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Citations for Millipore Sigma :
6901 - 6950 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... Radical formation in HepG2 cells was measured by ESR spectroscopy in combination with the spin trapping technique using 50 mM 5,5-dimethyl-1-pyrolline N-oxide (DMPO, Sigma-Aldrich). Protein oxidation was confirmed via a protein carbonyl assay and oxidative DNA damage via detection of this using the Fpg comet assay (Deferme et al ...
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bioRxiv - Systems Biology 2019Quote: ... 0.01 g/l CaCl2 ×2H2O) were supplemented with 5 g/l Peptone N-Z-Soy BL 7 (Sigma-Aldrich S.r.l) (complex medium ...
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bioRxiv - Physiology 2019Quote: ... IHC was performed by incubating sections (n=5 per each group) with the primary antibody (anti-mUCP-1, U6382, Sigma), followed by a secondary antibody conjugated with HRP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Six μm sections of formalin-fixed paraffin-embedded tissue from the cortex frontal (n=3) were used to stain NeuN (Millipore), IBA1 (Wako) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: ... Next day strains were diluted to an OD600 of 0.05 in fresh glucose M9 medium containing N-acyl homoserine lactone (AHL, Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 12.5 μg/ml Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophos-phate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and lysed in 300 μl of lysis buffer (20mM Tris pH=8.0, 150mM NaCl, 1% Triton) containing 100 μM of N-Ethylmaleimide (Millipore-Sigma) and 1Xprotease inhibitor cocktail (Biotool ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Pathology 2021Quote: ... rats in the high-dose 7-d L-DOPA priming group (n=6) were treated daily with L-DOPA (12 mg/kg) + benserazide (15 mg/kg, s.c., Sigma-Aldrich) for 7 d (i.e. ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Cell Biology 2019Quote: ... and plasma (n=6) of the experimental groups were filtered with a 30 kDa cut-off Microcon filter (Merck Millipore, Tullagreen Carrigtwohill Co.Cork ...
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Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... Griess reagent was prepared in-house by mixing equal volumes of 0.1% N-(naphtyl)ethelenediamine (NED) (catalog no. 222488; Sigma-Aldrich) in distilled water with 1% Sulfanilamide (catalog no ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Developmental Biology 2019Quote: 6 dpf Cntnap2a-positive fish were treated for 1 hour with 0.05% DASPEI (2-4-dimethylamino-N-ethylpyridinium iodide; Sigma Aldrich), which labels both superficial and canal neuromasts [46] ...
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bioRxiv - Cancer Biology 2019Quote: ... RIPK3 inhibitor GSK 872 (N-(6-(Isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine) was purchased from Millipore Sigma and frozen stocks were maintained in DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM NaF (Nacalai tesque), 20 mM N-ethylmaleimide (NEM, Nacalai tesque) and 0.25 mM phenylmethylsulfonyl fluoride (PMSF, Sigma-Aldrich). The cells were washed twice with ice-cold PBS and incubated with an appropriate volume of CSK buffer for 1 hour on ice with occasional mixing ...
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bioRxiv - Bioengineering 2021Quote: ... the BMOs were placed in an increasing sucrose solution (7.5 % for 1 h, 15 % for 1 h and 30 % O/N; Sigma, S1888) for improved cryoprotection ...
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bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
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bioRxiv - Biochemistry 2021Quote: ... Proximity ligation reaction was followed by O/N decrosslinking at 65°C in the presence of 250 μg/ml proteinase K (Eurobio) and 5 mM EDTA (Sigma).
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bioRxiv - Immunology 2020Quote: ... M and N proteins were concentrated and buffer changed to phosphate buffered saline (PBS) using Microcon-10 KDa centrifugal filter units (Millipore). For N-terminal (residues from 43 to 180 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of each culture supernatant were mixed with 50 μL of Griess reagent [1:1 (v/v) 0,1% N-(1-naphthyl) ethylenediamine dihydrochloride (NEED, Sigma-Aldrich) in water ...
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bioRxiv - Immunology 2021Quote: Elastase activity was determined by the chromogenic substrate N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide (M4765, Sigma-Aldrich, USA). Supernatants were used for measuring elastase activity ...
