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Citations for Millipore Sigma :
6851 - 6900 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Each 250 mL flask contained 70 mL of a defined minimal media supplemented with 0.075% (w/v) low viscosity alginate soluble from brown algae (Sigma-Aldrich, A1112) and bacterial cells at an A600 of 1.0 were diluted 10−3 ...
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bioRxiv - Neuroscience 2019Quote: ... 25-40 animals were incubated for 3h at 20°C in 5% (w/v) of blue food dye (Erioglaucine disodium salt, Sigma-Aldrich) in E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Stage V-VI oocytes were manually separated and enzymatically defolliculated using a 1 mg/mL collagenase (Sigma-Aldrich, Milwaukee, WI, USA) solution (calcium-free ND96 buffer ...
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bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Eggs on the filter were exposed for 10 min to 0.01% (w/v) mercuric chloride (Sigma-Aldrich, St Louis, MO, USA), followed by 0.7% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... cells were transferred (2% inoculation) into 50 ml of T-R minimal medium supplemented with 0.5% (w/v) N-acetylglucosamine (Sigma-Aldrich #G4875) as a carbon source and incubated ~12 hr at room temperature and 150 rpm ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA and 0.1% (w/v) SDS] with 1 mM PMSF and protease inhibitor cocktail (Sigma-Aldrich, St. Louis, USA) followed by sonication ...
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bioRxiv - Developmental Biology 2019Quote: ... Stained embryos were washed three times with 1x Holt’s buffer and then anesthetized with 0.02% w/v MS-222 (Sigma-Aldrich, cat. #A5040) before the analysis ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were cleaned in phosphatebuffered saline (PBS) for three times and stained in PI/FITC-Annexin V with 50 μg/ml RNase A (Sigma-Aldrich). Keep cells in dark processing at the room temperature for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Isolated actinomycetes strains were spot inoculated on a modified agar screening media with 1% (w/v) Avicel® PH-101 (Sigma) as a substrate for exo-β-1,4-glucanase and 0.2% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... STORM imaging was performed in fresh imaging buffer (50mM Tris-HCl, pH 8.0, 10mM NaCl, 10% (w/v) glucose (Sigma, cat#G8270), 1.5mg MEA (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to a pre-chilled tube then supplemented with 4uL of 15% w/v ammonium bicarbonate (Sigma-Aldrich) buffer for stable cryo-storage47 ...
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bioRxiv - Plant Biology 2020Quote: ... The fixative was washed away with phosphate buffered saline buffer supplemented with Tween 20 (PBST) and cells were treated for 8 min at room temperature with a solution of 2% (w/v) Driselase (Sigma-Aldrich) in 0.4 M mannitol ...
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bioRxiv - Biochemistry 2021Quote: ... A 150-µl aliquot was mixed with an equal volume of Annexin V buffer containing 5 mg/ml fatty acid-free BSA (Sigma-Aldrich) and 5 nM SYTOX red (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... a stock solution of 2% w/v agarose was initially generated by mixing the requisite amount of agarose (A9414, Millipore-Sigma) and DI water at 70 °C until dissolved ...
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bioRxiv - Bioengineering 2020Quote: ... The PA and OA stock solutions were individually prepared by dissolving in phosphate-buffered saline (PBS) containing 30% (w/v) BSA (Sigma-Aldrich) solution at 50 mM for each sample ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Bioengineering 2020Quote: ... We initiated polymerization of each 500 μL gel precursor aliquot by adding 5 μL 10% w/v ammonium persulfate (APS; Sigma A9164) and 0.5 μL N,N,N”,N”-Tetramethylethylenediamine (TEMED ...
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bioRxiv - Neuroscience 2021Quote: ... Retinas were rapidly dissected in 1X phosphate-buffered saline (PBS) and transferred to 4% (w/v) paraformaldehyde (Sigma Aldrich, P6148-1KG) for 30 mins fixation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... H3255 and H3255-TM were maintained in ACL4 medium formulated in DMEM:F-12 including: Bovine Serum Albumin 0.5% (w/v) (Sigma cat no. A8022), apo-Transferrin (human ...
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bioRxiv - Cell Biology 2021Quote: Transferrin uptake assays were performed by serum-starving cells in DMEM/f12 with 0.5% (w/v) BSA (Sigma Life Sciences A9647) for 30 minutes followed by adding fresh DMEM/f12/ BSA with alexa-fluor 647-labeled transferrin at a concentration of 10 micrograms/milliliter (Jackson Immunoresesarch 009-600-050) ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Microbiology 2020Quote: ... 100 µL of cell-free spent culture media served as a crude enzyme source was mixed with 100 µL of 1% (w/v) chitin powder (Sigma, USA) in 50 mM sodium phosphate buffer (pH 6.5) ...
