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Citations for Millipore Sigma :
6551 - 6600 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the samples were mounted in polyvinyl alcohol-based mounting medium containing 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; FUJIFILM-Wako) and 2.5% (w/v) 1,4-diazabicyclo[2.2.2]octane (Sigma, St. Louis, MO, USA) as an antifade ...
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... the right hind paw of mice was inflamed by injection of 20 µl 3% (w/v) λ-carrageenan (Sigma-Aldrich), inflammation was induced while mice were under inhalation anesthesia (2% isoflurane) ...
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bioRxiv - Microbiology 2022Quote: ... and bacteria were induced for 1.5 h in MLB media supplemented with 0.5% w/v bile salts prior to infection (Sigma Aldrich B3883). Infections were synchronized by centrifugation at 1000xg for 5 minutes after addition of bacteria to wells ...
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bioRxiv - Cell Biology 2022Quote: ... The buffer was exchanged to 10 mM sodium phosphate pH 7.4 (0.036 % (w/v) sodium phosphate monobasic (Sigma Aldrich, S3139), 0.2 % (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... The scattered light intensity of the column eluant was recorded at 18 different angles using a DAWN-HELEOS MALS detector (Wyatt Technology Corp.) operating at 658 nm after calibration with the monomer fraction of Type V BSA (Sigma). Protein concentration of the eluant was determined using an in-line Optilab T-rex interferometric refractometer (Wyatt Technology Corp.) ...
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bioRxiv - Bioengineering 2022Quote: ... the PEGDA-AA hydrogels were functionalized with collagen type I (10% w/v or 0.4 mg mL-1, Sigma-Aldrich) via N – (3-Dimethylaminopropyl)-N ′ - ethylcarbodiimide (EDC)/N-Hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... was mixed into the prepolymer solution and before adding 0.005% w/v acrylic acid N-hydroxysuccinimide (previously dissolved in DMSO, Sigma-Aldrich), pH of the solution was neutralized with HCl (0.0054 % 1 M ...
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bioRxiv - Microbiology 2019Quote: ... aliquots of 100 μl of the supernatants were mixed with an equal volume of 4 % w/v Ehrlich’s reagent 4-(dimethylamino) benzaldehyde (156477, Sigma-Aldrich) in glacial acetic acid (Fisher Chemical A/0360/PB15) ...
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bioRxiv - Developmental Biology 2019Quote: ... WT and Etv5 KO mESCs (~2×105 cells) were collected and resuspended with 1×Binding Buffer containing Annexin V-FITC/PI (Sigma). The cells were incubated at room temperature in the dark for 5 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... and digested at 37°C in Hank’s balanced salt solution containing 2 mg/ml type V collagenase (Sigma-Aldrich, C9263) for 30 min under constant agitation ...
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bioRxiv - Developmental Biology 2019Quote: ... animals were anesthetized by bath application of 0.02% w/v solution of Ethyl-3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis) in filtered fish system water for 1 min ...
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bioRxiv - Developmental Biology 2019Quote: ... The flies then transferred to the regular food supplemented with 0.5% (w/v) Acid Blue 9 (erioglaucine disodium salt, Sigma 861146) for 4 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse brains were perfusion-fixed in 4% paraformaldehyde and embedded in low-melting point oxidized agarose (4.5% w/v; Sigma #A0169). Vibratome sections were prepared at 75 µm thickness using a frequency of 70 Hz and a speed of 0.5 mm/sec ...
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bioRxiv - Molecular Biology 2019Quote: ... NP-40 and 0.02% (w/v) sodium azide in distilled H2O with the addition of 1X EDTA-free SIGMAFAST protease cocktail tablet (Sigma). To quantify protein in the tissue lysates ...
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bioRxiv - Plant Biology 2020Quote: ... two-week-old plantlets from liquid culture were vacuum-infiltrated with 0.1% (w/v) nitroblue tetrazolium (NBT; Sigma-Aldrich, Germany). Relative saturation was quantified on images of single leaves and compared between groups by nonparametric testing (Supplemental Methods ...
