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Citations for Millipore Sigma :
6651 - 6700 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cells were permeabilized with 0.2% Triton-X100/PBS for 10 min at room temperature and blocked for 1 h in 0.5% BSA (w/v) (Millipore, 126579) in 0.2% Triton-X100/PBS (blocking buffer) ...
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bioRxiv - Plant Biology 2023Quote: ... Seedlings were then transferred to 1.0% (w/v) GM agar plates supplemented with/without indicated concentrations of ABA (Sigma-Aldrich) or sorbitol and grown vertically for an additional 7-11 days ...
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bioRxiv - Microbiology 2023Quote: ... difficile was streak on Brain heart infusion (BHI) agar plus 0.1% (w/v) sodium taurocholate hydrate (Cat. 86339, Sigma-Aldrich). A single colony was picked and subcultured in BHI medium (BD ...
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bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
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bioRxiv - Biophysics 2023Quote: ... Cells were resuspended in 100 µl FACS blocking buffer (phosphate buffered saline (PBS) supplemented with 5% (w/v) bovine serum albumin (BSA) (Sigma)) and centrifuged at 400g for 5 min at 25°C ...
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SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomesbioRxiv - Biochemistry 2022Quote: ... control and gSpns1-cKO mice were injected with 4 μl/g body weight of a 17% (w/v in 0.9% NaCl) Triton WR1339 solution (Sigma Aldrich) for three days prior to fractionation of lysosomes ...
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bioRxiv - Cell Biology 2023Quote: Fresh tumor-free lung tissue was filled with a 3% (w/v) low melting agarose preparation media (Sigma, A9414-100G). Agarose filling was performed via a catheter inserted through one or several identified bronchi ...
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bioRxiv - Bioengineering 2023Quote: ... the dermis construct was consolidated in a solution composed of 3% (w/v) calcium chloride (Calcium Chloride, Sigma Aldrich, C5670) and Thrombin at a concentration of 10 U/mL (Thrombin from bovine plasma ...
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bioRxiv - Cell Biology 2023Quote: ... The post-fixed tissues were then submerged in graded concentration (10-20-30% (w/v)) of sucrose (S9378, SIGMA Aldrich) in PBS at 4°C until the tissue sunk in each solution to cryoprotect the samples ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed with PBS pH 7.4 and blocked with 5% (w/v) bovine serum albumin (BSA) (Sigma Aldrich, #A9648) for 2 h at 4 °C and finally stained with anti-8-oxo-dG (Trevigen ...
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bioRxiv - Immunology 2023Quote: ... BX471 was dissolved at a final concentration of 10 mg/ml in saline containing 40% (w/v) cyclodextrin (Sigma-Aldrich); the solution was mixed thoroughly and dissolved overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... was removed by incubating the embryos in pronase (0.5% w/v Proteinase K in H-KSOMaa supplemented with 0.5% PVP-40 (Sigma-Aldrich, P0930)) covered with mineral oil for 2-3 minutes ...
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bioRxiv - Immunology 2023Quote: ... sensitized mice were challenged intravenously (i.v.) through the retro-orbital sinus with 5 mg/kg of grade V OVA (A5503, Sigma-Aldrich) 14 days after the first sensitization at ZT4 ...
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bioRxiv - Microbiology 2023Quote: ... before staining with a 0.1 % (w/v) solution of the DNA-binding dye 4,6-diamidino-2-phenylindole (DAPI) (Sigma, Deisenhofen, Germany) at room temperature for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rehydrated and stained with a 0.25% w/v thionine solution (sold as “thionin acetate” by Sigma-Aldrich; MW 287.34 g/mol ...
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bioRxiv - Microbiology 2023Quote: ... and blocked for 1 hour in PBS containing 0.1% w/v saponin-3% bovine serum albumin (BSA, Sigma Aldrich, A7906). Samples were again rinsed three times with PBS and incubated with primary antibodies as indicated in Table S5D ...
