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Citations for Millipore Sigma :
6401 - 6450 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the samples were incubated with primary antibodies (anti-β-tubulin III produced in rabbit (Sigma, Ireland, T2200, 1:400) and anti-GFAP produced in mouse (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were incubated at 4°C for 16 hours with rabbit anti-Myc (1:500, C3956; Sigma-Aldrich) primary antibody in PBS/0.5% normal donkey serum/0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by probing with respective goat anti-mouse or rabbit secondary antibodies conjugated with alkaline phosphatase (dilution 1: 5,000; Sigma-Aldrich) or conjugated with HRP (dilution 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... for 120 minutes followed by overnight incubation at 4°C with a 1/200 dilution of the poly-clonal rabbit sera and antibodies for histone H3 (final concentration 2.5 μg/mL, Sigma SAB4200651) in blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-human βIII-tubulin (1:500; Covance, cat. MRB-435P) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Primary antibodies were tagged using either goat anti-mouse AF647 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for all stainings were as follows: rabbit anti-TGFA (HPA042297, Sigma-Aldrich, dilution 1:50 and 1:100), rabbit polyclonal anti-pEGFR clone Tyr845 (07-820 ...
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bioRxiv - Cell Biology 2022Quote: ... Dylight 550-labeled rabbit anti-BUB-1 (custom made, 74) and FITC-labeled mouse anti-α-tubulin (SIGMA F2168, DM1α) were used at a concentration of 1 μg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... MyoD (primary Anti-MyoD Antibody, #ZRB1452, Sigma-Aldrich, and an anti-rabbit secondary antibody coupled with Rhodamine #SAB3700846, Sigma-Aldrich), DAPI (4′-6-diamidino-2-phenylindol ...
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bioRxiv - Cell Biology 2024Quote: ... The slides were probed with anti-PfMFS_DTHydrophillic mouse serum (1:100) and anti-PfMSP1 rabbit serum (1:300; Sigma-Aldrich) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes previously incubated with anti-RplA were incubated with the secondary antibody anti-rabbit IgG-HRP (#A8275, 1:10000, Sigma) for 1 h at room temperature and then washed three times with T-PBS ...
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bioRxiv - Plant Biology 2024Quote: ... with Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... protein isolation and immunoprecipitation was as previously described (Mohammed et al., 2016) using 1×107 OL-differentiated NSCs incubated with rabbit anti-OLIG2 (AB9610, Millipore) and using rabbit pre-immune IgG as control ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase, A3682, Sigma) were visualized using Amersham ECL or ECL Prime 225 reagent (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated overnight in primary antibody Anti-Tyrosine Hydroxylase Antibody from rabbit (1:500, cat# AB152, Sigma Aldrich) diluted in blocking solution at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Whole tissue cultures were incubated with rabbit anti-Iba1 (1:1000; Fujifilm Wako, #019–19741) or mouse anti-GFAP (1:1000; Sigma) in PBS containing 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were incubated overnight at 4°C in the presence of primary antibodies in PBS-T: rabbit PYK2 antibody (1/500, Sigma #07M4755 ...
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bioRxiv - Neuroscience 2024Quote: ... followed the same staining and mounting protocol described above but were incubated ON with a dilution of 1/2,000 anti-HA rabbit antibody (Sigma; H6908), and a 1/1000 dilution of AlexaFluor-488 donkey anti-chicken (Jackson InmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Cancer Biology 2024Quote: ... was used as the primary antibody and a goat polyclonal anti-rabbit IgG conjugated to horseradish peroxidase (Millipore, 12-348) for the secondary antibody ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... and ADP production was quantitatively coupled to the stoichiometric consumption of NADH via the activity of excess pyruvate kinase (PK, type-III from rabbit muscle, Sigma) and lactate dehydrogenase (LDH ...
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bioRxiv - Microbiology 2024Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 5% milk-PBST). Anti-ATS and anti-NapL were detected as previously described (Nacer et al ...
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bioRxiv - Microbiology 2023Quote: ... The wells were washed as before and then probed with 100µl rabbit anti-chicken IgG conjugated to horseradish peroxidase (HRP) (Sigma Ltd.) diluted 1:3000 in ELISA diluent for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and MreB or Spo0J were detected using respective in-house polyclonal antibodies and HRP-conjugated anti-rabbit IgG antibody (Sigma). Samples were developed using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with 0.05M PB containing 0.3% triton X-100 and the primary antibody (rabbit anti-FG, AB153-I, 1:10000, Sigma-Aldrich) at 4°Covernight ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with HRP-conjugated goat anti-rabbit antibody (1:10,000, Sigma-Aldrich, St. Louis, MO, USA). The signal was visualized by chemiluminescent detection using ECL.
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Physiology 2023Quote: ... sections were exposed overnight at 4°C to antibodies detecting CARNS1 (rabbit polyclonal, 1:100 in PBS with 1% BSA, HPA038569, Sigma). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were washed with PBS three times and then incubated with secondary antibodies: horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG antibodies (Millipore, Billerica ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Blots were blocked overnight in 5% milk in PBS with 0.2% Tween-20 and subsequently incubated with either rat anti-PfHSP70 (1/1000)56 or rabbit anti-PfRh5 (1/5000)57 followed by goat anti-rat HRP (Sigma) or Goat anti-rabbit HRP (Bio-rad) ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The primary antibodies used in immunofluorescence (IF) were rabbit anti-LvIRF antibody (Genecreate) and mouse anti-β-actin antibody (Sigma). The slides were washed with PBS six times and then incubated with diluted second antibodies of anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were blocked with 3% BSA in PBS with 0.3% Triton X-100 for 1h at room temperature and subsequently incubated with primary antibodies (rabbit anti-glutamate, 1:500, Sigma; rabbit anti-SOM ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... then transferred to 10 µL drops of equilibrated and pre-warmed rabbit culture medium covered with mineral oil (Sigma, M8410). Embryos were maintained in an incubator (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Expression of WT and mutant N was assessed by Western blot using a rabbit anti-N antiserum 39 and a mouse monoclonal anti-tubulin antibody (Sigma), revealed by incubation with anti-rabbit and anti-mouse antibodies coupled to HRP (SeraCare).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... and 3% BSA followed by overnight incubation at room temperature with rabbit anti-KCC2 IgG (1:1000 dilution; #07-432, Merck-Millipore) (Yassin et al. ...
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bioRxiv - Neuroscience 2023Quote: ... anti-5-HTT (guinea pig, 1:50, HTT-GP-Af1400, Nittobo Medical Co, Japan) and anti-NeuN (rabbit, 1:200, MABN140, Millipore, Burlington ...
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bioRxiv - Cell Biology 2023Quote: ... gift from Christian Ungermann) as well as respective peroxidase-conjugated secondary antibodies against mouse (dilution 1:10,000, rabbit, Sigma A9044) or rabbit (dilution 1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500 ...
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...