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Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The expression of the target construct was confirmed by SDS-PAGE and Western blotting with anti-FLAG-HRP antibody (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... The motoneurones were labelled with an antibody directed against Choline acetyltransferase (goat anti-ChAT, Millipore, catalogue number: AB144P, 1:100). This antibody labelled known cholinergic populations of neurones in the correct locations including large ventral horn neurones (motoneurones) ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... 30 μl of extract was resolved on 10 or 12% SDS-PAGE gels and after transfer the membranes were probed with 1:1000 dilution of anti-HA antibody (Sigma). Imaging of blots was done by Biorad ChemiDoc ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for 10 min before sections were incubated at room temperate overnight in a humidified chamber with primary polyclonal rabbit anti-TH antibody at 1:500 (ab152, Millipore). Primary antibody was washed off sections for 5 min in TBS before sections were incubated with biotinylated goat anti-rabbit secondary antibody at 1:500 at room temperature for 1 hour (BA-1000 ...
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bioRxiv - Neuroscience 2020Quote: ... OFC β-endorphin sections were co-labeled with the primary antibody for goat anti parvalbumin (PV+) (1:1000, SIGMA, SAB2500752), a marker for a cortical interneuron subtype ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-RELN G10 (MAB5364, Millipore 1:2000) or 142 (for human RELN ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 0.1% Tween 20) were performed before incubating the membrane with the anti-FLAG antibody 1:5000 for 1 h (Sigma). Next ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with PBS-Tween 20 0.05% and incubated with peroxidase-conjugated anti-human IgG antibody (Sigma, USA) at a dilution of 1 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 5% skimmed milk in Tris Buffered saline-Tween for 1 h before incubation with primary antibody anti-myc tag monoclonal clone 4A6 (Millipore) diluted to final concentration of 200 ng mL−1 in 3% skimmed milk in TBST for 1h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed 4 x 5 min with TBS and the bound SadP was detected with 1:10000 dilution of anti-His primary antibody (Sigma) and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed three times and incubated with a 1:10,000 dilution of horseradish peroxidase-conjugated anti-rabbit antibodies ((Sigma-Aldrich) for 1-2 h ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked with 5% bovine serum albumin (BSA) and then probed with the following primary antibodies - mouse anti-GluN2A (1:1000, Millipore), rabbit anti-GluN2B (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were washed 3×5 min using 1% BSA in PBS followed by 60 min staining using a 1:2000 dilution of the anti-rabbit Atto647N antibody (Sigma). Samples were washed 3×5 min with PBS and stored in light protected container in +4° C until imaged ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then transferred to fresh 0.3% PBS-Triton and incubated overnight at 4°C with appropriate primary antibodies (1:200-500 dilution of goat anti-ChAT IgG, Millipore, AB114P ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2 ...
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bioRxiv - Neuroscience 2022Quote: ... the labeled SPs or anti-ACE2 antibody were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate.
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bioRxiv - Neuroscience 2022Quote: ... 20 μL of beads were washed with PBS and incubated with 6 μg of anti-FLAG antibody (Sigma-Aldrich, F1804) in PBS + 0.5% BSA at 4°C for 3 h with gentle rocking ...
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bioRxiv - Neuroscience 2022Quote: ... wells were washed three times using H2O 0.05% Tween20 and binding of NMDAR antibodies to the immobilized N1-N2B-Fc was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Blots were detected with the Odyssey infrared imaging system (Li-COR Biosciences) and the following primary antibodies: monoclonal anti-tropomyosin clone TM311 (catalog number T2780, Sigma) and monoclonal anti-beta tubulin clone AA2 (catalog number T4026 ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were moved to a humidified chamber with primary antibody (anti-acetyl-alpha tubulin, clone 6-11B-1, Sigma, MABT868) diluted in PBX (1:250 ...
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bioRxiv - Cancer Biology 2022Quote: ... assays were performed as previously described (Bruno T, Cancer Cell, 2002) by using anti-acetyl-Histone H3 (Lys27) antibody (Millipore). Equal amounts of precleared chromatin were added to antibody-bound Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... we added polyclonal antibodies against the extracellular domain of TrkB (1:50 rabbit anti-TrkB, Millipore, cat no. AB9872, RRID:AB_2236301) together with 20 ng/mL BDNF for 2.5 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for Immunostaining were anti-H3P (phosphorylated serine 10 on histone H3; Millipore; 09-797; 1:1000 dilution [4]), anti-Poly (ADP ...
