Labshake search
Citations for Millipore Sigma :
6701 - 6750 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-HA (rabbit monoclonal from Cell Signaling Technology Inc., cat. no. 3724S; and mouse monoclonal from Sigma-Aldrich, cat. no. H3663), anti-myc (mouse monoclonal clone 9E 10 from the University of Iowa Hybridoma Bank) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with PBS 1x-1% non-fat milk overnight at 4°C and incubated in primary antibody anti-Orco (1:500) and the secondary antibody Goat anti-rabbit IgG HRP conjugate (1:5000 Sigma-Aldrich) for 1 hour each ...
-
bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
-
bioRxiv - Bioengineering 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit IgG and visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Burlington, MA) and an ImageQuant LAS4000 digital imaging system (GE Healthcare ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were then blocked for at least 1 hour in 5% milk and then incubated overnight rocking with either rabbit polyclonal α-FLAG (1:1000, Sigma) or mouse monoclonal α-RpoA (1:1000 ...
-
bioRxiv - Biophysics 2023Quote: ... blocked in Odyssey Blocking Buffer (Licor #927-50000) at room temperature for 1 hour and incubated with primary antibodies against total Cx43 (1:5000, C6219 rabbit, Sigma Aldrich) and GAPDH (1:5000 ...
-
bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
-
bioRxiv - Genetics 2023Quote: Liver protein isolation and western blots on liver extracts and plasma samples were performed as previously described.17 Primary antibodies to the 2A peptide (1:5000, rabbit, ABS31, Sigma-Aldrich), GFP (1:3000 ...
-
bioRxiv - Cell Biology 2023Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated in primary antibody solution containing Anti-Neurofilament 200 primary antibody produced in rabbit IgG (N4142, Sigma Aldrich) and biotin-conjugated Anti-NeuN primary antibody produced in mouse IgG (MAB377B ...
-
bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
-
bioRxiv - Neuroscience 2023Quote: ... then washed with PBS again and incubated for 48h with a rabbit anti-TH antibody (1:1000, Millipore Cat# AB152, RRID:AB_390204) at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... pTyr (clone 4G10, 05-321), and HA (clone HA-7, H3663) and rabbit polyclonal anti-SEPTIN12 (HPA041128) were from Sigma-Aldrich. Rabbit and mouse monoclonal anti-DYKDDDDK (clone D6W5B ...
-
bioRxiv - Genomics 2023Quote: ... Revelation of the primary antibodies was performed by incubation with goat anti-rabbit Horseradish peroxidase-conjugated secondary antibody (Ref#A0545, Sigma-Aldrick) at 1/10000 ratio in blocking solution for 1 hour at room temperature ...
-
bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
-
bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
-
bioRxiv - Microbiology 2023Quote: PLA: PLA was carried out using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, Cat #DUO92101-1KT). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: Cytoplasmic and nuclear fractions were then subjected to western blotting and labelled with rabbit monoclonal anti-GAPDH (Sigma, G9545, 1:5,000) antibody and rabbit polyclonal anti-LSD1 antibody (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... membranes were washed four times with TBST and incubated with secondary antibody (goat-anti-rabbit or donkey-anti-mouse HRP-conjugated,1:5000 in TBST + 10% dry milk, Sigma Aldrich) for 1 hour at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... For secondary antibody incubation we used a goat-anti-guinea pig Alexa 488 (1:1000) and a goat-anti-rabbit Atto 647N (1:1000, #40839, Sigma Aldrich). After antibody incubation ...
-
bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated in primary antibody solution (1:1000 rabbit anti-GFP or 1:1000 rabbit anti-RNA Pol II CTD repeat YSPTSPS (phospho S5) in blocking buffer (1:10 Western Blocking Reagent (Sigma 11921673001) in PTx) ...
-
bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568/AttoN647-conjugated goat anti-mouse or anti-rabbit (1:100–1:400, Thermo Fischer Scientific) and Alexa 488/568-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
-
bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
-
bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
-
bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500; mouse anti ß-actin, Sigma, 1:7500 ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were then blocked for at least 1 hour in 5% milk and then incubated overnight rocking with either rabbit polyclonal α-FLAG (1:1000, Sigma) or rabbit polyclonal α-mCherry (1:10,000 ...
-
bioRxiv - Biochemistry 2023Quote: Duolink proximity ligation assays were performed using a Duolink™ In Situ PLA Kit Goat/Rabbit (Olink Biosciences) purchased from Sigma. Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... rat monoclonal antibody against the N-terminus of DAT (MAB369) and rabbit polyclonal antibody against tyrosine hydroxlase (TH) (AB152) were from EMD Millipore, Rabbit polyclonal antibodies against vacuolar protein sorting-associated protein 35 (VPS35 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
-
bioRxiv - Physiology 2023Quote: ... Collected adrenal sections were probed with antibodies for TLQP-21 (rabbit anti-TLQP-21, 1:100, 20) or Tyrosine Hydroxylase (sheep polyclonal TH, 1:100, Millipore Sigma) overnight at 4°C in a humidified chamber ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
-
bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Anti-rabbit secondary antibody conjugated with horse radish peroxidase enzyme was used in the ratio of 1:10000 (Sigma Aldrich, USA) and blots were visualized using chemiluminescent substrate as described above.
-
bioRxiv - Physiology 2023Quote: ... in PBS (BSA/PBS-T) for 1 hour prior to washing with PBS and incubation with primary anti-TH rabbit polyclonal antibody (EMD Millipore) at 1:300 BSA/PBS-T overnight at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... A secondary antibody was incubated at room temperature for 1 hour (Donkey anti-rabbit IgG antibody: Sigma-Aldrich, SAB3700932; 1:100). Fusion protein ProteinA-Tn5 was prepared in-house (plasmid Addgene #124601 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were immunostained with rabbit anti-SON antibody (1:200 dilution, Novus) or mouse anti-SRRM2 antibody (1:200 dilution, Sigma Aldrich) for 1h at room temperature followed by 3 times wash with DPBS ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were stained for c-Fos using rabbit anti-c-Fos antiserum (ABE457; Millipore, 1:1000; 1% NDS; 0.3% TxTBS) with Alexa Fluor 488 conjugated donkey anti-rabbit antiserum (AF488 ...
-
bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
-
bioRxiv - Neuroscience 2023Quote: ... Processing of brain and brain sections for TH immunohistochemistry was similar to the protocol described above except for primary (rabbit anti-TH antibody 1/200, Millipore ab152) and secondary (Alexa Fluor 555-conjugated donkey anti-rabbit ...
-
bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
-
bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation with an anti-EMILIN-1 rabbit polyclonal antibody (x400, HPA002822; Sigma Aldrich, Saint Louis, MO, USA) in Dako REAL Antibody Diluent at 4°C for 24 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...