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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with anti-HA antibody and immobilized on protein A Sepharose beads (Sigma-Aldrich). Beads washed 3 times with lysis buffer and then washed twice with kinase buffer (1xPBS (pH7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... the membranes with proteins were incubated with primary antibodies diluted in 4% bovine serum albumin (BSA [Sigma, A7030] in TBST containing 0.02% sodium azide [Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The FLAG fusion protein was immunoprecipitated with an anti-FLAG antibody conjugated with M2 beads (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... which were then plated in black 96-well plates in the presence of 5 mM complex I substrates glutamate and malate with or without 5 µM CCCP (carbonyl cyanide m-chlorophenyl hydrazone, Sigma-Aldrich) and 1 µM TMRM (tetramethylrhodamine ...
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bioRxiv - Microbiology 2021Quote: We resuspended wild-type and mutant yeast cells in 50 mM potassium phosphate buffer to a final concentration 5 × 107 cells/ml and supplemented the suspension with filipin (filipin complex from Streptomyces filipinensis; Sigma F9765) to a final concentration of 5 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... High Five™ insect cells for the expression of PP2A A/C complex were cultured in EX-CELL 405 Serum-Free medium (Millipore-Sigma) at 27°C with 130 rpm ...
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bioRxiv - Immunology 2020Quote: ... Agarose was pelleted and placed at rotation for five minutes with Low Salt Immune Complex Wash Buffer (Millipore, Cat #20-154), then pelleted ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... Samples of PARP-1 CAT domain in complex with PARP inhibitors for NMR were made by adding inhibitors (50mM in [2H6] DMSO, Sigma-Aldrich) to a final ratio of 1:1.5 (veliparib ...
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bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Developmental Biology 2020Quote: ... Four to six larval body wall tissues containing muscle-epidermis complex were homogenized in 21 μl of RIPA buffer (Sigma, #R0278) supplemented with a cocktail of protease inhibitors (Complete mini ...
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bioRxiv - Cell Biology 2021Quote: ... Replenishment of cholesterol was performed by incubating the cholesterol-depleted cells in 10 mM MβCD-cholesterol complex (1:1 mol/mol; Sigma Aldrich: C3045) at 37°C for 30 min38 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed by dounce homogenization and complex formation was initiated with the addition of 25 mU/mL Apyrase (Sigma-Aldrich) and 10 μM ET-1/ IRL1620 (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... TAP- and HTP-tagged proteins were detected after a unique incubation of the membranes with Peroxidase Anti-Peroxidase Complex produced in rabbit (1:2,000; Sigma, Cat# P1291). Chemiluminescence signals were revealed on an Amersham™ Imager 600 (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: Oligomycin and antimycin A (inhibitor of mitochondrial complex V and III respectively) were purchased from Sigma-Aldrich (O4876 and A8674 respectively).
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bioRxiv - Cell Biology 2024Quote: ... a complex of siRNA and DharmaFECT 2 was prepared in serum-free DMEM (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) at a volume of 100 μL/well according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Then 0.4 µL of the secondary-readout (S-RO) complex was diluted in 200 µL EC buffer [10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911) ...
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bioRxiv - Cell Biology 2022Quote: Drugs used in this study include the Arp2/3 complex inhibitor CK-666 (48) and the formin agonist IMM-01 (49) and were purchased from Sigma-Aldrich. For drug treatments prior to immunoprecipitation experiments ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
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bioRxiv - Cell Biology 2024Quote: ... such as pyruvate and malate entering the chain at complex I) was measured after the addition of 5 mM pyruvate (P2256, Sigma-Aldrich) + 2 mM malate (M0875 ...
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bioRxiv - Physiology 2024Quote: ... Polyclonal anti-5-HT was raised in rabbit against a 5-HT creatinine sulfate complex conjugated with bovine serum albumin (manufacturer specifications; cat. no. S5545, Sigma-Aldrich, Oakville ...
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bioRxiv - Cell Biology 2024Quote: ... a complex of siRNA and DharmaFECT 2 was prepared in serum-free DMEM (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) at a volume of 100 μL/well according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts from SCN cultures were examined by SDS-PAGE immunoblotted with anti-UBE3A monoclonal antibody (E8655, Sigma), or anti-FLAG monoclonal antibody (F1804 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein levels of wild-type or mutant Rad6 were determined by immunoblotting using anti-Flag M2 antibody (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-parvalbumin (PV;P3088) and anti-glial fibrillary acidic protein (GFAP;G3893) antibodies were obtained from Sigma-Aldrich; bromodeoxyuridine (BrdU ...
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bioRxiv - Microbiology 2021Quote: ... MERS S-protein was detected using a monoclonal anti-FLAG M2-HRP conjugated antibody (SIGMA, A8592 @ 1:2000) which bound to a C-terminal FLAG-tag ...
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bioRxiv - Immunology 2020Quote: For immunoprecipitation experiments the Chk1 or GATA3 antibody was incubated with Red Protein G Affinity beads (Sigma-Aldrich) for 1 hour at 4°C on a shaker to allow the antibody to bind to the beads ...
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bioRxiv - Physiology 2020Quote: ... and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000, Sigma, #S8809), the anti-transferrin receptor mouse monoclonal antibody (TransR ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then analyzed by western blotting using a primary anti-biotin antibody (1:5,000 dilution; Sigma-Aldrich) and an anti-mouse secondary antibody coupled to peroxidase (1:10,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... the separated proteins were transferred to nitrocellulose and probed with commercial primary antibodies recognizing FLAG (Sigma, cat# F3165), HA (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Input lysates and pulldown proteins were analyzed by western blotting with the anti-FLAG M2 monoclonal antibody (Sigma) and anti-GAPDH monoclonal antibody (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to PVDF membranes and probed with M2 anti-FLAG antibody (Sigma; 1 in 5,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Neuroscience 2022Quote: ... and immunoblotted for protein expression using the following antibodies: guinea pig anti-GLT1 at 1:25,000 (Millipore AB1783) and mouse anti-beta actin at 1:20,000 (Sigma-Aldrich A1978) ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (A3854, Sigma-Aldrich) and imaged as above.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins (0.5-1 mg) were precipitated using the anti-FLAG antibody (1:100, Cat. #F7425; Sigma-Aldrich), anti-HA antibody (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... and proteins were detected by western blotting using either Anti-FLAG M2 monoclonal antibody-peroxidase conjugate (Sigma, A8592) at a dilution of 1:10000 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to PVDF membrane and probed with M2 anti-FLAG antibody (Sigma; 1 in 1,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto a Protran nitrocellulose membrane and probed with antibody clone 12D10 against puromycin (Millipore MABE343). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Biochemistry 2021Quote: ... and the immunoprecipitated proteins were subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-71 [40] anti-S protein antibodies and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...