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Citations for Millipore Sigma :
6251 - 6300 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Antibody against vinculin was purchased from HRP-conjugated antibodies against mouse IgG (NA931V; 1:10 000) and rabbit IgG (NA934V; 1:10 000) were purchased from Sigma. Immunohistochemistry was performed by Molecular Pathology (Robarts Research Institute ...
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bioRxiv - Immunology 2019Quote: ... Sections (14 um) were cut and stained with primary antibodies (rabbit anti-CGRP, Millipore, PC205L, 1:500; guinea-pig anti-TRPV1, Millipore, AB5566 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used include anti-α and β-Synuclein (EP1646Y, N-terminal rabbit monoclonal, 1:100 dilution; Millipore, Billerica, MA), anti-pS129 αSyn (81A ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594-conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Microbiology 2019Quote: ... samples were then stained using Alexa 488-conjugated anti-mouse and Alexa 594-conjugaed anti-rabbit antibodies and DAPI (Sigma) for 2 hours at room temperature ...
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bioRxiv - Physiology 2019Quote: ... anti-Rabbit secondary (Cat. No# A0545) and anti-mouse secondary (Cat. No# A9044) antibodies were obtained from Sigma Aldrich (USA).
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bioRxiv - Immunology 2019Quote: ... Secondary HRP-conjugated antibodies (polyclonal goat anti-mouse IgG at 1/1000 or anti-rabbit IgG at 1/2500 from Millipore) were used to detect antibody binding ...
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bioRxiv - Neuroscience 2019Quote: ... Signals were obtained by binding of a secondary anti-rabbit horse radish peroxidase (HRP) linked antibody (1:15000, Sigma-Aldrich) and visualized by exposing the membrane to a charge-coupled device camera (LAS1000 ...
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bioRxiv - Neuroscience 2019Quote: Membranes were blotted with primary antibodies including rabbit polyclonal anti-GluN1 C- terminus (1:2000, Sigma-Aldrich Cat# G8913, RRID:AB_259978), rabbit polyclonal anti-GluN2A N-terminus (1:500 ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... the NC membranes were incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP; Sigma-Aldrich; dilution, 1:8000) and the color reaction was examined using ECL Kit (Amersham Pharmacia Biotech ...
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bioRxiv - Molecular Biology 2019Quote: ... blots were subsequently incubated for 1h at room temperature with horseradish peroxidase-conjugated secondary anti-rabbit/mouse antibodies (1:10000; Sigma). Immunoreactive bands were visualized by chemiluminescence detection of peroxidase activity (SuperSignal West Dura ...
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bioRxiv - Cell Biology 2019Quote: ... Duolink® In Situ PLA Rabbit Plus and Mouse Minus probes were used in combination with Detection Reagent Green (Sigma) following manufacture’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... The phenotype of IdU-IR cells and CldU-IR cells was determined using rabbit anti-calbindin antibodies (1/200, Millipore) that were revealed with Alexa 488-goat anti-rabbit IgG antibodies (1/500 ...
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bioRxiv - Plant Biology 2020Quote: ... Four washes of 15 min each in TBST at room temperature and then it was incubated two hours with a secondary antibody anti-rabbit (goat) conjugated with alkaline phosphatase (A3687, Sigma), in a 1:2,500 dilution with TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with primary antibodies (rabbit anti-miniSOG 1:500 [Tsien Lab]; guinea pig anti-VGLUT2 1:2000 [Millipore #AB2251]; rabbit anti-TH 1:1000 [Millipore #AB152] ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with primary antibodies (rabbit anti-miniSOG 1:500 [Tsien Lab]; guinea pig anti-VGLUT2 1:2000 [Millipore #AB2251] ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then stained and visualised for β-actin (rabbit anti-β-actin HRP, Sigma-Aldrich, A3854, 1:50,000) as described above.
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bioRxiv - Cell Biology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink® In Situ Red Mouse/Rabbit kit (DUO92008, Sigma-Aldrich, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... followed by horseradish peroxidase (HRP)- conjugated swine anti-rabbit (1:5000) while Mrc1-FLAG in input samples was detected by HRP-conjugated rabbit anti-FLAG (1:3000, A8592, Sigma). Rabbit anti-Ctf4 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... and goat serum (15 min, Maixin, Fuzhou, China) and then incubated with rabbit anti-TH antibody (1: 1,000, AB152, Millipore, USA) overnight at 4 °C ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901]; mouse anti-Pax6, 1:200 [Millipore #MAB5552] ...