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bioRxiv - Pathology 2021Quote: ... Stage V-VI oocytes were selected after enzymatic defolliculation (2 h of gentle shaking in a collagenase-containing solution (1A, Sigma) at 16°C) ...
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bioRxiv - Plant Biology 2021Quote: ... Fixed plant material was digested for 0.5 to 1.5 hours at 37° C in an enzyme mixture consisting of 2 % (w/v) cellulase from Aspergillus niger (Sigma C1184), 4 % (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Colonies were fixed with Carnoy’s solution (1 acetic acid: 3 methanol) and stained with 1% (w/v) crystal violet (Sigma-Aldrich). The percentage of colonies covering the plates were calculated with ImageJ software.
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bioRxiv - Biophysics 2019Quote: ... a cell suspension [107 neurons/mL] in 0.15% (w/v) sodium alginate (Pronova UP LVG, Novamatrix, Norway) was loaded into donut-shaped (3mm inner diameter) 0.8% agarose (Sigma-Aldrich) molds which were surrounded by Neurobasal medium (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: ... To test the effects of mucin addition43 and iron depletion,44 overnight-grown cultures were inoculated in a 1:200 dilution in LB supplemented with different components [0.4% (w/v) of bovine submaxillary gland mucin (Sigma-Aldrich), or 200 µM of 2,2’-dipyridyl (Alfa Aesar) ...
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bioRxiv - Cell Biology 2019Quote: ... 5’-GGTCAGCCAACTCGTCACAGTC-3’) and by alizarin red S staining with a 2% (w/v) alizarin red S solution (Sigma-Aldrich). Slides were imaged in an Olympus BX61 microscope ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue pieces were digested with 0.05% w/v trypsin and 0.5mg/ml DNAse I (both from Sigma, #T4549, #DN25) in phosphate-buffered saline for 10min ...
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bioRxiv - Systems Biology 2021Quote: ... chondrocytes after three passages in monolayer were re-suspended in 1.2% (w/v) low viscosity alginate (Kelton LV alginate, Kelko Co, San Diego, CA, USA) in 0.9% NaCl (Sigma Aldrich) at a concentration of 4 × 106 cells/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Amyloid was visualized using a 2-minute incubation in Thioflavin S (0.5% w/v; #T-1892, Sigma, St. Louis, MO) followed by a brief wash in 70% ethanol as described previously5 ...
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bioRxiv - Bioengineering 2020Quote: ... One solution contained a 10% w/v 4-arm PEG-vinyl sulfone (20 kDa, JenKem USA) in 300 mM triethanolamine (Sigma), pH 8.25 ...
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bioRxiv - Cell Biology 2021Quote: ... Skin was minced into small pieces (about 2-3 mm × 2-3 mm) and digested by incubation with 1 mg/mL collagenase V (Sigma) in PBS for one hour with shaking (250 rpm) ...
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bioRxiv - Biophysics 2020Quote: ... Syn7942 cells were incubated at 30°C with 25 mM NaH14CO3 for 2 minutes before permeabilization with 0.03 % (w/v) mixed alkyltrimethylammonium bromide (Sigma-Aldrich) and RuBP (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Seeds were surface-sterilized and sown in Petri dishes on half-strength Murashige and Skoog (MS/2) solid medium containing 0.8 % (w/v) agar-agar type E (Sigma Aldrich) and 1% sucrose ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked in 5% (w/v) non-fat dried milk powder in 1x Tris-buffered saline with 0.5% Tween20 (Sigma-Aldrich) for 1 h and then incubated in the appropriate primary antibody over night at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Ring stage synchronisation (∼0−18 h p.i.) was achieved by a single treatment of infected RBCs with 5% (w/v) D-sorbitol (Sigma-Aldrich), unless otherwise indicated.