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... fresh-frozen tissue sections were fixed in 4% paraformaldehyde in HBSS overnight at 4°C and treated with 0.2 N HCl for 20 minutes and subsequently with 3 μg/ml Proteinase K (#3115887001, Sigma-Aldrich) for 7 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
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bioRxiv - Immunology 2021Quote: Transformed bacteria were grown overnight (o/n) in 5 mL of 2x YT (tryptone + yeast extract + NaCl BioXtra, Sigma Aldrich) medium supplemented with Amp100 and 1% of glucose (Glc1% ...
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were incubated O/N at 4°C followed by two washing steps of 5 minutes with Wash Buffer A (Sigma) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (f-MLP) (F3506) and prostaglandin E1 (PGE1) were all purchased from Sigma Aldrich. The purinergic receptor agonist ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Plant Biology 2023Quote: ... flowers were treated for three consecutive days one week after bolting with the solution containing 30 μM 1-N-naphthylphthalamic acid (Sigma) and 0.010 % (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... At this point the hct was lowered to 2.5% and the culture was treated for 48–72 h with 50 mM N-acetylglucosamine (NAG; Sigma-Aldrich) in order to clear residual asexual parasites and obtain a virtually pure gametocyte culture ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 mM Na-pyro-phosphate in PBS) supplemented with 10 mM N-ethylmaleimide and Complete EDTA-free protease inhibitor cocktails (Sigma). Lysates were centrifuged at 14,000 rpm for 20 min at 4°C and cleared with protein G-sepharose beads ...
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bioRxiv - Biochemistry 2024Quote: ... cells were sorted into wells containing 1μL of master mix (0.2% n-dodecyl-beta-maltoside (D4641-500MG, Sigma Aldrich, Germany), 100mM tetraethylammonium bicarbonate (17902-500ML ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Neuroscience 2024Quote: RNA extraction was done from age-matched adult Drosophila heads (n=60) using Trizol (Cat no. T9424, Sigma-Aldrich, USA) reagent as per manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Gedunin (Gaia Chemicals, USA) and the specific HSP90 inhibitor 17-AAG (17-N- allylamino-17-demethoxygeldanamycin, Tanespimycin, Sigma-Aldrich, USA) were dissolved in DMSO (dimethyl sulfoxide ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids used to produce ActRII and ActRIIB in bacteria were constructed by inserting the corresponding coding sequence downstream of the N-terminal His6 tag and thrombin cleavage site in pET15b (EMD-Millipore). The plasmid used to produce BMPRII extracellular domain in mammalian cells was constructed by inserting the coding sequence for this downstream of the rat serum albumin signal peptide ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminal peptide enrichment was performed on the digested sample by removing the internal tryptic peptides with undecanal (Sigma, U2202). Enriched N-terminal peptides were then desalted with a C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... diametrically-halved constructs (n=3) were stained over the exposed cross section with a speckled pattern of 1% hematoxylin (Sigma) via airbrush ...
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bioRxiv - Immunology 2023Quote: The obtained plasma from control and depleted fish (n = 18-32 fish) was diluted 10-folds with endotoxin-free PBS (Millipore) and heated at 70 °C for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Expression of WT and mutant N was assessed by Western blot using a rabbit anti-N antiserum 39 and a mouse monoclonal anti-tubulin antibody (Sigma), revealed by incubation with anti-rabbit and anti-mouse antibodies coupled to HRP (SeraCare).
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bioRxiv - Plant Biology 2022Quote: ... The dried wax residues were derivatized using 200 μL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) (derivatization grade, Sigma-Aldrich)/pyridine mixture (1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... the bath application of 30 μM 5-HT together with 50 μM N-methyl-DL-aspartic acid (NMA, Sigma-Aldrich) was applied to the isolated brains ...
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bioRxiv - Microbiology 2022Quote: ... NSm and the entire pcDNA3.1 vector encoding Gn with an N-terminal SS plus HA tag were amplified by PCR using KOD Hot Start DNA polymerase (Novagen), mixed and then concatenated using the NEBuilder HiFi assembly kit according to the manufacturer’s instructions to yield pSnH-OROV-M ...