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Citations for Millipore Sigma :
6501 - 6550 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Immunology 2021Quote: Kynurenine was measured in cell culture supernatants by incubating the sample with a 30% w/v Trichloroacetic acid (Sigma-Aldrich) solution prepared in water ...
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bioRxiv - Cancer Biology 2020Quote: ... Germany) single 250 V pulse for 10ms with 300mM of MYB siRNA or a scrambled negative control siRNA (SIGMA ALDRICH) as previously reported in Clarke et al (Clarke et al. ...
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bioRxiv - Bioengineering 2020Quote: ... 5) Skin cleaning with saline and application of polyvinylpyrrolidone (PVP-K90, 1% w/v) (Sigma-Aldrich Inch, St. Louis, Missouri); 6 ...
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bioRxiv - Bioengineering 2021Quote: ... a stock solution of 2% w/v agarose was initially generated by mixing the requisite amount of agarose (A9414, Millipore-Sigma) and DI water at 70 °C until dissolved ...
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bioRxiv - Biophysics 2021Quote: ... dishes were coated with a 0.01 % (w/v) poly-L-lysine solution (MW 150,000 – 300,000 Da, Sigma-Aldrich, Munich, Germany) before cell seeding.
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bioRxiv - Biophysics 2021Quote: Transfected and infected cells were fixed at the indicated time points with 4 % (w/v) paraformaldeyde (Sigma Aldrich, Taufkirchen, Germany) in DPBS+/+ ...
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bioRxiv - Neuroscience 2021Quote: ... Injection solutions were prepared with a final concentration of 300ng/µl nCas9n mRNA and 10ng/µl sgrNA in nuclease free water and 0.05% (w/v) phenol red (Sigma Aldrich). Wildtype embryos were injected at the single or two cell stage with 1.5nL injection solution ...
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bioRxiv - Neuroscience 2020Quote: Tunica muscularis strips were fixed with 4 % (w/v) phosphate buffered p-formaldehyde (PFA; Sigma-Aldrich, St. Louis, MO, USA) for 20 min and rinsed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma– Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
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bioRxiv - Neuroscience 2021Quote: For neuron culture, glass coverslips (25 mm, # 1 Menzel Gläser) were coated in 0.0008 % (w/v) Poly-L-Lysine (Sigma-Aldrich, P4707) in phosphate-buffered-saline (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed again as described above and blocked in buffer (2% (w/v) bovine serum albumin (BSA; Sigma-Aldrich)/PBS ...
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bioRxiv - Neuroscience 2020Quote: 4% hydrogel monomer solution (HMS) was prepared for perfusion by mixing 40% (w/v) acrylamide (4% final concentration; V900845, Sigma), 2% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: Dried and frozen metabolite extracts were derivatized with 50 µl of 2% (w/v) hydroxylamine hydrochloride in pyridine (Sigma-Aldrich) for 60 min at 90°C ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transfection was carried out in a total of 15,000 ELT3-V cells transfected with SCR and Etv2 siRNA for 24 hours using X-tremeGENE HP DNA transfection reagent (Sigma). Cells were transfected with 10 ng of pRL_CMV vector (internal control ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and diroximel fumarate (MW= 255.23) (synthesized by T. Avery) were suspended in methylcellulose (viscosity 15 cP, 2% w/v in water; Sigma-Aldrich). For SNI anti-nociception ...
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bioRxiv - Biochemistry 2021Quote: ... the DONs were purified of excess staple strands using polyethylene glycol (PEG) precipitation (59) in the presence of 7.5% (w/v) PEG 8000 (Sigma-Aldrich). After purification ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM NaCl and 10% (w/v) glucose with an oxygen-scavenging GLOX solution (0.5 mg/ml glucose oxidase (Sigma-Aldrich), 40 µg/ml catalase (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... was positioned on a glass slide and 30 μL of KNOP medium with 1% (w/v) Gelzan CM (#G1910, SIGMA) was placed within the gene frame ...
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bioRxiv - Plant Biology 2021Quote: ... Sterilized seeds were sown on ½ MS medium [0.22% (w/v) Murashige and Skoog Basal Medium (Sigma-Aldrich M5519, Taufkirchen, Germany); 0.8% (w/v ...
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bioRxiv - Microbiology 2021Quote: Erythrocytes infected with Pf3D7 parasites at trophozoite stage were lysed in 0.05% (w/v) saponin in PBS containing a complete protease inhibitor cocktail (Sigma-Aldrich). Sequential solubilization was performed as previously described (43) ...
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bioRxiv - Cell Biology 2020Quote: ... was added at equal volume and supplemented with 10 μL anti-fade solution (9 parts glycerol, 1 part 10X PBS, 0.1 part 20%(w/v) n-propyl gallate (Sigma P3130) in dimethyl sulfoxide) ...
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bioRxiv - Plant Biology 2021Quote: ... After blocking with Tris Buffered Saline with Tween 20 (TBST) containing 5% (w/v) bovine serum albumin (BSA) (Sigma, A3608) for 1 h ...
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bioRxiv - Neuroscience 2019Quote: Mice were anaesthetized using Avertin (2,2,2-tribromoethanol 50 % w/v in tertiary amyl alcohol, diluted 1:40 in H2O; 20 ml/kg, i.p.; Sigma, Ireland) and placed in a stereotaxic frame (David Kopf Instruments ...
