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Citations for Millipore Sigma :
6701 - 6750 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Full length Xenopus laevis Polκ with an N-terminal 6xHis-tag was amplified from pCMV-Sport.ccdb-Polκ (Williams et al., 2012) and cloned into pET28b (Novagen) using primers A and B ...
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bioRxiv - Microbiology 2023Quote: ... Depending on the culture density up to 1 L of sample was filtered through each (n = 5 per sampling day) 0.22 µm Sterivex polyethersulfone membrane filter unit (Millipore) to obtain biomass for DNA extraction and following amplicon sequencing of 16S and 18S small subunit ribosomal RNA (SSU rRNA ...
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bioRxiv - Microbiology 2023Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre- soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Neuroscience 2023Quote: ... The antennae were bent outwards from the head and stabilized by a small droplet of n-eicosane (Sigma Aldrich) onto the edge of a microscopy glass slide ...
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bioRxiv - Biochemistry 2023Quote: ... Tubulin was further purified by recycling and some tubulin was labeled with Alexa647-N-hydroxysuccinimide ester (NHS; Sigma-Aldrich), Atto647-NHS (Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Sections were incubated o/n (4°C) with primary antibody against Laminin (Rabbit polyclonal, Sigma, L9393, diluted 1:20). Alexa Fluor Donkey anti-Rabbit 488 (A11034 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... reactions were diluted in 500 µl immune-precipitation buffer (RIPA buffer supplemented with 40 mM N-Ethylmaleimide (Sigma-Aldrich), 40 mM iodoacetamide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA (10 µg) was separated by denaturing agarose gel electrophoresis and transferred onto Hybon-N+ membrane (Sigma-Aldrich) and probed with [32P]dCTP-radiolabelled DNA fragments ...
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bioRxiv - Microbiology 2024Quote: ... along with 25 μg/ml containing complete media 1µM of SIRT1 (EX-527) inhibitor or SIRT3 (3TYP) or 10mM of N-Acetyl Cysteine (NAC, Sigma) or 50nM of chetomin (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Some vessel segments were incubated with L-NAME (N-nitro-L-arginine methyl ester 100 μmol.L-1, Sigma-Aldrich), The mechanical activity was isometrically recorded using a force transducer and a data acquisition system (DATAQ Instruments ...
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bioRxiv - Biochemistry 2024Quote: ... Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3) cells (Novagen). E.coli cells were grown in TB media up to an OD600 of ∼ 1.2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... one aliquot of culture supernatant was diluted to 24% in Milliplex Assay Buffer and analyzed using a Milliplex MAP Mouse Cytokine/Chemokine Multiplex Assay (Millipore Sigma, Cat No. MCYTOMAG-70K) per manufacturer’s instructions for the following custom collection of analytes ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μL of extract were incubated with 5 μL of β-Glucosidase from almonds (0.5 U/μl in acetate buffer, Sigma-Aldrich) during 90 min at 37°C to release SA from SA-glucoside (SAG) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in LB broth to an OD600 of 0.1 and 5 μl used to inoculate 96-well culture plates containing 150 μl LB agar supplemented with CPRG (Chlorophenol red-β-D-galactopyranoside – Sigma) (20 μg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was either directly added to an equal volume of 2X Laemmli buffer containing 2% β-mercaptoethanol or precipitated with trichloroacetic acid (Sigma) and washed with acetone prior to resuspension in 2X Laemmli containing 2% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... cells were starved in charcoaled stripped medium for 48 h and stimulated for 5 minutes with 100 nM β-estradiol (Sigma) prior to the measurements ...
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bioRxiv - Immunology 2020Quote: MutuDCs were seeded at 150,000 cells/well in U-bottom 96-well plates and incubated with 10 mg/ml β-lactamase (Sigma, #P0389) for 3 h at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were cultured at 37°C overnight and induced by addition of 0.2 mM isopropyl-1-thio-β-d-galactoside (Sigma-Aldrich) for 4 hours at 28°C ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Molecular Biology 2021Quote: ... were from Santa Cruz Biotechnology (Dallas, TX, USA). Loading control β-Actin (Cat No. A2228) antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). ILK antibody was obtained from Elabscience (Houston ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved using SDS-PAGE and transferred to nitrocellulose membranes before analysis with anti-MYC (Cell Signalling) anti-β-Actin (Sigma) antibodies ...
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bioRxiv - Genomics 2020Quote: ... cells were blocked with 1 × PBS solution containing 50 mM Glycine for 10 min twice and then washed once in 1 × PBS before being subjected to dual-color imaging in dSTORM imaging buffer (1 × PBS, 100 mM β-mercaptoethylamine (Sigma), pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in LB broth to an OD600 of 0.1 and 5 μl used to inoculate 96-well culture plates containing 150 μl LB agar supplemented with CPRG (Chlorophenol red-β-D-galactopyranoside – Sigma) (20 μg/ml) ...