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bioRxiv - Developmental Biology 2023Quote: ... explants were washed in serum-free IMDM with 0.1% (w/v) bovine serum albumin (BSA, Sigma-Aldrich; St. Louis MO) and incubated for 2-24hrs at 37°C 5% CO2 +/- myristoylated aPKC pseudosubstrate inhibitor (5 μM ...
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bioRxiv - Biophysics 2023Quote: ... different amounts of freeze dried GelMA macromer were dissolved in DPBS containing 0.5% (w/v) 2-Hydroxy-4’-(2-hydroxyethoxy)-2- methylpropiophenone (Sigma-Aldrich) as photoinitiator ...
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bioRxiv - Plant Biology 2023Quote: Leaf disks (3 mm) of Arabidopsis thaliana and Nicotiana benthamiana were fixed with 3% (w/v) glutaraldehyde (Sigma, Taufkirchen, Germany) in 0.1 M sodium cacodylate buffer (SCB ...
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bioRxiv - Developmental Biology 2023Quote: ... was removed from blastocysts with pronase (0.5% w/v Proteinase K, Sigma P8811, in global® medium containing HEPES supplemented with 0.5% PVP-40, Sigma, P0930) treatment for 2-3 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... or for cocultures 50μl of degrader and 50µl of cross-feeder cultures were mixed and spotted on plates that were made using TR medium containing 0.1% (w/v) alginate (Sigma Aldrich) and 1% agarose (Applichem) ...
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bioRxiv - Plant Biology 2023Quote: ... clarus were incubated for 5 min on 350 μL of 0.4% (w/v) Phytagel (Sigma-Aldrich, St Louis, MO, USA) containing M medium (Hildebrandt et al. ...
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bioRxiv - Neuroscience 2023Quote: ... mice were then perfused with prewarmed (37°C) FITC-albumin gel [2.5% w/v gelatin (Sigma-Aldrich, cat. #G1890-1KG), 0.05% w/v FITC-albumin (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... Dried and frozen metabolite extracts were derivatized with 50 µl of 2% (w/v) hydroxylamine hydrochloride in pyridine (Millipore Sigma) for 60 min at 90°C ...
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bioRxiv - Plant Biology 2023Quote: ... Roots were incubated at 37°C for 135 minutes in an enzyme mixture containing 5% (w/v) pectinase from Aspergillus niger (Sigma), 2% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The scattered light intensity of the column eluant was recorded at 18 different angles using a DAWN-HELEOS MALS detector (Wyatt Technology Corp.) operating at 658 nm after calibration with the monomer fraction of Type V BSA (Sigma). Protein concentration of the eluant was determined using an in-line Optilab T-rex interferometric refractometer (Wyatt Technology Corp.) ...
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bioRxiv - Plant Biology 2024Quote: ... samples were processed the same way except that membranes were blocked in 1% (w/v) BSA in TBS-T and incubated in 1:1,000 streptavidin-HRP (Sigma, S2438).
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bioRxiv - Cancer Biology 2024Quote: ... Colonies are allowed to grow for ten days and then fixed and stained with 0.5% (w/v) Crystal Violet (Sigma-Aldrich) in 20% methanol ...
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bioRxiv - Immunology 2023Quote: ... Minced pancreas samples from NOD mice were digested in RPMI-10 supplemented with 5 mg/ml Collagenase Type V (Sigma) and 10 ug/ml DNase I followed by incubation in enzyme-free cell dissociation buffer (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... PdgfrβCre-tdTomato mice were injected with 25 µL of 5% (w/v in saline) 70kDa FITC-dextran (Sigma-Aldrich; 46945), PdgfrβCre-tdTomato ...
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bioRxiv - Bioengineering 2023Quote: ... yeast cultures were grown in synthetic complete media containing 0.67% (w/v) Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich), 1.92 g/L Yeast Synthetic Media Dropout Mix (uracil ...
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bioRxiv - Biophysics 2023Quote: ... cerevisiae that were corrected for auxotrophies at all loci were cultured in synthetic defined yeast media (SD-min) made with 2 % (w/v) D-glucose-13C6 (Sigma), 0.225 % (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested in ice-cold 1X PBS and mechanically homogenized in 2ml 8% (w/v) sucrose (0.25M; Sigma, S9378) using glass douncers ...
