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Citations for Millipore Sigma :
6451 - 6500 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed using the Duolink® In Situ Red Kit Mouse/Rabbit (Sigma-Aldrich, Cat # DUO92101). For in vitro PLA ...
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bioRxiv - Developmental Biology 2023Quote: ... Two New Zealand White rabbits were injected at ten sites with 500 µl of Freund’s Complete Adjuvant (Sigma-Aldrich F5881) containing 500 µg of Tin protein ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Microbiology 2023Quote: ... and probed with 1:1000 anti-MntS (rabbit polyclonal) followed by 1:10,000 goat anti-rabbit-AzureSpectra800 (Azure) or 1:1000 anti-FLAG M2-HRP antibody (Sigma-Aldrich) in 3% milk-TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked with 3% BSA/PBS for 1 hour and incubated with rabbit anti-Olig2 (1:500, Millipore), mouse anti-CC1 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed six times with TBST buffer and blotted with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (Sigma). After washing with TBST buffer again ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA reactions were subsequently performed using Duolink PLA probes for goat-mouse and goat-rabbit and Duolink In Situ Detection Reagents Red (Sigma) following the manufacturer’s protocol ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore; anti-D1R ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with secondary-HRP conjugated antibodies (1:10.000; Anti Rabbit IgG HRPO, Millipore BI2407; Anti Mouse IgG HRPO, Millipore BI2413C) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... We then washed the slices 5 times in PB 0.1 M and incubated them with primary antibody rabbit IgG antiGABA (Sigma A2052) diluted in PB 0.1 M 1:1000 with 1% NGS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 100uL of Pierce Protein A/G Magnetic Beads were coupled to 10 µg of antibody or control rabbit IgG antibody (Sigma). DNA was incubated with antibody-coupled beads and after uncoupling ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in PBS and incubated with rabbit anti-TH primary antibody (1:200, Millipore AB152, RRID: AB_390204) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked and incubated overnight with a primary antibody against PGP9.5 (rabbit polyclonal antibody, Sigma-Aldrich, SAB4503057; 1:100) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and stained with anti-rabbit IgG antibody conjugated to Alexa488 (Sigma- Aldrich, SAB4600045) and anti-mouse IgG antibody conjugated to Alexa647 (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Bioengineering 2023Quote: ... and incubated for 4 hours with an anti-rabbit secondary antibody coupled with Rhodamine (#SAB3700846 Sigma-Aldrich, dilution 1:200). Cells were incubated in AlexaFluor 488 phalloidin (#R37110 ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich), α-ZC3HAV1 (GTX120134 ...
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bioRxiv - Immunology 2022Quote: ... The lysate was immunoprecipitated for 4 h at 4°C with 10 μg polyclonal rabbit α-ZFP36L1 (ABN192, Sigma-Aldrich) or with isotype control (12-370 ...
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bioRxiv - Genetics 2022Quote: ... for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma). Chemiluminescence detection was performed by using ECL Western Blotting detection Reagent (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... (gift from the laboratory of Jeremy Nathans) rabbit anti-LW/MW-opsins (1:200 for organoids, 1:500 for human retinas) (AB5405 Millipore), and mouse anti-Rhodopsin (1:500 ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... After treatment with the secondary antibody (anti-rabbit HRP-conjugated, 1:1000 or anti-mouse HRP-conjugated, 1:1000, Sigma), membranes were incubated in chemoluminescent (SuperSignal West Femto ...
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bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and primary antibodies (SDC1 Rabbit Thermo Fisher Scientific, MA5-32600, 1:500 dilution; GFAP mouse Sigma G3893, 1:500 dilution). After extensive PBS washings ...
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bioRxiv - Neuroscience 2023Quote: ... then with the primary antibodies in the same solution overnight at 4°C (SDC1, Rabbit Thermofisher MA5-32600, 1:500 dilution; GFAP, mouse Sigma G3893 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Goat anti-mouse IgG-HRP or Goat anti-rabbit IgG-HRP, Sigma) diluted in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-β-actin (A5441, clone AC-15) and rabbit polyclonal anti-Calnexin (#C4731) were obtained from Sigma-Aldrich. Mouse monoclonal anti-V5 (BLD-680605 ...
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bioRxiv - Cell Biology 2023Quote: Polyclonal antibody against the C-terminus of mouse SETX-C were generated by immunizing rabbits. Mouse cDNA encoding Setx (aa. 1900-2701) was subcloned into pET15b (Novagen) and transformed to E.coli strain BL21-CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed and incubated with H3K27me3 antibody (1:200 dilution, Cell signalling 36B11#9733, control with IgG rabbit Sigma) for 2h30 at 4 °C on a rotating wheel and then washed twice ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were then washed 3 times for 20 min in PBS-Triton before incubation with anti-rabbit Alexa Fluor 546 used at 1:500 dilution in blocking buffer containing Hoechst 33342 (Sigma) to label the nuclei ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-HA antibody (Sigma) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used were: a rabbit polyclonal anti-GFP in a 1:400 dilution (Millipore Sigma, AB3080, lot: 3717722), a mouse monoclonal anti-NeuN in a 1:500 dilution that is a marker for mature neurons (Millipore Sigma ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed with PBS-0.1% Tween 20 and then incubated with 100 μl of 100 μg/well of biotinylated rabbit anti-human C1q (Sigma) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... as primary antibodies and Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Immunology 2024Quote: ... before microtubule-associated protein light chain 3 (LC3)-I and LC3-II levels in cell lysates was analysed by immunoblotting (rabbit polyclonal LC3 antibody, Sigma).
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used in this study were: polyclonal rabbit anti-human HINT1 antibody (1:1000, Sigma, San Luis, MO, USA), and to demonstrate equal loading mouse monoclonal anti-β-actin antibody (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... The precleared supernatant was divided into two Eppendorf tubes and beads were either cross-linked to the bait antibody (Grin2b-rabbit #06-600 Millipore,Ppp1ca-Abcam Rabbit #ab16446 ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were obtained using horseradish peroxidase-conjugated antibodies against mouse whole immunoglobulins and horseradish peroxidase-conjugated antibodies against rabbit whole immunoglobulins (1∶10,000, Sigma-Aldrich) as secondary antibodies and detection with the Lumi-Light Plus Western Blotting Substrate (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were subsequently carried out using Duolink PLA plus and minus probes for rabbit and mouse (respectively) and Duolink In Situ Detection Reagents Orange (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoblots of BN-PAGE analysis were decorated with HRP-coupled goat anti-mouse or HRP-coupled goat anti-rabbit (both Sigma) as secondary antibodies (dilution ...
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bioRxiv - Plant Biology 2024Quote: ... Then the membrane was incubated with another primary serum and revealed using an anti-rabbit immunoglobulin G coupled to alkaline phosphatase (Sigma). Data were acquired from three or four independent experiments and representative immunoblots are shown ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used: Rabbit anti-Orai1 (1:50, from Dr. Stefan Feske) and mouse anti-ORAI1 (1:50, SAB3500126, SIGMA), rabbit anti-STIM1 (1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate production was enzymatically quantified as NADH fluorescence (360 nm/460 nm) by the reverse reaction of L-lactate dehydrogenase (Rabbit muscle, L25005KU, Sigma) in a reaction containing 20 µL cell media ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with rabbit complement sera (1:3 dilution, Cat. no. S7764, Sigma) for 30 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore; sheep anti-TH ...