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Citations for Millipore Sigma :
6351 - 6400 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-synaptophysin (1:500; SMC-178D, StressMarq Bio.) and rabbit monoclonal anti-GluR1 (1:500; 04-855, Millipore). Secondary antibodies were Alexa Fluor 488-donkey anti-mouse IgG (1:300 ...
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bioRxiv - Physiology 2020Quote: ... the membrane was incubated with rabbit anti-Urn8R (1:1000) and mouse anti-tubulin (1:2500; Sigma-Alrich, MO, USA) in blocking buffer supplemented with 0.2% Tween 20 (w/v) ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200; Acta2, mouse, Sigma, 1:800 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The grids were washed once with 1% BSA in DPBS and incubated with a 1:10 dilution of rabbit anti-c-Myc antibody (Sigma) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Non-infected and Lm-infected HUVEC were incubated for 1 h with primary antibody rabbit anti-STAB-1 (1:100, Millipore), diluted in 0.2% saponin (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (ref. 06570, 1:1000) were obtained from Millipore. The anti-GFP mouse monoclonal antibody mix (ref ...
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bioRxiv - Cancer Biology 2021Quote: ... The sections were incubated for 60 minutes at room temperature with the rabbit antibody PRSS2 (Center) (Sigma-Aldrich, SAB 1307060) diluted 1:50 in antibody diluent with background reducing components (DAKO S 3022) ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C with rabbit anti-myc (1:250; Sigma-Aldrich Cat# C3956, RRID:AB_439680) in PBS with 2% BSA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TN) and the secondary antibody HRP-conjugated anti-goat IgG from rabbit was purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then washed again and incubated with horseradish peroxidase-conjugated rabbit anti-horse IgG (1:1000; Sigma, UK) for 3 hours at room temperature ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... Tubulin as loading control was detected with anti-tubulin (1:10000, MBL) and HRP-conjugated anti-rabbit IgG as the secondary antibody (1:10000, Sigma). For detecting the heperphosphorylation of Crt1-13MYC ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked (for 1 hr) and incubated for 1 hr with primary rabbit anti-FLAG antibody (Sigma-Aldrich). Following buffer wash the membrane was incubated for 1 hr with HRP-conjugated IgG mouse anti-rabbit antibodies (ThermoFisher Sci.) ...
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bioRxiv - Physiology 2021Quote: ... Muscles were then incubated in blocking solution containing rabbit anti-heavy NF200 primary antibody (N4142 Sigma, Germany; 1:200 dilution) and anti-synaptophysin antibody (1:25 dilution as above) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Sections were stained for NeuN immunohistochemistry using 1:10,000 polyclonal rabbit anti-NeuN antibody (ABN78; EMD Millipore; Temecula, CA, USA) and horse anti-rabbit secondary antibody via an HRP reagent (ImmPRESS MP-7401 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4 °C with the first antibody (rabbit anti-Tc-Zen1 and anti-Tc-Zen2 as for immunohistochemistry, 1:1,000; mouse anti-Tubulin [Sigma-Aldrich #T7451 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in PBS containing 0.3% Triton X-100 and 10% normal donkey serum and incubated with primary antibody overnight at 4 °C overnight (Rockland, rabbit anti RFP, 1:500; Millipore, chicken anti-GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... VP sections near the center of the microinjection sites were incubated overnight at room temperature in rabbit anti-Fos (1:5000; Millipore) and mouse anti-DSRed (targeting mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... fixed with a 4% PFA solution and stained for tyrosine hydroxylase (TH; rabbit anti-TH, 1:1000, Cat#: 657012, Merck Millipore) and neurobiotin (Streptavidin Alexa Fluor conjugate 647 ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibodies at the indicated dilutions were employed in western blot analysis: rabbit polyclonal anti HA (SIGMA, 1:3000), mouse monoclonal anti HA (Cell Signalling ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared extract was adjusted to 0.5% Triton X100 and 0.1% Tween 20 and added to magnetic beads prepared from 400 microlitres of an IgG (rabbit serum, I5006-100MG, Sigma)-coupled magnetic beads slurry (1 mm BcMag ...
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by incubation with a secondary Cy3-labeled polyclonal goat anti-rabbit antibody (AP132C, 1:800, Merck Millipore, Burlington, USA). Cells were mounted on slides using Fluoroshield™ with DAPI mounting medium (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:1000 in phosphate-buffered saline (PBS) containing 0.05% Tween 20 (PBS-T) followed by anti-rabbit horseradish peroxidase (HRP)-coupled antibody (Sigma-Aldrich) diluted 1:1000 in PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... lysates were pre-cleared for 1 hour (h) with mouse immunoglobulin G (IgG) agarose or rabbit immunoglobulin G (IgG) agarose (Sigma), dependent on the species of antibody used in IP ...
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bioRxiv - Immunology 2021Quote: ... All primary antibodies were purchased from Cell Signalling Technology (Singapore) except for rabbit anti-MLC phospho-Ser19 (Sigma Aldrich, Singapore), rabbit monoclonal anti-detyrosinated tubulin and phospho-tubulin Tyr272 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... The expression of tagged MCM2 in HeLa and Patski cells was confirmed by Western blot using anti-MCM2 rabbit polyclonal antibody (HPA031495 Sigma) at 1:1,000 dilution.
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Genetics 2020Quote: ... 4 RIMBP1 WT and 4 RIMBP1 KO cerebelli were cut parasagittal (30 um) and stained with antibodies anti-GAD65 (1:1000, mouse, DSHB) and anti-calbindin (1:1000, rabbit, Millipore). Synaptic puncta were imaged using a Nikon confocal microscope (A1RSi+ ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... washed and incubated with HRP-conjugated goat anti-rabbit secondary antibody and developed using enhanced chemiluminescence substrate (Millipore, ON, Canada). Membrane was stripped using 0.1 N NaOH and reprobed with rabbit anti-beta actin antibody (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in 5% NGS with 0.1% Triton X-100 and incubated with anti-NECAB1 rabbit antibody (Sigma Millipore #HPA023629) and anti-NeuN (Thermofisher Scientific #14H6L24 ...
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bioRxiv - Microbiology 2022Quote: ... 0.1% Tween-20 with 5% w/v nonfat dry milk) and incubated with diluted (1:10.000) rabbit polyclonal antiserum (Sigma-Aldrich) against mCherry overnight ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4% Triton and the rabbit anti-calbindin-D-28K primary antibody (C7354; Sigma-Aldrich St. Louis, MO, USA; 1:1000). Sections were subsequently washed in PBS ...
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated with PBS + 1% milk solution containing a 1:10.000 anti-rabbit horseradish peroxidase conjugated secondary antibody (A0545, Sigma-Aldrich). The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated for 5 min with Pierce ECL Plus substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Immunology 2022Quote: ... The membrane was then washed three times and incubated for one hour with HRP-conjugated goat anti-rabbit IgG (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Pathology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
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bioRxiv - Plant Biology 2022Quote: ... followed by incubation in a 1:10000 dilution of the fluorescein Dylight 405 labelled goat anti-rabbit secondary antibody (Sigma) in PBS-BSA for 2 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... we confirmed targeting of the optic-fiber-tetrode bundle to LC by colocalization of the electrolytic lesion with immunostaining against tyrosine hydroxylase (rabbit anti-TH, ab152, Millipore, followed by donkey anti-rabbit Alexa 488 ...