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Citations for Millipore Sigma :
7301 - 7350 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: The nucleic acid binding property of protein was further validated using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 μgm recombinant protein was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were then incubated overnight at 4ºC with primary antibodies for myelin basic protein (1:250 Millipore Cat# MAB384-1ML, RRID:AB_240837), neurofilament 160 kD medium (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... vesicular fraction from brains obtained as described earlier was first pre-cleared for an hour at 4°C with protein A Sepharose beads (Sigma Aldrich-P9424) and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant that contained the soluble fusion protein was filtered over Millex-GP syringe-driven 0.22-µm PES membranes (Millipore Sigma, Burlington, MD). The fusion protein was purified by fast protein liquid chromatography (FPLC ...
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bioRxiv - Plant Biology 2024Quote: ... the total proteins in the samples were measured through the BiCinchoninic acid Assay using a kit (Sigma-Aldrich, Saint Louis, MO, USA). The assay was conducted according to the method provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in samples was determined using Bradford assay (Bradford, 1976) against standards of known protein concentration prepared using bovine serum albumin (BSA; Sigma-Aldrich, UK).
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bioRxiv - Microbiology 2024Quote: ... the dried protein pellet was dissolved in 50 µL of 8 M urea (Cytiva, 17131901) in 100 mM NH4HCO3 (ABC; Sigma-Aldrich, 11213) and 5 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then run on NuPAGE 4-12% Bis-Tris Protein Gels (Thermo #NP0335BOX) and transferred to a PVDF membrane (EMD Millipore #IPVH00005). Blots were probed with primary antibodies overnight at 4°C in 5% blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples were separated on ammediol SDS-PAGE [30] and transferred to a PVDF membrane (polyvinylidene difluoride, EMD Millipore, Burlington, MA, USA) in transfer buffer (10 mM N-cyclohexyl-3-aminopropanesulfonic acid ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates with anti-FLAG antibody were then incubated for additional 30 min (4°C, rotating) with 75 µl/sample pre-washed (3 times in buffer A) Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µg proteins were separated by SDS-PAGE (10– 12% gels) and were blotted onto a nitrocellulose membrane (Millipore, Billerica, MA, USA). The membrane was blocked with 5% BSA in TBST at room temperature for 1h ...
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bioRxiv - Biophysics 2024Quote: ... 20 µL volumes of protein were plated on 35-mm dishes precoated with 1% Pluronic F-127 (Sigma Aldrich, St. Louis, Missouri). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: The concentration of both activated proteins—PA-BFP and SumireF—was determined by the bicinchoninic acid assay using the QuantiPro™ Kit (Sigma–Aldrich), following the supplier’s instructions ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were harvested and 0.45 µm filtered before loading on to a 1 ml HiTrap™ Protein G HP column (Cytiva) pre-equilibrated in PBS (79382, Sigma-Aldrich). The column was washed with PBS and bound proteins were eluted using 5 ml of 0.1 M glycine pH 2.5 into 1 ml of 1 M Tris pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The HOS-TMPRSS2 cells that stably express Rhinolophus bat ACE2 were generated as described below (see “Generation of HOS-TMPRSS2 cells stably expressing a variety of ACE2 proteins” section) and maintained in Dulbecco’s modified Eagle’s medium (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... SG13009 colonies containing the appropriate species D pQE fiber knob protein expression vectors were cultured to an optical density of 0.6 in Terrific Broth (TB, Sigma-Aldrich, Gillingham, UK), harvested by centrifugation and purified by nickel immobilised metal affinity chromatography (IMAC ...
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bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Neuroscience 2023Quote: ... 40 μg of each protein sample was reduced with 5 mM tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich, Cat No: C4706) and then diluted in 50 mM Tris HCl ...
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bioRxiv - Microbiology 2023Quote: ... the protein was diluted tenfold into temperature-equilibrated deuterated buffer made by lyophilizing 1x PBS and resuspending in D2O (Sigma-Aldrich 151882). Samples were quenched at each time point (15s ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Physiology 2023Quote: ... The precipitated protein samples were resuspended in 100 µl of 100mM triethylammonium bicarbonate (TEAB) buffer pH 8.5 (Sigma-Aldrich cat no - T7408).
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 10 min (relative centrifugal force 12 879 g) and protein concentration was measured using the Bradford reagent (Sigma-Aldrich, USA). A protein calibration curve was determined with bovine serum albumin dissolved in a lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... 100 ng purified recombinant CTR1-KD or 1 µg MPK4 was mixed with 2 µg Myelin Basic Protein (MBP, a common kinase substrate, Sigma Cat. M1891) and 1 mM ATPγS in kinase reaction buffer (10 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... ProteoExtract® native membrane protein extraction kit and 10 kDa Amicon® Ultra-15 centrifugal filters were purchased from Millipore Sigma (USA). Assays for measuring cell toxicity were purchased from Promega (Madison ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were separated by SDS-PAGE using 10% agarose gel and transferred to transfer membranes (Millipore-Sigma, St. Louis, MO, USA), which were incubated in 5% nonfat milk (A600669 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were resuspended in cold Ripa buffer supplemented with 1% n-Dodecyl-ß-D-maltoside and complete ethylenediaminetetraacetic acid (EDTA)-free protein inhibitors (Sigma-Aldrich), and incubated at 4°C for 1 hour ...
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bioRxiv - Genomics 2023Quote: ... the anti-TOMM20 antibody and the ConA protein were functionalized for click chemistry with the addition of a NHS-SS-DBCO (DBCO) (Sigma Aldrich #761532) molecule that subsequently reacted with the azide groups linked to their respective fluorophores ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Biochemistry 2022Quote: ... for knockouts)37 containing the Che9c phage RecT annealase and Bxb1 integrase was electroporated into electrocompetent Msmeg cells (mc2155 strain73) and protein expression was induced with 500 ng/mL anhydrotetracycline (ATc, Sigma, cat. #31741). For chromosomal tagging ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted protein was concentrated in an Amicon Ultra-15 50 KDa molecular mass cut-off centrifugal filter unit (Millipore, Burlington, MA, USA) and further purified by SEC on a Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Disulfide formation was determined by injecting protein at 1.5 mg/mL in the presence and absence of 50 mM TCEP-HCl (Millipore Sigma 646547-10X1ML) and detecting the mass shift.
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Immunology 2023Quote: ... The eluted proteins were buffer exchanged into PBS and then concentrated using Amicon® 30 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9030).
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bioRxiv - Biochemistry 2023Quote: Tardigrade proteins were extracted using a modified acid extraction.35 Tardigrades were incubated in concentrated trifluoroacetic acid (Sigma Aldrich, St. Louis, MO) for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Glycine and Tris-HCl in the protein solution were replaced with PBS three times using centrifugation with Amicon Ultra-15 Centrifugal Filter Unit (50K) (Millipore, Cat# UFC905024). Protein concentrations were determined using a NanoDrop spectrophotometer (Thermo Scientific).