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Citations for Millipore Sigma :
451 - 500 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... wells were washed in PBS 3x 5minutes and subsequently incubated with primary antibodies either 1h RT or over night at 4°C in antibody mix solution pH7.4 (for 100ml: 10mL 1x triton, 5mL Tris-HCl pH7.5, 0.9grNaCl, 0.25gr porcine gelatin (G8150, Sigma) and sterile water) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in hypotonic lysis buffer (50 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 0.1% IGEPAL CA-630 (Sigma), 10% glycerol ...
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bioRxiv - Genomics 2022Quote: ... both diluted in 1× TNB (1M Tris HCl pH 7.5, 3 M NaCl-blocking reagent, Sigma-Aldrich) with posterior incubation for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... All glassware was cleaned with 1% (0.33 M) hydrochloric acid (HCl, 32%; Fluka, Sigma-Aldrich, Buchs, Switzerland), rinsed with nanopure water ...
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bioRxiv - Bioengineering 2019Quote: ... and fibrinogen (FBG; from human plasma, 20 mM sodium citrate-HCl pH 7.4, Lot #3169957, Millipore Sigma). FAM-proteins were prepared with 10 mg of protein reconstituted in 900 μL of 0.1 M PBS and 8-fold molar excess of FAM NHS ester solubilized in 100 μL DMSO ...
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bioRxiv - Bioengineering 2019Quote: ... sputum aliquots were diluted 10-fold the original volume in 8 M guanidine-HCl (Sigma Aldrich, MO). Samples were pretreated with 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then treated with a hypotonic solution (30 mM Tris-HCl, 50 mM sucrose (Sigma, S0389), 17 mM trisodium citrate ...
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bioRxiv - Bioengineering 2020Quote: ... digestates were hydrolyzed for 1 hour at 121°C and 15 psi in 6N HCl (Sigma-Aldrich). Hydrolyzed digestates then underwent hydroxyproline assay as described by Cissell et al,(35 ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining pellets were mixed with 20μl of a lysis buffer (60 mM Tris-HCL (Sigma, 10812846001) pH 7,2% SDS and ...
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bioRxiv - Cell Biology 2021Quote: ... The DAB substrate mix was freshly prepared using 3.5 mM DAB (3,3’-Diaminobenzidine tetra–HCl, obtained from TAAB UK) and 0.02% H2O2 (Sigma) in 50 mM Tris-HCl at a pH of 7.6 ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized samples were first derivatized in 10 μL of 20 mg/mL methoxyamine HCl (Sigma, Cat#: 226904) dissolved in pyridine (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... was then mixed 1:1 with 2x synthetic gastric fluid (SG) according to the US Pharmacopoeia at 0.01N HCl (4.0 g NaCl and 6.4 g pepsin (Sigma) dissolved in 14 ml of 1N HCl ...
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bioRxiv - Microbiology 2020Quote: At least 250,000 cells were lysed in buffer containing 50 mM Tris-HCl (pH 7.8; Sigma-Aldrich), 100 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... and imaged in imaging buffer (50 mM Tris-HCl, 10% glucose,1% 2-Mercapgtoethanol (Sigma-Aldrich M6250), 50 U/mL glucose oxidase (Sigma Aldrich G2133-10KU) ...
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bioRxiv - Molecular Biology 2022Quote: ... approximately 1 x 106 cells were incubated in 250 µl of ice-cold Hi-C lysis buffer (10mM Tris-HCl pH8.0, 10mM NaCl, 0.2% Igepal CA630) with 50µl of protease inhibitors (Sigma) on ice for 30 minutes and washed with 250 µl lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... DNA pellets were resuspended in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8, Sigma) and stored at -20 °C until used for sequencing.
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bioRxiv - Physiology 2020Quote: ... we add 0.01 N hydrochloric acid (HCl) or 25 µg/ml porcine gastric mucin (M2378, Sigma Aldrich), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted either in 100 μl 3 M guanidinium hy-drochloride (Gnd-HCl, Sigma-Aldrich, G3272) in 0.1 M Tris ...
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bioRxiv - Biophysics 2021Quote: ... 10mM tris HCL pH 7,4 was used to diluted the 60% iodixanol stock solution (OptiPrep from Sigma) and to prepare 40% and 20% iodixanol solution ...
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bioRxiv - Bioengineering 2021Quote: ... in TBST (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.05 % v/v Tween-20, Sigma) for 30 min at RT ...
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bioRxiv - Genomics 2019Quote: ... Cell pellets were resuspended in 250 μl nuclei permeabilization buffer (10 mM Tris-HCl pH 7.4 (Sigma), 10 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mM EDTA and 50 mM Tris-HCl pH 8.1) containing COmplete EDTA free protease inhibitors (Sigma). The cell lysates were sonicated with a Bioruptor Plus (Diagenode ...
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bioRxiv - Neuroscience 2019Quote: ... separate cohorts of pups received intraperitoneal injections of the corticosterone inhibitor metyrapone HCL (50 mg/kg, Sigma) or an equal volume of 0.9% saline ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized samples were first derivatized in 10 μL of 20 mg/mL methoxyamine HCl (Sigma, Cat#: 226904) dissolved in pyridine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were eluted in 50 mM Tris-HCl (pH 8) containing 10 mM reduced glutathione (Sigma). Glutathione was removed using a desalting Zeba column following manufacturer instructions (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... dried overnight at room temperature and re-suspended in 10 mM Tris-HCl pH 8.0 (Sigma T6066).
