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Citations for Millipore Sigma :
451 - 500 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... After purification with PCR Cleanup kit (Sigma-Aldrich), nucleic acids were eluted in 100 μL of elution buffer (5 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: The KAPA Taq PCR Kit (Sigma-Aldrich KR0352_S) was used to amplify the reads from a cDNA mixture of uninfected samples and 1 ...
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bioRxiv - Microbiology 2024Quote: ... purified (GenElute PCR Clean-up kit, Sigma Aldrich) and quantified using a Qubit Fluorometer (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then transfected with 50 nmol/L miR-6883 mimic (Sigma-Aldrich (HMI2616) using Lipofectamine® RNAiMAX Reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... Not hybridized padlock probes were removed by washes with 10% Formamide in 2X SSC (Sigma-Aldrich, S6639). To minimize the effect of the previously documented SplintR ligase nucleotide preferences (26 ...
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bioRxiv - Cell Biology 2020Quote: ... fixed cells were washed with PBS and hybridization buffer (15% formamide from Sigma-Aldrich in 1X SSC), and then incubated overnight at 37°C in the hybridization buffer also containing 130 ng of the probe set for 100 μl of final volume ...
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bioRxiv - Cell Biology 2022Quote: ... The ovaries were then washed twice in HYBEC wash solution (2x SSC, 15% ethylene carbonate (Sigma Aldrich), 1mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was rinsed three times with 1X DEPC PBS and washed in 0.2X SSCT [0.2X SSC (Quality Biological) and 0.1% Triton X-100 (Sigma) in DEPC water (1:1000 DEPC in ultrapure water ...
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bioRxiv - Molecular Biology 2019Quote: ... at 1200J/m and subsequently blocked in hybridisation buffer (5X SSC, 5% Dextran sulphate (Sigma-Aldrich, D8906) 0.2% Tropix I-Block (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... Dead cells and doublets were excluded on the basis of FSC and SSC distribution and DAPI (Sigma) exclusion ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were rehydrated in RNAse-free 1x SSC buffer containing 15% (v/v) formamide (Sigma-Aldrich®) for 15 minutes and then hybridization solution was applied to coverslips overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with 0.5 µg/ml Hoechst 33258 in 2x saline sodium citrate (SSC) (Sigma) for 15 min and exposed to UV for 30 min in 2x SSC before being treated with exonuclease III (3 U/µl ...
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bioRxiv - Biochemistry 2023Quote: ... 000 total cell events were gated with FSC/SSC gate on a Guava Easycyte 10HT instrument (Millipore). A second gate for high mCherry transfection efficiency was applied ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were washed with 2×20 min SSC-T and incubated in 20 μM Hoechst (Sigma, 2261) diluted in 1×PBS at room temperature in dark for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were then incubated in pre-hybridization buffer [1X SSC solution containing 5% Dextran sulfate (Sigma, D8906), 10mg/ml ultrapure BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue slides were washed twice in Stringent Wash Buffer (2× saline-sodium citrate (SSC) (Millipore Sigma, S6639) in 50% formamide (Millipore Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The blots were washed again in TBST three times for 5 mins each at RT and then exposed to Immobilon Crescendo HRP (Millipore Sigma, Cat # WBLUR0100) for 3 mins at RT and imaged in a Bio-Rad ChemiDoc XRS+ with ImageLab software.
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bioRxiv - Neuroscience 2022Quote: ... the tissue sections pre-stained with DiIC18 for the indicated time were incubated for 30 min at RT with 100 µg/ml digitonin (Sigma Aldrich; cat. D141) dissolved in 3% bovine serum albumin (BSA)/PB ...
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bioRxiv - Genetics 2022Quote: ... DIG detection was carried out using the DIG Luminescent Detection system (Sigma) with CPSD substrate according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and then used for the detection of glucose consumption and lactate production using the Glucose Uptake Colorimetric Assay Kit and Lactate Assay Kit (Sigma) in agreement with the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... Fetal tails were kept for sex determination by detection of the Sry gene using the Taq Ready PCR system (Sigma), specific primers (Sry ...
