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Citations for Millipore Sigma :
6901 - 6950 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: The freeze-substitution media consisted of 0.2% (w/v) uranyl acetate (SERVA Electrophoresis GmbH, Heidelberg, Germany) in absolute acetone (diluted from 20% uranyl acetate stock in methanol (Sigma-Aldrich)) ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were separated by 12% (w/v) SDS-PAGE containing 6M urea and transferred onto a PVDF membrane (Immobilion-P; Millipore, Germany) and immunodetected by protein specific antibodies.
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bioRxiv - Microbiology 2019Quote: ... The obtained mycelia were harvested and transferred to MA medium supplemented with 1% (w/v) sugarcane bagasse or cellulose (Avicel, Sigma-Aldrich) in orbital shaker at 200 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... in a semi-dry blotting chamber for 45 min at 20 V and 4 °C and crosslinked to the membrane with EDC crosslinking solution (127 mM 1-methylimidazole (Sigma-Aldrich), 163 mM N-(3-dimethylaminopropyl)-N&-ethylcarbodiimide hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... Neutrophil viability was measured using Muse Annexin V and Dead Cell Assay Kit on a Muse Cell Analyzer mini-flow cytometer (EMD Millipore).
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bioRxiv - Developmental Biology 2020Quote: ... the samples were washed an additional 5 times one hour in PBS-T and incubated in Refractive Index Matching Solution (80% w/v Histodenz (catalog no. D2158, Sigma-Aldrich) prepared in 0.02M phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: WT and LRRK2 G2019S astrocytes were cultured in 60 mm dishes to 80% confluence and immersion fixed in freshly prepared 2% (w/v) paraformaldehyde (Sigma-Aldrich) and 2% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The resultant pellets were resuspended in 100 μl of 1X PBS then incubated with 7 μl of 4% (w/v) phosphotungstic acid (Sigma-Aldrich) in 0.2 M magnesium chloride ...
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bioRxiv - Pathology 2020Quote: ... were coated with 50 μL of 0.05% w/v poly – L– lysine in carbonate – bicarbonate buffer (0.05 M carbonate-bicarbonate pH 7.4, Sigma-Aldrich, St. Louis, UK) and incubated for 60 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2020Quote: ... at 2000 rpm for 5 minutes in the aqueous continuous phase (100 mL) containing 1% w/v of poly(vinyl acetate-co-alcohol) (PVA; MW 13-23 kDa, 98% hydrolyzed; Sigma-Aldrich) as stabilizer ...
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bioRxiv - Microbiology 2021Quote: ... The remaining mosquitoes were maintained under optimal conditions in the insectary and their midgut dissected on day 7 PBM to determine prevalence and load of infection by counting oocysts on mercurochrome (0.4% w/v- Sigma-Aldrich-M7011) stained midguts using a light microscope (X20 & X40).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked and incubated with the primary and secondary HRP-conjugated antibodies in a solution with 5% (w/v) bovine serum albumin (BSA) (Sigma-Aldrich) in Tris-buffered saline solution with 0.01% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Nonspecific binding sites were blocked by incubation in 20% normal goat serum (NGS) and 5% bovine serum albumin/PBS (BSA, Fraction V; both Sigma-Aldrich). Cryosections were stained with anti TNF-α ...
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bioRxiv - Immunology 2020Quote: Nexterion-E microscope slides (Schott, Jena, Germany) were amino-functionalized with a 2% w/v linear copolymer of N,N′-dimethylacrylamide (Sigma-Aldrich) and aminoethyl methacrylate (Sigma-Aldrich ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: Cells (passage 3) were suspended in solutions of GelMA (5%, containing 1% (w/v) of the photoinitiator (PI, Irgacure 2959, Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2021Quote: ... hearts were transferred in 50% glycerol for 1 h and then mounted in anti-fade mounting media solution containing 100% glycerol + 20%(w/v) n-propyl gallate (Sigma P3130).
