Labshake search
Citations for Millipore Sigma :
6601 - 6650 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Adult ovaries were dissected after fixation and directly stored at 4°C in PBS + 0.5% (w/v) sodium azide (S2002, Sigma-Aldrich).
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: The nitrocellulose membrane was imaged for total protein and then blocked for one hour in blocking buffer (5% w/v bovine serum albumin [Sigma, SKU A7906] in TBS-T buffer [50mM Tris-HCl ...
-
bioRxiv - Physiology 2022Quote: Pancreata were injected through the portal vein with 3-5 mL cold Hanks buffer containing 0.95 mg/mL collagenase V (Sigma, USA). The collected pancreata were incubated at 37°C for 7 min ...
-
bioRxiv - Neuroscience 2022Quote: Sections were rinsed in PBS and covered with Mowiol mounting medium [9.6% (w/v) Mowiol 4-88 (Cat# 81381, Sigma-Aldrich) and 24% (v/v ...
-
bioRxiv - Synthetic Biology 2022Quote: ... An aliquot of 2 μL of the cell culture was spotted on 2xPY-agar plates supplemented with 300 μg/mL spectinomycin and 0.2% (w/v) potato starch (Sigma Aldrich). After overnight incubation at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... overnight cultures were used to respectively inoculate BHI supplemented with up to 0-0.01% (m/v) Sodium Dodecyl Sulphide or up to 20 µg/mL of tunicamycin from Streptomyces lysosuperficus (Millipore Corp) solubilized in Dimethyl Sulfoxide ...
-
bioRxiv - Microbiology 2022Quote: ... Yeast were grown on 2% (w/v) agar plates containing yeast nitrogen base (YNB) without amino acids (6.8 g/l, Sigma-Aldrich) supplemented with yeast synthetic drop out (YSDO ...
-
bioRxiv - Molecular Biology 2022Quote: ... 30 µl GFP-Trap agarose beads (proteintech) were blocked with 2% (w/v) BSA and 0.1 mg/ml yeast tRNA (Sigma-Aldrich) in lysis buffer for 1 h at 4°C to minimize non-specific interactions ...
-
bioRxiv - Plant Biology 2022Quote: ... PECTOPLATE was prepared with 0.1% (w/v) of apple pectin (molecular weight range 30,000–100,000 Da; 70–75% esterification; 76282, Sigma-Aldrich, St. Louis), 1% (w/v ...
-
bioRxiv - Neuroscience 2022Quote: ... All larvae were then anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester at pH = 7.0 (Tricaine; Sigma-Aldrich, A5040), immediately de-yolked in de-yolking buffer (55 mM NaCl ...
-
bioRxiv - Neuroscience 2023Quote: ... the diluted sample was mixed with 0.2% (w/v) CPC and with 133 mM MgCl2 (Sigma-Aldrich, cat. no. M2670) in ratio 1:1 ...
-
bioRxiv - Neuroscience 2022Quote: NKCC1-HA-Flag-mVenus membrane expression and clustering was assessed with staining performed after a short fixation at room temperature (RT) in paraformaldehyde (PFA; 4% w/v; Sigma) and sucrose (20% w/v ...
-
bioRxiv - Immunology 2022Quote: Mice were immunized intramuscularly in the quadriceps muscles of each hind leg with 50 μg OVA (OVA; grade V, Sigma) with or without 1 μL CVC1302 in 100 μL PBS or emulsified 1:2 in Marcol 52 mineral oil.
-
bioRxiv - Microbiology 2022Quote: ... was grown in 500 mL of ZYP medium [47] (supplemented with 100 μg/mL ampicillin, 34 μg/mL chloramphenicol) and 0.2% (w/v) arabinose (Sigma-Aldrich) was added to induce the expression of the chaperones GroEL/ES [44] ...
-
bioRxiv - Cancer Biology 2024Quote: ... they were perfused transcardially with a solution containing 4% (w/v) paraformaldehyde (PFA) in 1x phosphate- buffered saline (PBS, Sigma). Upon removal ...
-
bioRxiv - Neuroscience 2022Quote: ... mice were intravenously (via the tail vein) administered 2% w/v FITC-dextran in saline (70,000 Da; Sigma-Aldrich, USA) 10 minutes prior to imaging ...