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bioRxiv - Molecular Biology 2020Quote: Alkaline phosphatase (AP) staining was conducted with Alkaline Phosphatase Detection Kit (SCR004, Millipore. Burlington, MA) as described in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: TUNEL staining was carried out using ApopTag ® Red In Situ Apoptosis Detection Kit (Millipore). Embryos were fixed in 4% paraformaldehyde and dehydrated in 100% methanol for at least 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: TUNEL staining was performed with an ApopTag fluorescein in situ apoptosis detection kit (EMD Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The cell lines were routinely screened using VenorTM GeM Mycoplasma Detection Kit (Sigma-Aldrich, #MP0025) following the manufacture’s protocol.
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... DuolinkTM In Situ PLA Mouse/Rabbit kit with red detection reagents (Sigma-Aldrich, DUO92101-1KT) was used according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Apoptosis was determined with the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7110) (2017-2019) ...
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bioRxiv - Pathology 2021Quote: ... OxyIHC staining was performed according the Millipore OxyIHCTM oxidative stress detection kit protocol (Millipore, S7450).
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bioRxiv - Genetics 2019Quote: ... TUNEL assays were performed using an ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore, S7100). Thymus sections were used as positive controls ...
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bioRxiv - Systems Biology 2021Quote: ... Apoptosis was assessed in dissociated spheroids using Annexin V-FITC Apoptosis Detection Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Dying cells were visualized with the ApopTag® Red In Situ Apoptosis Detection kit (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... TUNEL staining was performed using In Situ Cell Death Detection Fluorecein kit (Sigma cat# 11684795910) prior to addition of anti-recoverin primary antibody ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: PLA was performed using the Duolink Detection Reagents Brightfield Kit (Sigma-Aldrich, Cat. No. DUO92012) and associated wash buffers (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... BACE1 activity was measured using the β-Secretase (BACE1) Activity Detection Kit (Fluorescent) (Sigma: CS0010). Equal amounts of lysate were added to each well and run in duplicate ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was recovered and used with the protease fluorescent detection kit (PF0100 – Sigma-Aldrich). The protocol was adjusted from the manufacturers’ instructions in the following ways ...
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bioRxiv - Neuroscience 2023Quote: ... PLA signals were detected using the red Duolink in situ detection reagent kit (DUO92008, Sigma) and imaged on the Zeiss LSM700 ...
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bioRxiv - Synthetic Biology 2023Quote: ... This assay was performed according to BACE1 Activity Detection Kit protocols (Sigma, St. Louis, MO). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: PLA was performed using the Duolink in situ detection reagents far-red kit (Sigma, DUO92013), PLA-probe anti-mouse PLUS (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... DuolinkTM In Situ PLA Mouse/Rabbit kit with red detection reagents (Sigma-Aldrich, DUO92101-1KT) was used according to manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore; Watford, UK). The following antibodies were used ...
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bioRxiv - Immunology 2023Quote: ... they were detected for intermolecular binding using Duolink flow PLA detection kit (Green) (Sigma/Merck) with a reaction time of 100 minutes for post-mouse/rabbit probe linking amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescent signal was generated using Duolink In Situ Detection Reagents Red kit (Sigma-Aldrich, DUO92008). Cells were mounted and the nuclei were stained using the Duolink In Situ Mounting Medium with DAPI (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... an in situ detection kit was used according to the manufacturer’s protocol (Sigma-Aldrich, USA). Sample pretreatment with DNase I served as positive control and TUNEL reaction mixture without terminal transferase (TdT ...
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bioRxiv - Bioengineering 2023Quote: ... ApopTag® Peroxidase In Situ Apoptosis Detection Kit was purchased from Millipore (Billerica, MA, USA).
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bioRxiv - Cell Biology 2024Quote: ... Cultured cells and parasites were screened against mycoplasma contamination with LookOut Mycoplasma detection kit (Sigma) and Mycoplasma Removal Agent (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... aggregated and nitrated hASYN signals were detected using a brightfield detection kit (Duolink, Sigma-Aldrich). Fluorescence detection of nitrated NDUFB8 was achieved using a Duolink Red Detection Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Developmental Biology 2022Quote: ... Several probes were produced by PCR amplification (KAPA2G Fast ReadyMix PCR kit, Sigma Israel, KK5101). cDNA templates were synthesized by RNA precipitation followed by reverse transcription PCR ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...