma cat#M8823) or anti-myc (Fisher cat#PI88842 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled lysate was divided into two 350 μL aliquots and protein was enriched by adding 35 μL anti-flag (Sigma cat#M8823) or anti-pErk beads (see ‘Basal Activity Scan’ for instructions on generating pErk beads) ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
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bioRxiv - Genomics 2024Quote: ... Protein was purified by Nickel-NTA resin and treated with TEV protease (1mg lab made TEV per 40mg of protein) and benzonase-100units/ml (Novagen, 70664-3) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluates were resolved on a 10% SDS-PAGE gel and the proteins were electroblotted onto PVDF membranes (Millipore, Immobilon-P, Cat # IPVH20200) for probing with following primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty micrograms of total protein were separated on 10% SDS- PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore Co., Bedford, MA). Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... kinase assays were performed by incubating the immunoprecipitated proteins and beads in 30 μl kinase buffer supplemented with 50 μM ATP and 3 μg histone H1 (EMD Millipore, 14-155) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... An even protein transfer was verified by reversibly staining membranes with Poncaeu S solution (Sigma-Aldrich, 0.1% w/v in 1% acetic acid). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein was separated by 12% sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) electrophoresis and then was transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA), which was followed by incubation with antibodies against STAP1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-10 μg protein lysates were cultured and proteasome activity measured using the 20S Proteasome Activity Assay kit (APT280, Millipore, Billerica, MA, USA) per manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2019Quote: ... cells were harvested between 24 and 48 h post-transfection and lysed with M-PER Mammalian Protein Extraction Reagent (Pierce Biotechnology) containing a mammalian protease inhibitor cocktail (SIGMA P8340, Sigma-Aldrich) according to the protocol of the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... A total of 70 μg of protein was subjected to electrophoresis and then transferred to nitrocellulose membranes (Sigma Chemical Co., St. Louis, MO). The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Physiology 2019Quote: ... equal amounts of protein (in 1X Laemmli loading buffer) were resolved using SDS-PAGE gels and transferred to PVDF membranes (Millipore Cat. No# IPVH00010). After blocking in 5% BSA or 5% fat-free milk in TBST (TBS with 0.1% Tween-20 ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were induced to express the indicated BioID2-tagged proteins by the addition of 0.2 μg/ml doxycycline (Sigma-Aldrich, St. Louis, MO) for 16 hours ...
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bioRxiv - Bioengineering 2020Quote: ... cells were pelleted by 10 minutes of centrifugation (4122 RCF, 4 degrees C) and lysed with BugBuster protein extraction reagent (Millipore Sigma, Catalog #70584) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on whole-mounted retinal sections were brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID:AB_94166) at 1:1000 and GFAP (1:1000) ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: Cell culture supernatant was diluted 1:1 in binding buffer (0.16 M sodium phosphate, 500 mM NaCl, pH 9) and loaded on a Protein A Sepharose (Sigma Aldrich, 17-1279-03) column at a flow rate of 2 ml/min ...
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bioRxiv - Microbiology 2021Quote: ... Buffers were then exchanged to PBS and proteins were concentrated by 30 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore, Cat. No. UFC903096).
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...