-
bioRxiv - Plant Biology 2022Quote: ... Inoculated plantlet was gently inserted into the FEP tube with tweezers and medium (FAH-N2 medium pH 6.5) with 1% (w/v) low melting point agarose (Sigma Aldrich) containing fiducial markers (fluorescent beats of 1 μm in diameter ...
-
bioRxiv - Plant Biology 2022Quote: ... pH of the nutrients medium was adjusted to 7 with NaOH and jelled with 0.7% (w/v) Agar (Sigma-Aldrich). In this study ...
-
bioRxiv - Microbiology 2022Quote: ... Virus growth assays in A549 cells were performed in 1:1 DMEM/F12 medium supplemented with 0.6% bovine serum albumin fraction V (Sigma-Aldrich) and 0.2 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical Corp. ...
-
bioRxiv - Biophysics 2022Quote: Trypanosomes were efficiently immobilised in a hydrogel of 10% (w/v) vinylsulfone-functionalised polyethylenglycol (PEG-VS,tripentaerythritol core, Sigma-Aldrich) and 5.6% (w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... Expanded samples were immobilized on 25 mm (No. 1.5H) coverslips covered with 0.01 % (w/v) poly-L-lysin (Sigma-Aldrich, P8920) and imaged.
-
bioRxiv - Cancer Biology 2024Quote: ... TTK (sc-36758-V) or a non-targeting control (sc-108080) in triplicate using Polybrene Transduction Reagent (5 µg/ml; Millipore). Single-cell colonies were subsequently isolated by selection with puromycin (5 µg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... The flat mount was then rinsed with HBSS and incubated in blocking buffer made with HBSS with 5% bovine serum albumin (Catalog #BP9703-100) (W/V) and 3% Triton X-100 (Sigma) (V/V ...
-
bioRxiv - Microbiology 2024Quote: ... and the bacterial pellets were resuspended and aliquoted in MRS containing 0.3% (w/v) bovine bile (Sigma, Saint Louis, USA) for the bile tolerance assay ...
-
bioRxiv - Cell Biology 2024Quote: ... blots were blocked in 5% (w/v) milk powder in PBS (PanReac AppliChem #A0965,9100)-TWEEN® 20 (Sigma-Aldrich #P1379) (PBS-T) ...
-
bioRxiv - Bioengineering 2024Quote: ... The water phase consisted of a 2.5% (w/v) of poly(vinyl alcohol) (PVA) (87-90% hydrolyzed, average molecular weight 30,000-70,000, Sigma-Aldrich, Milwaukee, WI) in deionized water ...
-
bioRxiv - Biochemistry 2024Quote: ... used to grow transformants contained 1.92 g.L-1 (w/v) Yeast Synthetic Drop-out Medium Supplements without Uracil (Sigma-Aldrich, Y1501), 6.7 g.L-1 Yeast nitrogen base without amino acid (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... PFA fixed samples on coverslips were successively incubated in 50 mM NH4Cl in PBS (10 min) to quench the free aldehyde groups and in BSA (2% w/v, Sigma)-containing PBS to prevent non-specific binding of the subsequently used antibodies ...
-
bioRxiv - Biophysics 2023Quote: ... samples were blocked with a blocking buffer (BB) containing 5% (w/v) bovine serum albumin (BSA) (Sigma-Aldrich, Taufkirchen, Germany) in 1x PBS for 1 h at room temperature with gentle shaking ...
-
bioRxiv - Cell Biology 2024Quote: ... The excess of fixative was removed by washing with 1X PBS for 10 minutes and permeabilization and blocking were performed with a solution of 1% (m/v) BSA (Sigma) and 0.05% (m/v ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...
-
bioRxiv - Molecular Biology 2024Quote: ... incubated as necessary with primary antibody diluted in PBS-T containing 1% or 2.5% bovine serum albumin (immunoglobulin- and lipid-free fraction V, Sigma-Aldrich) and washed 3 times with PBS-T ...
