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Citations for Millipore Sigma :
6801 - 6850 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1% Triton X-100,140 mM NaCl and 0.1% SDS] supplemented with 50 μl β-mercaptoethanol and 1 mM PMSF (Sigma-Aldrich, USA) per ml of RIPA buffer ...
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Neuroscience 2023Quote: ... (AGP-001 and ACC-003, Alomone), blocking peptide for Anti-CaV1.2 (CACNA1C) (BLP-CC003, Alomone) and β-ACTIN (A1978, Sigma-Aldrich). Secondary antibodies used for the immunoblots were Mouse-HRP (115-035-003 ...
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bioRxiv - Cell Biology 2023Quote: ... Receptor-mediated endocytosis was monitored in the proximal tubule of mouse kidneys PTs by measuring the uptake of β-lactoglobulin (L3908, Sigma). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the recombinant proteins was induced by 1 mM isopropyl β-D-thiogalactopyranoside at 37 °;C for 5 h in host Escherichia coli strain Rosetta (DE3) pLysS cells (Novagen). After induction ...
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bioRxiv - Neuroscience 2023Quote: ... were treated to prevent proliferation with cytosine β-D-arabinofuranoside (ara-c; C1768, 2.5 µM, Sigma-Aldrich, St. Louis, MO, USA) and frozen in liquid nitrogen until plated in co-culture with neurons ...
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Single-cell transcription mapping of murine and human mammary organoids responses to female hormonesbioRxiv - Developmental Biology 2023Quote: ... Organoid conditions with “low” levels of estrogen were grown with media supplemented with 33.3 ng/mL of 17-β-Estradiol (Sigma #E2758), and those with “high” levels of estrogen were grown in the presence of 66.6 ng/mL of 17-β-Estradiol ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were diluted in 1X Laemmli sample buffer and heated at 95°C for 10 min in the presence of β-mercaptoethanol (Sigma) (except for OXPHOS cocktail antibody according to manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell suspensions were mixed with 350 µl of freshly prepared lysis buffer (2M NaOH, 7% β-mercaptoethanol) and 350µl of 50% TCA (Sigma, T9159). After spin ...
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bioRxiv - Physiology 2024Quote: ... were spread onto NGM plates containing 100 μg/ml ampicillin and 1 mM isopropyl 1-thio-β-Dgalactopyranoside (IPTG, 420322, Millipore) for 24 hrs ...
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bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with primary antibodies (anti-PYK2 #3292; Cell Signaling Technology, anti-FAK 610088; BD Biosciences, anti-β-actin A5441; Sigma-Aldrich) overnight at 4°C and then with secondary antibodies (LiCOR ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759, 40 mM sodium phosphate, Sigma S5011 ...
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bioRxiv - Cell Biology 2023Quote: ... in PBST (1x PBS, 0.1% Tween20), incubated in β-tubulin (1:1000, CST 2146S) or actin C4 (1:1000, Sigma MAB1501) diluted in 1% milk with 1% BSA in PBST overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Rabbit α-mouse Calmodulin (5μg mL-1, CellSignaling), rabbit α-mouse β-actin (1μg mL-1, CellSignaling) and mouse α-mouse GAPDH (1μg mL-1, Merck/Millipore), goat-α-mouse IRDye 680RD ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was blocked with 1% BSA with 0.05% Tween-20 and reacted with an anti-tau antibody (RTM38, 1:2500) and anti-β-actin antibody (1:10,000; Sigma-Aldrich) at RT for 1 h (or 4°C O/N) ...
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bioRxiv - Biochemistry 2024Quote: Reaction mixes were prepared with the desired ⍺β-tubulin concentrations (7 μM or 10 μM) containing 5% Alexa 568-tubulin plus 1 mg/ml BSA (Sigma) in assay buffer (BRB80 with 30 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (EMD Millipore Corp.; Cas: 367-93-1). Cells were then incubated for 18 to 20 hours at 20°C at 200 rpm ...
