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Citations for Millipore Sigma :
6601 - 6650 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804; rabbit anti-NF-145 – Millipore AB1987), followed by washing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were Immunoblotted with incubation of anti-Ahnak (rabbit polyclonal, RU2024, 1:5,000) or Anti-Gapdh (mouse monoclonal, MAB374, Millipore, 1:5,000) primary antibody followed by incubation of horseradish peroxidase-linked goat anti-rabbit or anti-mouse secondary antibody (1 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots were probed with the following antibodies at the indicated concentrations: rabbit polyclonal anti-FLAG (Millipore Sigma, F7425; 1:1,000), mouse monoclonal anti-FLAG M2 (Millipore Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins transferred to nitrocellulose membranes were probed with either affinity-purified polyclonal rabbit anti-Gli326 or goat polyclonal anti-Gli3 (R&D, AF3690) and mouse anti-vinculin (Sigma, V9264) and visualized with fluorescent secondary antibodies (LI-COR IRDye 800CW ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with the primary antibodies (Abs): 1:400 rabbit anti-GFAP (EMD Millipore catalog#ABS5804); 1:1000 rabbit anti-Iba1 (Wako catalog#019-19741) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2 ...
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bioRxiv - Immunology 2021Quote: ... antibody diluted 1:1000 in blocking solution or rabbit Anti-Monkey IgG (whole molecule)-Peroxidase antibody (Sigma-Aldrich Cat# A2054, RRID:AB_257967) were added to each well ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunocytochemistry using primary polyclonal antibodies of phosphorylated Tau pSer214 (, rabbit, 1:1000 Sigma, USA) and NOS (Cayman Chemical,1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... Guinea pig anti-vesicular glutamate transporter 2 (VGluT2) (Cat#2251-I; RRID: AB_2665454) and Rabbit anti-NeuN (Cat# MAB377; RRID: AB_2298772) was purchased from Millipore (Temecula, CA, USA). Rabbit anti-PAX6 (Cat#901301 ...
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bioRxiv - Developmental Biology 2021Quote: ... the membrane was then incubated overnight at 4°C with primary antibodies: 1∶15000 rabbit anti-V5 antibody (SIGMA, Cat. # V8137), and 1:12000 mouse anti-α-Tubulin antibody (SIGMA ...
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bioRxiv - Immunology 2020Quote: ... the primary antibodies were discarded and the sections probed with secondary antibody (anti-rabbit IgG conjugated to FITC) (Sigma-Aldrich, Germany) prepared at a dilution of 1:10000 in PBST ...
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bioRxiv - Neuroscience 2021Quote: The following primary antibodies were used for staining: anti-phospho-CREB (ser133) antibody (1:1000 dilution, rabbit, Millipore Sigma, 06-519), Phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 x 10 min in PBS-TX (0.2% Triton X-100 in 0.1M PBS) and incubated with the primary antibody rabbit anti-CTB overnight (1:3000) (Sigma-Aldrich, UK). Sections were washed for 3 x 10 min (PBS-TX ...
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bioRxiv - Microbiology 2020Quote: ... The heat inactivated sera were serially diluted in BSK-II media without rabbit sera followed by being mixed with complement-preserved sera from guinea pig (Sigma-Aldrich) or chicken (Biowest ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized with 0.3% Triton X-100 in PBS (PBST) for 10 minutes and incubated with rabbit primary antibody against TH (1:1000, AB152; Millipore/Sigma) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then blocked for 1h in PBST with 5% of Bovine Serum Albumin (BSA) and incubated overnight with rabbit anti-TH antibody (1:1000, Millipore, AB152) diluted in PBST with 2% of BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The following day plates were washed three times with TBS-T and incubated for 2 hours at room temperature with secondary antibody horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma A6917), diluted to 1 in 1,000 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: Blots were washed three times for 15 minutes with PBS-T and probed 45 minutes with secondary antibodies (rabbit anti-mouse (Sigma A4312), 1:10000 dilution ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Cell Biology 2022Quote: The staining of cells and tissues was performed with the following primary antibodies: rabbit polyclonal anti-CEP164 (1:1,000; EMD Millipore, ABE2621), mouse monoclonal acetylated-α-tubulin (1:1,000 IF ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore; 1:300 ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated in TBST-5 % w/v milk (TBST supplemented with 5 % (w/v) skimmed milk powder) with anti-rabbit-HRP secondary antibody (A0545, Sigma Aldrich) (1:5,000 ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C and followed by 1-h incubation at room temperature with HRP-conjugated anti-rabbit secondary antibody (1:2000, Sigma Aldrich). Chemiluminescence was detected using ECL Western Blotting substrate (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were incubated at room temperature for 1 h with Cy2/Cy3-conjugated anti-mouse or anti-rabbit secondary antibody (1:50; Millipore AP182C). After three washes in PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... dishes were washed twice for 5 minutes with 0.002% Triton X-100 in PBS and incubated with anti-rabbit-PLUS (Sigma-Aldrich, DUO92002) and anti-mouse-MINUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... the ligation experiment was performed according to the manufacturer’s instructions using the Duolink® In Situ Orange Starter Kit Goat/Rabbit (Sigma-Aldrich). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with the rabbit anti-NeuN (1:800, Beyotime Biotechnology) or monoclonal anti-Dicer 1(1:300, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... before being rinsed twice for 15 min each in PBS/BSA and incubated for 15 min in a 1/100 dilution (in PBS/BSA) of biotinylated goat anti-rabbit secondary antibody (Sigma Aldrich). Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to a PVDF membrane and incubated with one of the following primary antibodies: α-FLAG polyclonal rabbit (Sigma) or α-RpoA monoclonal mouse (Biolegend) ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit antibody conjugated to peroxidase (Sigma Aldrich). For tagged proteins detection ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP, Sigma, #A9037) at a 1:5000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-FLAG clone M2 (WB 1:4000, IF 1:400; F1804) and rabbit anti-LC3B (WB 1:1000, IF 1:200; L7543) were from Sigma-Aldrich; goat anti-FLAG (1:400 ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed with TBST buffer (5 x 1 min) and probed with secondary goat anti-rabbit antibodies conjugated with peroxidase (1:1000, Sigma #A6154). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifescences #RPN2235 ...
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bioRxiv - Microbiology 2021Quote: In situ proximity ligation assay (PLA) was performed using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... The PVDF membrane was then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (diluted 1:10,000; Sigma-Aldrich, Stockholm, Sweden) for 1 h at room temperature followed by three washes with TBST and detection using a Agrisera ECL kit (catalogue no ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary antibodies (rabbit anti-phospho-ERK, Cell Signaling Technology #9101, 1:1000; mouse anti-α-tubulin, Sigma-Aldrich #T9026, 1:5000) were diluted in Odyssey Blocking Buffer containing 0.2% Tween 20 ...
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bioRxiv - Microbiology 2019Quote: For ChIP the following antibodies were used in this study: Rabbit polyclonal anti H3K9/K14-ac (Merck Millipore; Cat#06-599); Rabbit monoclonal anti H3K4-me3 (clone MC315 ...
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bioRxiv - Immunology 2019Quote: ... Beads were harvested and boiled in 40 μL of Laemmli sample buffer prior to western blot analysis with a polyclonal rabbit anti-PTPN2 antibody (SAB4200249, Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Pathology 2021Quote: ... cryosections were transferred to TBS and incubated for 1 h at 37 °C with anti-mouse PLUS and anti-Rabbit MINUS PLA probes (Sigma-Aldrich). Then ...