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bioRxiv - Systems Biology 2021Quote: ... with 100 µl of a 2 % (w/v) solution of Evans blue dye in 0.9 % saline (Sigma–Aldrich Ltd, Poole, UK). Dye was permitted to circulate for 1 h before animals were transcardially perfused with 0.9 % saline at 4 °C to remove circulating dye ...
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bioRxiv - Microbiology 2021Quote: ... the plates were washed three times with PBS-T (see above) and were blocked with 200 μl of 1% (w/v) BSA (Sigma Aldrich) in PBS-T for one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
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bioRxiv - Biophysics 2020Quote: The C85A/L194A and C85A/L194A/H234C mutant CLC-ec1 protein crystals were grown in 29% (w/v) PEG400 (Sigma-Aldrich), 200 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... RBCs were allowed to settle onto poly-L-lysine coated coverslips (squeaky clean coverslips were coated with a 0.1% (w/v) solution of poly-L-lysine (Sigma-Aldrich P8920) 1-2 h room temp with poly-L-Lysine then dried and kept at 4°C until ready for use) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde for 10 minutes and stained with 0.5% w/v crystal violet solution (Sigma-Aldrich, UK) for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and washed in 0.05% v Brij-35 in PBS (pH 7.4) and immunostained using antibodies to MUC5AC (Millipore Inc., Burlington, MA), SCGB1A1 or secretoglobulin 1A1 (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... Each of them contained 30 individuals and was fed with a 50 % (W/V) sucrose solution mixed with PGN (Sigma, USA) at different concentrations (0 ...
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bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were treated with 0.8 M mannitol [32] with 0.1% (w/v) Calcofluor white (Sigma-Aldrich, cat. n. 18909) for 30 min ...
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bioRxiv - Immunology 2021Quote: The right caudal lope of the lung was removed from the body and transferred on ice in PBS containing 1% BSA (w/v) and 2 mg/ml Actinomycin D (Sigma-Aldrich) for further processing ...
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bioRxiv - Plant Biology 2021Quote: ... The regenerating plantlets were cocultivated with the agrobacterium strain GV2260 harboring the binary vector in liquid 0M51C medium containing 2% (w/v) sucrose and 100 μM acetosyringone (Sigma-Aldrich) at 22 °C under continuous white light for four days ...
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bioRxiv - Neuroscience 2021Quote: ... the vasculature in PdgfrβCre-tdTomato and Col1a2CreER-tdTomato mice was visualized with 25µL of 5% (w/v in saline) 70kDa FITC-dextran (Sigma-Aldrich; 46945). During imaging ...
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bioRxiv - Neuroscience 2021Quote: Mice were deeply anesthetized and then perfused transcardially with 20 ml PBS to wash out the blood and 20 ml 4% (w/v) PFA (Sigma-Aldrich) for fixation ...
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bioRxiv - Neuroscience 2021Quote: ... Samples processed for immunohistochemistry using antibodies against NFIB were fixed for a shorter period of time (10 minutes) and were pre-treated with 1% (w/v) saponin (Sigma-Aldrich) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The assembled microfluidic device was degassed in a vacuum desiccator for ~30 minutes prior to loading a 5% w/v poloxamer surfactant (Sigma P5556) with 2% xylene cyanol (2 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... At this point parasites were centrifuged at 2000 RPM for 3 min and the pellet was re-suspended in 100 µL of PBS containing 20 µg of propidium iodide or Anexin V (Sigma-Aldrich) and incubated for 1 h in the dark at 4 °C ...
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bioRxiv - Biophysics 2021Quote: Cleaning of microscopy glasses followed by coating of Knittel coverslips with 0.5% (w/v) bovine serum albumin (BSA, Sigma-Aldrich A2153) was carried out to provide a hydrophilic surface ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples from brain and peripheral organs were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/ml-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich) to the cell surface ...
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bioRxiv - Immunology 2021Quote: Mice were sensitized to 20 g ovalbumin (OVA) (Grade V, 2.25 mg alum in saline, pH 7.4; Sigma, St. Louis, MO) by intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2022Quote: ... ML-099 (SML1258), Rac1 inhibitor V (5308200001), rhosin (555460), thapsigargin (Tg, cat#) and tunicamycin (Tm, cat#) were all purchased from Sigma-Aldrich. N9-methyl 6- thioguanine (6-TG-Me ...
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bioRxiv - Systems Biology 2022Quote: Synchronized wildtype adult worms were washed and incubated with 0.1% ThS from a 10% stock (w/v, dissolved in DMSO; ThS (SIGMA, T1892-25G) in 500 µL of liquid NGM for 3 hours in the dark ...
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bioRxiv - Microbiology 2022Quote: ... The sample was washed twice using 50 mM Tris HCl containing 25 mM NaCl at pH 8.5 and 0.5% w/v low gelling agarose solution (Sigma-Aldrich, #A9414-100G) was carefully added to the top to ensure proper immobilization ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
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bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...