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bioRxiv - Immunology 2020Quote: ... The undigested tissue left on the gauze was scraped off with a scalpel and digested in 2.5ml 1mg/ml collagenase V (Sigma-Aldrich), supplemented with 50ul of 10mg/ml DNAse I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole, pH 8.0) to 2 mg/mL, and 0.6% n-Dodecyl β-D-maltoside (DDM, w/v) (Sigma-Aldrich) and 0.01% lysophosphatidylcholine (lyso-PC ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% Tween-20 with 5% w/v nonfat dry milk) and incubated with diluted (1:10.000) rabbit polyclonal antiserum (Sigma-Aldrich) against mCherry overnight ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM NaCl and 10% (w/v) glucose with an oxygen-scavenging GLOX solution (0.5 mg/ml glucose oxidase (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2022Quote: ... catheters were flushed daily with 0.20mL of heparinized saline (50 U/mL in 0.9% saline; Sandoz Canada Inc) containing gentamicin sulphate salts (10% w/v; Sigma-Aldrich) as a prophylactic antibiotic.
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bioRxiv - Cancer Biology 2022Quote: ... Tumor fragments were then transferred to a solution of type V collagenase (Sigma C9263, 1 mg/mL in 1X HBSS) supplemented with soy trypsin inhibitor (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... and tumor fragments were transferred to a solution of type V collagenase (Sigma C9263, 1 mg/mL in HBSS 1X) and incubated at 37 C for 45 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Fatty acids were then methylated with 2 mL of Boron Trifluoride (BF3) in Methanol (14% W/V) (Sigma Aldrich, USA) and incubated at 55 °C for 1.5 hours with intermittent shaking every 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was removed and cells were suspended in 9.8 μl Annexin V binding buffer + 0.2 μl Calcofluor White Stain (Sigma-Aldrich, Fluka). Images were acquired using Axio Observer z1 (Zeiss).
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: ... Colonic tissue was simultaneously digested under the same condition for approximately 25 min but with the mixture of 0.85 mg/ml type V collagenase (Sigma-Aldrich), 1.25 mg/ml collagenase D (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... Seeds were germinated in soil or were surface-sterilized with 30% bleach/0.02% Triton-X100 solution and germinated on half-strength MS (Murashige and Skoog, 1962) basal salt medium supplemented with 1% (w/v) sucrose and 0.3% phytagel (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: ... The 300 μL mixtures were adjusted to a 40% OptiPrep Density gradient medium by mixing with 200 μL 100% (w/v) OptiPrep Density gradient medium (Sigma). The mixture was transferred to a 3.2 mL ...
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bioRxiv - Bioengineering 2022Quote: ... the glass coverslip used to localize the beads was removed, and a 0.2% (m/v, in PBS) gelatin (Sigma G1393) coating was conjugated to the polyacrylamide gels using N-ethyl-N’-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 % (w/v) alizarin red (AR) (pH 4.1) to determine mineral deposition via calcium staining (all from Sigma-Aldrich). Stained sections were imaged using an Aperio ScanScope slide scanner and thickness measurements obtained using Aperio Imagescope.
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bioRxiv - Cancer Biology 2022Quote: ... were treated for 20 min RT with Poly-L-lysine solution 0.1 % (w/v) in H2O solution (cat# P8920 from Sigma-Aldrich). After two washes with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... we filtered cell suspensions through a 100mm cell strainer and added 3ml 0.1% w/v soybean trypsin inhibitor to stop the digest (Sigma# T9128). The suspensions were then filtered through a 40mm cell strainer ...
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bioRxiv - Cell Biology 2022Quote: ... Two mg of dry GelMA and gelatine were dissolved in 1mL of 4% w/v sodium bicarbonate solution (Sigma-Aldrich) and 1mL of 0.5% w/v TNBS solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and dissociated to a single-cell suspension in Hanks’ Balanced Salt Solution containing 2.25% (w/v) BSA (Sigma, Cat. H9269) and 1 mg/mL collagenase (Sigma ...