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bioRxiv - Genetics 2020Quote: ... scales were mounted on a microscope slide containing 10 % (w/v) Mowiol® 4-88 (Sigma-Aldrich cat# 9002-89-5) in 25% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... viable cardiomyocytes were plated at a density of 4 × 106 cells/dish on 60 mm Primaria dishes pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). After 18 h ...
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bioRxiv - Biochemistry 2021Quote: ... cardiomyocytes were plated at 1 × 106 cells/dish on 35 mm Primaria dishes containing glass coverslips pre-coated with sterile 1% (w/v) gelatin followed by laminin (20 µg/ml in PBS; Sigma-Aldrich). After 18 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The specimens were washed in phosphate buffer three times before being post-fixed in 1% (w/v) osmium tetroxide (Sigma-Aldrich) at 25°C for an hour ...
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bioRxiv - Biochemistry 2021Quote: ... after harvest the cells were rapidly mixed with an equal volume of double strength fixative [4% (w/v) paraformaldehyde (PFA, Sigma-Aldrich), 0.4% (v/v ...
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bioRxiv - Biochemistry 2021Quote: Around 30 seeds were hydrated in water for 30 min and stained with 300 µl of 0.01% (w/v) RR (Sigma-Aldrich; R2751) for 15 min at 125 rpm in 24-well plates ...
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bioRxiv - Biochemistry 2021Quote: Cellulose around hydrated seeds was stained with 0.01% (w/v) Pontamine fast scarlet 4B (S4B, also known as Direct Red 23 [Sigma-Aldrich; 212490]) in 50 mM NaCl for 60 min at 125 rpm in 24-well plates (Anderson et al. ...
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bioRxiv - Biochemistry 2021Quote: ... washed and incubated for 1-2 h at room temperature or 16 h at 4 °C with primary antibodies diluted in PBS-T containing 1% bovine serum albumin (immunoglobulin- and lipid-free fraction V BSA, Sigma-Aldrich). After extensive washes ...
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bioRxiv - Plant Biology 2021Quote: ... the leaves were stored in Aniline blue staining solution (150 mM K2HPO4, 0.01% w/v Aniline blue; Sigma-Aldrich, Munich, Germany) in the dark overnight to stain callose ...
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bioRxiv - Immunology 2020Quote: ... Typhimurium wt strain were cultured at 28°C and 37°C respectively and the bacterial suspensions were prepared with phosphate buffered saline (PBS) with 2% (w/v) polyvinylpyrrolidone-40 (PVP40, Sigma-Aldrich), as previously described (Benard et al. ...
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bioRxiv - Immunology 2020Quote: ... was incubated for 12 h at 37 °C with gentle shake with 5 μl of blood plasma in 25 μl of PBS in the presence or absence of 1% (w/v) of Protease Inhibitor Cocktail from Sigma (PIC). As control ...
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bioRxiv - Immunology 2021Quote: ... aeruginosa strains with the arabinose-inducible vector and its derivatives were grown in LB with 133 mM L-arabinose (2% w/v) and 300 μg/ml carbenicillin (both from Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... iCD4TGFBR2 and WT C57Bl/6J mice were sensitized by i.p.-injections of 20 μg ovalbumin (OVA; grade V; Sigma-Aldrich, USA) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/mL-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich,) to the cell surface ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were processed and injected into mice ovary orthotopically (n=8) followed by Doxycycline (2mg/ml) and 10% (w/v) sucrose (Sigma-Aldrich) in drinking water after 1 weeks of cells inoculation ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of 2.0 mg/mL stock solution bovine serum albumin (BSA) from fraction V powder (Sigma B-4287 or equivalent), 1,000 nM primers (forward and reverse) ...
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bioRxiv - Neuroscience 2022Quote: ... 12 μl of each sample was mixed with 120 μl of the TBA-TCA reagent (w/v 0.04% thiobarbituric acid (Kemika, Croatia) in 15% trichloroacetic acid (Sigma-Aldrich, USA). The samples were diluted with 70 μl of ddH2O ...