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bioRxiv - Physiology 2019Quote: ... fiber type IIb) (hybridoma supernates, Developmental Studies Hybridoma Bank) supplemented with rabbit polyclonal anti-laminin antibody (2 μg/ml, Sigma) overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... We performed the CTCF nChIP exactly as described before with the same polyclonal anti-CTCF antibody (Millipore, Cat#07-729) (Tsujimura et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Sheared crosslinked chromatin was immunoprecipitated separately with an anti-AR antibody (3 μg, 17-10489 ChIPAb + androgen receptor Assay Kit, Millipore) or an equal amount of normal IgG (3 μg ...
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bioRxiv - Immunology 2019Quote: ... The labeled B cells were then attached to coverslips that had been coated with 0.25 µg/cm2 of the non-stimulatory M5/114 anti-MHC II monoclonal antibody (Millipore, #MABF33). SPT was carried out at 37°C using an Olympus total internal reflection fluorescence microscopy (TIRFM ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS before their incubation with an anti-HA mouse monoclonal antibody (Sigma) for 2 hours at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Monoubiquitin forms of 8.5 kDa were detected using a mouse monoclonal anti-ubiquitin primary antibody (Sigma, U0508, 1:3000 dilution) and a peroxidase-conjugated AffiniPure goat anti-mouse (Jackson Immuno Research ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were spun for 5 seconds in a microfuge and 950 μl of supernatant was transferred to a new tube and incubated with agarose beads-conjugated to anti-Flag antibody (1:100; Sigma) for two hours at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... NGLY1 knock out was confirmed in the cell population by western blotting using the anti-NGLY1 antibody from Sigma (HPA036825). Single GFP negative cells were sorted into a 96 well plate and expanded ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each slide was then incubated overnight at 4° with a 1:100 dilution of mouse monoclonal anti-a-tubulin primary antibody (clone DM1A, Sigma) in blocking buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Developmental Biology 2019Quote: ... the supernatant was transferred into a new tube (and the DNA concentration determined by NanoDrop 2000 Spectrophotometer .25 μg of chromatin were used to immunoprecipitate with 3 μg of the rabbit anti H3K27me3 antibody (Millipore) previously bound to dynabeads overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were then incubated with secondary antibodies for 30 minutes at a dilution of 1:15,000 (Anti-Rabbit IgG 488 (Sigma SAB4600234) and Anti-Mouse IgG 555 (SAB4600068)) ...
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bioRxiv - Microbiology 2019Quote: ... Bound proteins were separated by SDS-PAGE and transferred to nitrocellulose before incubation with an anti-myc antibody (9E10; Sigma). Blots were revealed with the Odyssey Western Detection System.
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bioRxiv - Neuroscience 2019Quote: ... Membranes with transferred proteins were blocked/permeabilized for 1 hour in 5% BSA in TBS and incubated with different antibodies (mouse anti-pMLKL, Millipore, rabbit anti-MLKL ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS and then incubated with 1:50 anti-HA mouse monoclonal antibody (Sigma) in PBS at room temperature for 2 h ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594 - conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...
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bioRxiv - Immunology 2019Quote: ... 50μl of the post-nuclear supernatants was saved as the Input lysate and 450μl were incubated with either 18μg of anti-Flag antibody (Sigma-Aldrich, F7425) or 4.275μg of anti-ZAP antibody (Abcam ...
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bioRxiv - Genomics 2019Quote: ... we blocked coverslips with 5% BSA in PBT for 1 hour at room temperature and then incubated coverslips with properly diluted primary antibody (mouse M2 monoclonal ANTI FLAG, 1:800, F1804, Sigma; rabbit polyclonal Tom20 ...
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bioRxiv - Physiology 2019Quote: ... IHC was performed by incubating sections (n=5 per each group) with the primary antibody (anti-mUCP-1, U6382, Sigma), followed by a secondary antibody conjugated with HRP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were blocked for 30 min in 4% Bovine Serum Albumin then incubated overnight with a mouse anti-puromycin monoclonal antibody (Millipore) (1:1000 in 4% BSA) ...