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bioRxiv - Neuroscience 2021Quote: ... and 10% (w/v) glucose) dissolved in a buffer composed of 100 mM Tris-HCl (Sigma-Aldrich), 1 mM ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 20mM Tris-Hcl pH 8.0, 5mM EDTA, 10M Na2HPO4 pH 7.2, 10% dextran sulfate, 1X denhardt’s solution [Sigma D2532] ...
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bioRxiv - Microbiology 2020Quote: ... 0,25 mL of concentrated and normalized supernatant was added to 0,75 mL reaction buffer (0,05M Tris-HCl, 0,5 mM CaCl2, pH 7,5, 3 mg.mL-1 Azocasein (Sigma A2765)) preheated at 37°C and vortexed ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen telencephalon tissue sample pools were transferred into chilled lysis buffer containing 10 mM Tris-HCL (Sigma), 10 mM NaCl (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were pretreated with Tris-HCl and proteinase K (20 mg/mL, Sigma-Aldrich, Taufkirchen, Germany). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl pH 7.5, 2.5 mM EDTA, 140 mM NaCl, 0.7% Protease Inhibitor Cocktail II [Sigma] ...
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bioRxiv - Microbiology 2023Quote: ... The cells were either directly lysed in 750 μl of the mild lysis buffer (0.1M Tris-HCl pH 7.8 with 0.5% Triton X100 supplemented with a protease inhibitor cocktail (Millipore-Sigma)) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mM Tris-HCl (pH7.4)] with ATP detection system containing 5 mM α-ketoglutarate (Sigma-Aldrich 75892), 0.01 mM Ap5A (Sigma D6392) ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked for 1 hour at room temperature in TTBS buffer (20 mM Tris-HCl [pH 7.6], 137 mM NaCl (1.06404.1000, Merck Millipore), and 0.25% Tween (8.22184.0500 ...
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bioRxiv - Genetics 2023Quote: ... cells were resuspended in 100µL ice-cold Lysis Buffer (10 mM Tris-HCl pH 7.4 (Millipore-Sigma), 10 mM NaCl (Ambion/Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... cells were resuspended in 100µL ice-cold Lysis Buffer (10 mM Tris-HCl pH 7.4 (Millipore-Sigma), 10 mM NaCl (Ambion/Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... cells were resuspended in 100µL ice-cold Lysis Buffer (10 mM Tris-HCl pH 7.4 (Millipore-Sigma), 10 mM NaCl (Ambion/Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... cells were resuspended in 100µL ice-cold Lysis Buffer (10 mM Tris-HCl pH 7.4 (Millipore-Sigma), 10 mM NaCl (Ambion/Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... formazan was solubilized by adding 2-propanol 0.1 M HCl (Sigma-Aldrich, Merck Life Science, Milan, Italy). The absorbance was measured at wavelength 570 nm (660 nm background wavelength ...
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bioRxiv - Physiology 2023Quote: ... 5X SDS sample buffer (250 µM Tris/HCl, pH 7.0; 40% (v/v) glycerol (Sigma-Aldrich #G5516); 8% (w/v ...
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bioRxiv - Bioengineering 2023Quote: Precultures were grown in buffered SD media supplemented with 2000 µg/L thiamine (thiamine HCL, Sigma-Aldrich) for 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... washed and incubated in Tris buffer 0.05M (20 mM Tris-HCl and 150 mM NaCl, Sigma-Aldrich, Cat ...
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bioRxiv - Neuroscience 2023Quote: Dorsal cortices were lysed in RIPA buffer (0.05M Tris-HCl, pH 7.4, 0.5M NaCl, 0.25% deoxycholic acid, 1% Triton X-100, and 1 mM EDTA, Millipore) and supplemented with 0.1% SDS ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 10 mg of the sample was mixed with 1 mL of 6 N HCl (Sigma, USA) in a 2 mL screw-cap tube ...
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bioRxiv - Neuroscience 2023Quote: ... Pooled samples were then incubated in 150 μl chilled lysis buffer containing 10 mM Tris- HCL (Sigma), 10 mM NACl (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... and eluted with GST elution-buffer (50 mM Tris-HCl [pH 8.0], 10 mM reduced glutathione (Sigma)) as described previously51.
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bioRxiv - Genomics 2023Quote: ... in a buffer consisting of 50 mM Tris HCl (pH 8.0, Millipore Sigma, Product No. T2694-100ML), 1 mM MgCl2 (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: All cell pellets were lysed using a buffer containing 50 mM Tris-HCl (Trizma Base: Sigma-Aldrich, Cat#T6066 ...
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bioRxiv - Genetics 2024Quote: ... were lysed with 175 μl of cold cytoRNA solution (50 mM Tris HCl pH 8.0; 140 mM NaCl; 1.5 mM MgCl2; 0,5% NP-40; 2mM Vanadyl Ribonucleoside Complex; Sigma) and incubated 5′ in ice ...