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bioRxiv - Cell Biology 2019Quote: ... K124 and K42 immunoblots were reprobed with anti-keratin K14 and mouse anti-β-actin-HRP mAb (K124 blots; 1:15,000, clone AC-15; Sigma-Aldrich) or anti-β-actin-HRP alone (K42 blots ...
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bioRxiv - Molecular Biology 2019Quote: ... then inoculated in required media at 2×104 cells/ml and allowed to recover for 2 hours at 30° before addition of 1μM β-estradiol (Sigma E2758) and CuSO4 as required ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 rabbit anti-β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Immunology 2019Quote: ... Methyl-β-cyclodextrin (MBCD) and water-soluble cholesterol (Xu et al. 2010) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... LMPs were cultured in this cholesterol-free medium for 20 t0 24 hrs and then treated with 10mM methyl-β-cyclodextrin (MβCD, Sigma C4555) for 15min at 37°C to remove cholesterol from plasma membranes ...
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bioRxiv - Biochemistry 2020Quote: ... HLCs were exposed to either 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate (AICAR; 1mM; Sigma-Aldrich, A1393-50MG), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA ...
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bioRxiv - Neuroscience 2020Quote: ... Equal concentrations of serially diluted lysate (in the range of 0-60 μg/ml) from control and Thap1 cKO genotypes was incubated with 10 mM 4-methylumbelliferyl β-D-glucuronide (MUG, Sigma) in 0.2M Sodium acetate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were incubated at 37 °C overnight in a 1.5% BSA solution with anti-β-III-tubulin (anti-rabbit, 1:500, Sigma-Aldrich) and rinsed three times with PBS ...
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bioRxiv - Biophysics 2021Quote: ... All experiments were prepared in PBS and contained either 0.2 μM of the respective DegP enzyme (monomer concentration) and 7.5 μM of p23 peptide or 10 μM of the enzyme with 50 μM β-Casein (Sigma-Aldrich) and 100 μM of the Abz-KASPVSLGYNO2D reporter peptide ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections incubated at room temperature overnight in primary antibody medium or control medium (1:100 primary anti β-catenin antibody from rabbit Sigma C2206 + 1:100 primary anti β-actin antibody from mouse Sigma A5441 + PBS pre-incubation medium ...
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bioRxiv - Biochemistry 2021Quote: ... Bacteria were grown to an OD600 of 0.8 in lysogenic broth (LB (Luria/Miller)) medium (Carl Roth) before the expression was induced with 0.5 mM isopropyl-β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich). Following overnight expression at 18°C ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were eluted with 2x Laemmli sample buffer and heated at 95°C for 5 min in the presence of β-mercaptoethanol (Sigma). Whole cell lysates or purified protein samples were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... 50 µL of extract were incubated with 5 µL of β-Glucosidase from almonds (0.5 U/µl in acetate buffer, Sigma-Aldrich) during 90 min at 37°C to release SA from SA-glucoside (SAG) ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted 1:500 and β-actin was used as loading control (1:10,000, Sigma-Aldrich, Cat. No. A5316, RRID: AB_476743). An horseradish peroxidase (HRP)-coupled rabbit anti-mouse antibody (Dako ...
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bioRxiv - Biochemistry 2022Quote: All-trans-astaxanthin and β-carotene (CAS Numbers: 472-61-7 and 7235-40-7) were purchased from Sigma-Aldrich (USA). Lutein was HPLC-purified from a commercial lutein preparation purchased from RealCaps ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... 50% of the medium was replaced with growth medium additionally supplemented with 4 μM cytosine-1-β-D-arabinofuranoside (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... RNA stability was assessed by incubating 0.5-1 × 107 cells with 50 μg/ml of the transcription inhibitor 5,6-Dichlorobenzimidizole 1-β-D-ribofuranoside (DRB) (Sigma Aldrich). Jurkat T cells (1.25-2.5×106 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed 3 times with PBS and mounted on a microscope slide with parafilm gasket in 1% (v/v) β-mercaptoethanol (Sigma), 150 mM Tris ...
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bioRxiv - Biophysics 2020Quote: The U2OS cells for both DNA and RNA Pol II images were treated with 100 μM 5,6 Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB; Sigma-Aldrich) for transcription inhibition prior to live-cell image acquisition ...
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bioRxiv - Neuroscience 2021Quote: ... in TBST for 1 h at room temperature (RT) and incubated with primary antibodies against β-actin (mouse, 1:50,000, Sigma, A5316) and Myosin Light Chain 2 (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... for 10 min before all samples were incubated for 1 hr with 2.5 mM of the fluorescent GCase substrate methylumbillifery β-D-glucopyranoside (4MUG, Sigma-Aldrich) at 37 °C in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... With the fluorescent GCase substrates blue fluorogenic substrate 4-methylumbelliferyl-β-D-glucopyranoside (4-MU) (Sigma-Aldrich; as described in 11,12), PBMCs were washed 2x with PBS and lysed in 60μl of activity assay buffer (50mM Citric acid ...