-
bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
-
bioRxiv - Neuroscience 2024Quote: Mice were terminally anesthetised with an intraperitoneal injection of sodium pentobarbitone (150 mg/kg body weight) and were transcardially perfused with 4% (w/v) paraformaldehyde (PFA; Sigma) in phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2024Quote: ... cells were stained with 250 µL of a 0.1 % (w/v) aqueous CV solution (Sigma-Aldrich. St. Louis, MO, USA) for 30 min ...
-
bioRxiv - Genetics 2024Quote: ... The flower buds were then washed 3 x 2 minutes in citrate buffer and the buffer replaced with 500 μl of digestion enzyme mix (0.3% w/v cellulase from Trichoderma sp. (Sigma-Aldrich), 0.3% w/v pectolyase from Aspergillus japonicus (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... After transfer the blots were blocked in a 5 %(w/v) solution of milk powder in TBS-T (50 mM Tris, 150 mM NaCl, 0.05% Tween 20 (Sigma, P9416)) for 1h at RT ...
-
bioRxiv - Microbiology 2024Quote: ... the coupling of the PEG-acetal linker with HPV16 or fcHPV16 PsV particles was performed by immersing the PEG-coated cantilevers (∼1-to-2) in (w/v) 1% citric acid (Sigma) in milli-Q water for 10 mins ...
-
bioRxiv - Cancer Biology 2024Quote: ... dissected tumors were finely chopped with scissors and incubated with digestion buffer containing 1 mg/ml collagenase V (Sigma #C9263), 2 U/ml dispase (Life Technologies #17105041 ...
-
bioRxiv - Physiology 2023Quote: ... Colonic tissue was digested at 37°C for approximately 25 min with 0.85 mg/ml type V collagenase (Sigma-Aldrich), 1.25 mg/ml collagenase D (Sigma- Aldrich) ...
-
bioRxiv - Genomics 2022Quote: ... The undigested gelatinous tissue remnant was retrieved from the gauze and further digested with 10–15 ml collagenase V at 1.0 mg/ml (Sigma C9263) in Ham’s F12 medium/10% FBS with gentle shaking at 37 °C for 10 min ...
-
bioRxiv - Biochemistry 2022Quote: ... the membrane was washed twice with TBST and stained with Ponceau S stain [0.5% (w/v) Ponceau S (Sigma-Aldrich) in 1% (v/v ...
-
bioRxiv - Bioengineering 2023Quote: ... the excised array constructs were blocked whole overnight at room temperature in Ce3D alternative block buffer containing 1% (w/v) bovine serum albumin (BSA, Sigma), 1% (v/v ...
-
bioRxiv - Plant Biology 2023Quote: ... The sections were blocked with 5% (w/v) bovine serum albumin (BSA) in PBS and incubated then with an anti-HA mouse monoclonal antibody (Sigma) for 2 h at room temperature ...
-
bioRxiv - Plant Biology 2023Quote: ... fresh sporangia produced after crossing T0 Mprop mutants to wild-type (Tak-1 or Tak-2) were sterilised in 1 % (w/v) sodium dichloroisocyanurate (Sigma) solution for 3 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... The gelatin is made by first preparing a 15% (w/v) gelatin solution (Type A, 300 bloom form porcine skin, Sigma) by adding prewarmed PBS without Ca2+ and Mg2+ to the gelatin powder ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently incubated with 5 μL of annexin V-FITC and 10 μL of propidium iodide (Sigma-Aldrich, USA) staining solution for 15 min at room temperature in the dark ...
-
bioRxiv - Biophysics 2023Quote: ... 0.16–0.19 mm) were coated with 0.01% (w/v) poly-L-lysine (molecular weight [MW] 150,000–300,000 Da, Sigma-Aldrich, Munich, Germany) for four hours at 37°C and rinsed three times with Dulbecco’s phosphate-buffered saline containing Mg2+/Ca2+ (DPBS+/+ ...
-
bioRxiv - Cell Biology 2023Quote: ... adjusted to pH 7.2 supplemented with 0.2% (w/v) of carbon source (glucose or glycerol) (Sigma-Aldrich, St. Louis, MO) or LB (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then lysed in RIPA-buffer (50 mM Tris-HCl pH 8.0, 1% w/v NP-40 (Sigma, 492018), 0.5% w/v sodium deoxycholate (Sigma ...