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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Physiology 2022Quote: ... protein G beads were coupled to the pre-pro-vasopressin antibody or normal rabbit serum (Sigma) at a 1:50 ratio for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The immunoprecipitation was performed by incubating the protein lysate with 1ug of GluA1 antibody (Millipore, ab1504) or 1ug HA antibody (Santa Cruz ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Puromycin incorporation into neosynthesized proteins was assessd with Western blotting and an anti-puromycin antibody (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449), secondary antibody (Biorad #170-6515) ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation was carried out with β-catenin monoclonal antibody and Protein G magnetic beads (Sigma-Aldrich). For PCR ...
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bioRxiv - Cell Biology 2022Quote: ... The same membrane was stripped of antibody and total protein was determined with Coomassie (Sigma, B0149) staining ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using chemiluminescence from horseradish peroxidase-coupled secondary antibodies Anti-rabbit HRP (Sigma; A0545), 1:15,000.
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bioRxiv - Bioengineering 2022Quote: ... Protein bound to the wells were detected with a peroxidase conjugated anti-Flag antibody (Sigma A8592) and quantified with QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™ 15169) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50µL of protein G beads were coated 2ug of anti-AGO2 antibody (Sigma SAB4200085 Lot# 0000089486) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies to the following proteins were used in the immunoblots: puromycin (Millipore, #AF488, 1:2000; RRID:AB_2737590), 4E-BP1 (Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extract (200 μg) was incubated with 1 μL polyclonal anti-HA antibody (Sigma-Aldrich H6908) in immunoprecipitation buffer for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The expressed protein was confirmed by Western blot analysis using anti-FLAG-tag antibodies from Sigma. The centromeric repeat pAL1 was amplified from Arabidopsis Col-0 genomic DNA using IRD700 labeled and unlabeled oligos ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with anti-HA antibody and immobilized on protein A Sepharose beads (Sigma). Beads washed 3 times with lysis buffer ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Developmental Biology 2024Quote: ... Another set of Dynal Protein G Magnetic beads was coupled to anti-FLAG antibody by incubating washed Protein G beads with anti-FLAG antibody (Sigma Aldrich; Catalog number: F1804) with end-over-end rotation for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Physiology 2021Quote: ... 500 μg of lysates/mouse were then immunoprecipitated with anti-PKG antibody(19) and protein conjugated-Sepharose beads and blotted with anti-NT antibody (Millipore, Cat#05-233, 1:2000). The same membrane was also blotted with anti-PKG antibody as a loading control ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for immunofluorescence (IF) in this study: a monoclonal antibody (mAb) against tau protein (clone Tau-1; Millipore, Cat Number: MAB3420, RRID: AB_94855) diluted 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the complex were pooled and concentrated to ~34mg/mL in a 10kDa molecular weight cutoff concentrator from Millipore.
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Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membranebioRxiv - Cell Biology 2020Quote: ... The fixed cells were washed twice with phosphate-buffered saline (PBS) and resuspended in PBS containing 2.5 mg/mL filipin complex (Sigma-Aldrich). After incubation at room temperature for 15 min in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted complexes were fractionated by 10%–30% glycerol gradient and the fractions containing intact BAF complex were combined and concentrated using Amicon Ultra-4 centrifugal filter (Millipore).
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bioRxiv - Bioengineering 2021Quote: ... 200 mg Ru(dpp)3(ClO4)2 complex was dissolved in 10ml ethanol (ACS reagent ≥99.5%, CAS 459844; Sigma-Aldrich). Silica gel was prepared by adding 2 g silica particles to 40 ml aqueous NaOH (0.01N ...
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bioRxiv - Biochemistry 2020Quote: Complex concentrations were estimated using quantitative western blotting against γ-tubulin standards using anti-γ-tubulin (clone GTU-88; Sigma), assuming that each complex contains 14 γ-tubulin molecules ...
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bioRxiv - Biophysics 2021Quote: ... The monomeric complex peak was collected and concentrated to ~5 mg/ml with a 100 kDa cut-off concentrator (Millipore), and then analyzed by SDS-PAGE and analytical size-exclusion chromatography.
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bioRxiv - Plant Biology 2020Quote: ... Mucilage immunolabeling with CCRC-M139 (Carbosource, Complex Carbohydrate Research Center) and counter-staining with S4B (Direct Red 23; Sigma Aldrich) was performed using a published protocol and Leica TCS SP8 confocal setup (Voiniciuc ...
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bioRxiv - Microbiology 2021Quote: ... The deposited C5b9 complex in the reaction mixture was detected using rabbit anti-C5b-9 pAb at 1:1000 dilution (204903; Sigma) and HRP conjugated anti-rabbit pAb (1:5000 ...
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bioRxiv - Biophysics 2021Quote: ... The purified AVP–V2R–Gs complex was concentrated using a Amicon Ultra centrifugal filter (molecular weight cut-off of 100 kDa, Millipore) and then subjected to a Superdex 200 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were kept in CSK buffer with 0.5% Triton X-100 and 2 mM VRC (vanadyl ribonucleoside complex, Sigma-Aldrich) for 10 min on ice and were treated with 4% paraformaldehyde solution for 8 min of fixation ...
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bioRxiv - Neuroscience 2022Quote: ... High Five™ insect cells for the expression of PP2A A/C complex were cultured in EX-CELL 405 Serum-Free medium (Millipore-Sigma) at 27°C with 130 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... The incubated RdRp complex was then concentrated with a 100 kDa molecular weight cut-off centrifugal filter unit (Millipore Corporation) and then further purified by size exclusion chromatography using a Superdex 200 10/300 GL column in 25 mM TrisHCl pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... The contribution of complex I to the OXPHOS respiration was achieved by addition of 10 mM glutamate (Sigma Aldrich, G1626) in presence of a saturating concentration of ADP (2.5 mM ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM β-mercaptoethanol and 5% glycerol was mixed gently with 20 µL of 1 M dimethylamine-borane complex (Sigma) per 1 mL of protein solution ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was concentrated to 30mg/ml with Amicon centrifugal filter units with 100 kDa molecular weight cutoff (Sigma Millipore).
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Biochemistry 2020Quote: ... and those containing SAM complex were pooled and concentrated using 100kDa molecular weight cut off Amicon Ultra Centrifugal Filter Unit (Millipore).