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bioRxiv - Bioengineering 2022Quote: ... The mouse was then fixed by transcardial perfusion with a 50 mL fixation solution containing 1×PBS and 4% w/v paraformaldehyde (PFA) (Sigma Aldrich). Mouse brain was harvested ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were washed once with 1X phosphate buffered saline (PBS) and were fixed with 4% (W/V) paraformaldehyde (PFA, Sigma, USA) solution in PBS at room temperature for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... NaCl and indium titanium oxide (ITO) coated glass slides (surface resistivity 8–12 V sq-1) were purchased from Sigma-Aldrich Co ...
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bioRxiv - Immunology 2022Quote: ... Sections were then cleared using 10% w/v CHAPS and 25% v/v N-Methyldiethanoamine in PBS for 48hrs before washing with PBS followed by 72hrs of blocking 5% donkey serum (Sigma, #D9663) in PBS ...
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bioRxiv - Genomics 2022Quote: ... ChIPs were performed at 4 °C overnight with 10 μg of antibody in a final volume of 250-μl block solution (0.5% BSA (w/v) in PBS): mouse anti-H3K27ac monoclonal (Millipore # 05-1334), rabbit anti-H3K4me2 polyclonal (Millipore # 07-030) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with phosphate buffered saline (PBS) and fixed in 4% (w/v) paraformaldehyde (Sigma-Aldrich, Mumbai, India). The fixed cells were air dried on slides and observed with 60% glycerol mountant ...
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bioRxiv - Microbiology 2022Quote: ... A yeast colony was picked from a YSM-Trp plate and resuspended in 100 µl of 5% w/v Chelex-100 (Sigma-Aldrich). Half the sample volume of acid washed glass beads were added and the sample vortexed at maximum speed for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to a pre-chilled tube then supplemented with 4uL of 15% w/v ammonium bicarbonate (Sigma-Aldrich) buffer for stable cryo-storage ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was homogenized at 20,000 RMP (Polytron) in 10 volumes (w/v) of 1X TBS (Tris buffered saline) buffer (containing protease and phosphatase inhibitor cocktail (Sigma, USA) and PR-619 (Life Sensors ...
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bioRxiv - Neuroscience 2023Quote: ... pre-weighed 50-mL conical tube containing normal drinking water or a 5% (w/v) solution of sucrose (Sigma-Aldrich 84097) was inserted in the wire lid ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mg of freeze-dried pulverized leaves in liquid nitrogen were mixed with 1 mL of chilled solution containing 10% (w/v) trichloroacetic acid (TCA; Sigma-Aldrich) in acetone (Merck ...
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bioRxiv - Plant Biology 2023Quote: ... Transfection of protoplasts with the plasmid DNA construct(s) of interest was performed by adding 1 volume PEG-Ca2+ solution (40% [w/v] PEG 4000 (Sigma Aldrich), 0.2 M D-mannitol ...
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bioRxiv - Neuroscience 2023Quote: ... Males were placed in individual small cages (26 × 20 × 135 cm) for 24 hours and were able to drink tap water or a 0.1% (w/v) saccharin solution (Sigma Aldrich, Germany) from measuring cylinders ...
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bioRxiv - Neuroscience 2022Quote: Protein samples of RIPA-soluble or RIPA-insoluble/urea-soluble fractions were separated by electrophoresis (120 V for 90 min) on a polyacrylamide gel containing 12% acrylamide in the presence of reducing agent (2-mercaptoethanol, Sigma #63689). After SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... AT depots were cut into ≈ 20 mg explants and incubated for 2 hours at 37°C in 96-well plates containing 200 μL low glucose DMEM with 5% (w/v) fatty acid-free BSA and 5 μM of Triacsin C (Sigma, T4540) per well ...
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Tryptophan specialized metabolism and ER body-resident myrosinases modulate root microbiota assemblybioRxiv - Plant Biology 2022Quote: ... ethanol and bleach with 12% (w/v) active chlorine and germinated on metal meshes placed on half-strength Murashige and Skoog (MS) basal salts (Sigma-Aldrich), supplemented with 1% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... we provided a buprenorphine solution (0.1 g/L, Temgesic®) mixed in drinking water containing 5% (w/v) D-Glucose (G8769-100ml, Sigma Aldrich) to mask the drug taste ...