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bioRxiv - Genetics 2024Quote: ... Laemmli Buffer (100 mM Tris pH 6.8, 20% glycerol, and 4% SDS (Carl Roth, Germany)) and 5% β-mercaptoethanol (Sigma-Aldrich) were added to the samples after which they were heated to 95°C for 10 minutes.
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bioRxiv - Plant Biology 2020Quote: ... The PCR conditions were set up to amplify only the short 670 bp product using REDExtract-N-Amp PCR ReadyMix (Sigma). PCR conditions ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing N-cTnC were pooled and concentrated using Amicon centrifugal concentrators with a 3 KDa molecular weight cut-off (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... 25 mM HEPES pH 7.4, 1 mM EGTA and 30% v/v Percoll, cat n. GE17-0891-01, Sigma-Aldrich) in a 10-ml polycarbonate ultracentrifuge tube ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the resulting dried extracts were derivatized using methyoxyamine hydrochloride (MOX) and N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) [both purchased from Sigma] ...
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
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bioRxiv - Cancer Biology 2020Quote: ... Coding regions of wildtype BAG2 and ZNF451-BAG2 were amplified from SK-N-AS cells for subcloning into p3XFLAG-CMV14 (Sigma) and pLVX-EF1α-IRES-mCherry (Clontech ...
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bioRxiv - Immunology 2022Quote: ... the DNA sequence for the synthetic N gene (Met1 to Ala 419) was amplified and cloned into the NdeI and XhoI digested pET24a(+) vector (Novagen).
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bioRxiv - Developmental Biology 2019Quote: ... Villin-CreERT2 duodenum (n=4 per genotype) were treated two days after seeding with 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) 1.25 µM for 15h ...
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bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
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bioRxiv - Biochemistry 2019Quote: ... Xa factor site and StreptagII sequence in its N-terminus are digested by HindIII and BamHI and then inserted into a pET-20b plasmid (Novagen). The over-expression in BL21 (DE3 ...
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bioRxiv - Biochemistry 2019Quote: ... Xa cleavage site and StrepTagII sequence in its N-terminus part were digested with XbaI and Hind III enzymes and inserted into pET-20b plasmid (Novagen). The over-expression in Rosetta (DE3 ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... the 1.01 µm diameter carboxylated silica microspheres (SC04000, Bangs Beads) were activated by amine-reactive crosslinker chemistry using NHS (N-hydroxysuccinimide, 130672-5G, Sigma) and functionalized by gelsolin (HPG6-A ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS and 50mM Tris pH 8.0) supplemented with 10 mM freshly prepared N-ethylmaleimide and protease inhibitors (Sigma-Aldrich). Washed GFP-Trap A beads (Chromotek ...
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bioRxiv - Microbiology 2019Quote: ... patient sera were pre-incubated for 10mins with anti-histone single-chain variable fragment (ahscFv; 200 μg/ml, synthesis described previously (18)) or with non-anticoagulant N-acetyl heparin (200 μg/ml; Sigma).
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bioRxiv - Cancer Biology 2019Quote: PyMT [FVB/N-Tg(MMTV-PyVT)634Mul/J] transgenic animals harboring breast tumors were treated with 4-hydroxytamoxifen (Sigma-Aldrich) (1.2 mg/kg/daily ...
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bioRxiv - Biochemistry 2020Quote: ... Fl-SNED1 and the N-terminal fragment were purified by affinity chromatography on an anti-FLAG resin (Sigma-Aldrich, A2220) as previously described (58 ...
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bioRxiv - Plant Biology 2021Quote: ... The released N-glycans were purified on graphitized carbon SPE columns (Supelclean™ ENVI-Carb™, Sigma-Aldrich, Poznań, Poland) and labeled with a fluorescent tag (anthranilic acid ...
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bioRxiv - Plant Biology 2020Quote: ... the pellet was resuspended in minimal volume of 50 mM MOPS (pH 7.5) before being digested with Asp-N endoproteinase (Sigma-Aldrich) at 1:50 (enzyme:acyl-ACP ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in MDCK medium and after inoculation in infection medium containing N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (3µg/mL; Sigma, T1426).
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...