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bioRxiv - Microbiology 2021Quote: ... Both pre-cultures and main cultures were supplemented with 0.025% (w/v) natural L-lysine (“light” lysine, Lys0, K0, Sigma Aldrich). The expression of hipA was induced with 0.2% (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... inflorescences were soaked in 3% (w/v) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (Sigma Chemical, St. Louis, MO, USA, E6383) with 0.05% (v/v ...
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bioRxiv - Plant Biology 2019Quote: Proteins were separated on 12% or 15% SDS-PAGE gels run at 80-120 V for 2 hr and electroblotted on PVDF membrane (Millipore) at 85 V for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the cells attached to the coverslips were washed with PBS and fixed with a 4% m/v paraformaldehyde solution (PFA, Sigma) for 45 minutes at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... and immersed in modified Karnovsky’s fixative (2% w/v glutaraldehyde and 3% w/v PFA in 0.1 M sodium cacodylate buffer containing 5mM CaCl2 and 3% sucrose, all from Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Biophysics 2020Quote: ... Sections were mounted onto number 1.5 glass coverslips (Menzel-Glaser; Germany) coated with 0.05% (w/v) poly-L-lysine (Sigma-Aldrich, MO).
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bioRxiv - Cell Biology 2020Quote: ... cells were replica-plated onto pABA− (2% glucose, w/v) plates with 0 µM or 25 µM linolenic acid (C18:3, Sigma). Colonies that grew on 25 µM linolenic acid were picked into YEPD overnight cultures and struck on YEPD plates ...
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bioRxiv - Cancer Biology 2021Quote: ... The chambers were gently washed twice with PBS and filled with 100μL of blocking solution - 2.5% (w/v) bovine serum albumin (Sigma-Aldrich, A9418) in PBS - and incubated for 1 hour at room temperature ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by size by SDS-Polyacrylamide Gel Electrophoresis (SDS–PAGE) on 10% w/v gels and electroblotted onto a Polyvinylidene fluoride or polyvinylidene difluoride (PVDF) membrane (3010040001, Sigma). Luminata Crescendo Western HRP substrate (10776189 ...
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bioRxiv - Biophysics 2022Quote: ... Gels were fixed with 10% ethanol in 1× Tris-borate buffer for 30 min prior to staining with Alcian Blue solution (0.4% w/v Alcian Blue 8GX (Sigma Aldrich), 40% v/v methanol and 8% v/v acetic acid in uH2O ...
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bioRxiv - Immunology 2022Quote: ... The undigested tissue left on the gauze was scraped off with a scalpel and digested in 2.5ml 1mg/ml collagenase V (Sigma-Aldrich), supplemented with 50μl of 10mg/ml DNAse I (Roche ...
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bioRxiv - Bioengineering 2022Quote: ... and chondroitin-6-sulfate glycosaminoglycan (0.84 w/v%, CS6, Chondroitin sulfate sodium salt from shark cartilage, CAS Number 9082-07-9, Sigma-Aldrich) were homogenized in mineral buffer solution (0.1456 M phosphoric acid/0.037 M calcium hydroxide) ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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bioRxiv - Biophysics 2022Quote: ... at a dilution of 1:2000 in TBS + 1 %w/v protease-free BSA and TMB ready-to-use substrate (T0565, Sigma) was used for ELISA procedure ...
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bioRxiv - Developmental Biology 2022Quote: ... a small population (n≈10) of 3rd instar larvae were embedded in a previously heated 5% m/v carboxymethylcellulose (Sigma) solution ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of each complex was mixed with 7 μl Orange G dye (65% w/v sucrose, 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.3% Orange G powder from Sigma Life Sciences), loaded onto a 1.0% agarose gel ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were incubated in picrosirius red (PSR) staining solution 1% (w/v) Direct Red 80 (2610-10-8; Sigma-Aldrich) in saturated picric acid (P6744-1GA ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Cell Biology 2021Quote: ... were freshly oxidized by oxygen plasma and then incubated in a solution composed of 0.3% w/v 3- (trimethoxysilyl)propyl methacrylate (Sigma Aldrich) and toluene (Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... separated in a 5-20% (w/v) gradient SDS/PAGE gel (SuperSep Ace; FUJIFILM) and transferred to a PVDF membrane (Millipore). Blots were blocked with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... then blocked for 1 h with 5% w/v milk in TBS and incubated for 1 h with primary antibodies a-flag 1:4000 (Sigma) or a-myc 1:4000 (Cell Signalling) ...
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bioRxiv - Genetics 2021Quote: ... the resin was washed 5 times with ice cold DOC buffer and the bound protein eluted by 15 minute 70°C incubation in 5% w/v sodium dodecyl sulphate (SDS, Sigma). The eluted protein was reduced with 10 mM TCEP and alkylated using 20 mM Iodoacetamide ...
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bioRxiv - Genetics 2021Quote: ... The peptide bound resin was then washed 3 times with DOC buffer (1% w/v sodium deoxycholate; 50mM Tris pH9; 1X MS-SAFE Protease and Phosphatase Inhibitor, all Sigma) and stored on ice until required ...
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bioRxiv - Cell Biology 2021Quote: ... then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v) microcrystalline cellulose and 2% FCS (mixed 1:1) overlay (Sigma-Aldrich). The assay was incubated at 37°C and 5% CO2 for 70 hours then fixed in 4% PFA for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... gels and transferred (30 V; Thermo-Fisher mini-blot wet transfer system; 1 hr) to Immobilon FL polyvinylidene fluoride (PVDF) membranes (Millipore) for immunoblotting ...