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bioRxiv - Biochemistry 2020Quote: ... membranes were incubated for 1 hour at room temperature with 1:10,000 diluted peroxidase-conjugated goat anti-rabbit IgG (Sigma, A6154). After 4 washes in PBS with 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat. # G4595) and mouse monoclonal antibody against GAPDH (Cat. # G8795) were purchased from Sigma Aldrich. Rabbit polyclonal antibody against Peroxiredoxin-SO3 (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated with secondary antibodies (mouse, 1:10000 dilution, A9044, Sigma; rabbit, 1:10000 dilution, A9169, Sigma). Immunoreactive bands were visualized by ChemiDoc XRS+ imaging system ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies or lectin were used: an anti-aggrecan rabbit antibody (1:4500 for DAB staining; 1:500 for fluorescence staining; AB1031, Merck Millipore), an anti-GABA chicken antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated at 4°C for 48h in the primary antibody solution (anti-TH rabbit, Millipore, AB152(CH), 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slices were blocked with the blocking solution (10% normal goat serum and 0.7% Triton X-100 in PBS) for 2-h at room temperature and subsequently incubated with primary antibodies (rabbit anti-Glutamate Sigma, #G6642 ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies were used at the following concentrations for immunohistochemistry: rabbit anti-NF200 (1:500, Sigma, St. Louis, MO, #N4142), IB4-biotin conjugate (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... blocked in normal donkey serum in PBST (2%) and transferred to rabbit anti-Fos primary antibody (1:10000; EMD Millipore) in PBST and normal donkey serum for 16 hr at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal, 1:1000 dilution, Sigma), α-tubulin (mouse monoclonalDM1A ...
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bioRxiv - Biochemistry 2021Quote: ... or 680LT-conjugated anti-rabbit IgG (1:5000, Li-COR) and IRDye 800CW (Li-COR) labeled HPA (Helix pomatia lectin, Sigma) or PNA (peanut agglutinin ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262, 1:1,000; rabbit anti-vasopressin, Sigma-Aldrich AB1565 ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Plant Biology 2020Quote: ... followed by incubation in a 1:200 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot of phosphorylated MAPKs was performed using anti-phospho p44/42 MAPK antibody as the primary antibody and peroxidase-conjugated goat anti-rabbit IgG (Sigma) as the secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Microbiology 2021Quote: ... The deposited C5b9 complex in the reaction mixture was detected using rabbit anti-C5b-9 pAb at 1:1000 dilution (204903; Sigma) and HRP conjugated anti-rabbit pAb (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... in 5% BSA-TTBS overnight at 4°C and then HRP-conjugated goat anti-rabbit IgG (Jackson) or goat anti-mouse IgG(Sigma) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Flag (M2) and anti-MAP2 (HM-2) antibodies as well as polyclonal rabbit anti-Flag antibodies (F3165, F7425) were from Sigma. Anti-Xpress antibodies were from Invitrogen (46-0528).
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bioRxiv - Neuroscience 2022Quote: ... 0.3% Triton X-100 in PBS for 1 hour and then incubated with rabbit anti-TH (1:1000; Millipore: AB1542) and sheep anti-fibrinogen (1:300 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell line showing reduced intensity or no bands in western blot using rabbit anti-horseradish peroxidase antibody (P7899, Sigma-Aldrich) which can recognize core α1,3-fucose and β1,2-xylose were propagated and used for further analysis (supplementary figure 1 ...
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bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...
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bioRxiv - Immunology 2022Quote: ... SAMD9 knock-down was confirmed by Western Blot using rabbit anti-SAMD9 antibody (Cat# HPA021319, Millipore-Sigma, St. Louis, MO) and goat-anti-rabbit HRP secondary antibody (Cat# 111-035-144 ...
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bioRxiv - Immunology 2022Quote: ... SAMD9 knock-down was confirmed by Western Blot using rabbit anti-SAMD9 antibody (Cat# HPA021319, Millipore-Sigma, St. Louis, MO) and goat-anti-rabbit HRP secondary antibody (Cat# 111-035-144 ...