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bioRxiv - Molecular Biology 2023Quote: EU labelled cells were co-stained with anti-Histone H4 pAb (1:250 in Dulbecco wash buffer with 0.2 % w/v BSA) (SAB4500311; Sigma Aldrich) for 30 mins at room temperature in a dark humidified chamber ...
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bioRxiv - Biophysics 2023Quote: Stock solutions of 2% w/v low gelling-temperature agarose (BioReagent, for molecular biology; Sigma-Aldrich, St Louis, MO, USA) were made by dispersing agarose powder in PBS and microwaving at 350W power in 5-8s intervals until dissolved ...
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bioRxiv - Bioengineering 2023Quote: ... for 15 min and then cell blocking solution (5% w/v BSA, purchased from Millipore Sigma, dissolved in 1x DPBS) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were analyzed using the annexin V and dead cell protocol in the Guava Muse® cell analyzer (Millipore).
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl and 10% (w/v) glucose with an oxygen-scavenging GLOX solution (0.5 mg/ml glucose oxidase (Sigma-Aldrich), 40 µg/ml catalase (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Culture medium was collected 48-and 72-hours post-transfection and 100X concentrated by 50% (w/v) PEG 8000 (P2139, Sigma). Infections were performed using 10 µL virus and 8 µg/ml protamine sulphate in 2 ml culture medium.
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bioRxiv - Systems Biology 2024Quote: ... Sorted single and ten cells were directly collected in 2 µL of lysis buffer (50 mM TEAB, pH 8.5 + 0.025% (w/v) n-dodecyl-β-D-maltoside (DDM) (Sigma-Aldrich)) per well ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Plant Biology 2024Quote: Arabidopsis Ws-2 CCR2::LUC WT seeds were surface sterilized and plated on 1x MS medium containing 3% w/v sucrose (Sigma), 1.5% w/v phytoagar (Duchefa) ...
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bioRxiv - Cell Biology 2024Quote: ... all tubes for a-factor treatments were coated with 1% w/v bovine serum albumin (BSA; Sigma-Aldrich, Waltham, MA) solution ...
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bioRxiv - Plant Biology 2024Quote: ... The leaf was subsequently washed twice with 1x PBS and transferred to ClearSee solution (Xylitol [final concentration 10% (w/v)] (Sigma), sodium deoxycholate [final concentration 15% (w/v)] (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: Following the standard whole-mount immunohistochemistry protocol, embryos were progressed through increasing concentrations of fructose (35%, 40%, 60% and 80% w/v) in 8M urea (Sigma), 0.5% (v/v ...
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bioRxiv - Genomics 2024Quote: ... the coverslips were mounted on glass slides using an antifade mounting media (10% w/v Mowiol 4-88(EMD Millipore)/1% w/v DABCO (Sigma-Aldrich)/25% glycerol/0.1 M Tris ...
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bioRxiv - Cell Biology 2024Quote: ... the chips were incubated with 0.1% (m/v) gelatin combined with 20 µg/ml human plasma fibronectin (Sigma-Aldrich, 341635) in PBS at 37°C for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... the cells were collected from each 12-well plate well and prepared for flow cytometry by staining the surface epitopes in flow staining buffer consisting of 1× DPBS supplemented with 1.0% (w/v) bovine serum albumin (#A2153, Sigma-Aldrich) and 0.020% sodium azide (#S8032 ...
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bioRxiv - Microbiology 2024Quote: ... the cultures were washed with a solution of 0.9% (w/v) NaCl prior to inoculation) and a volume of 60 µl of mineral oil (Sigma Aldrich) was added to each well in order to prevent evaporation of the medium during growth ...
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bioRxiv - Biophysics 2024Quote: ... The glass in the chamber was coated with 70 μl of poly-L-lysine (PLL, 0.01 % w/v, P4707, Sigma-Aldrich) for 30 min ...