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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 500 µg of protein was incubated overnight at 4°C with primary antibody as indicated (anti-AHCY [MBL RN126PW], anti-HA [Millipore 05-904] ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were sequentially incubated with primary and HRP-coupled secondary antibodies and proteins were detected using chemiluminescence reagents (Luminata Crescendo, Millipore) and X-ray films ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and nsp12 proteins were subsequently detected using the mouse monoclonal anti-DYKDDDDK M2 antibody (Catalogue number F3165, Sigma-Aldrich) diluted 1:1000 in TBST/5% milk ...
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bioRxiv - Cell Biology 2021Quote: ... 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma) were added to each sample for 3.5 hours at 4°C after the overnight incubation with antibody ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated with 50 ml of washed Protein G Dynabeads pre-coated with 1mg of S9.6 antibody (Millipore MABE1095) for 16h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was assessed 72 hours post transfection via Western Blot using a monoclonal antibody against the FLAG epitope (Sigma) (Figure 5A ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS–PAGE gels and detected using immunoblotting with a monoclonal α-Flag antibody (Sigma-Aldrich) or a α-GFP antibody (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Cell Biology 2022Quote: ... β-actin protein level was evaluated by western blot analysis of rehybridized membranes with anti-β-actin antibody (Sigma, A5441) at a 1:5000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... After blocking samples were incubated for 120 hours with primary antibody or trimeric RBD protein in PBSGT with 0.1% saponin (S2149, Sigma-Aldrich) on a shaking incubator at 200 rpm ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature with an antibody against mHTT protein (1-82 aa, Millipore, MAB5492 1:500), and either NeuN (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µg of water-resuspended antibody was used to bind to 30 µl of PureProteome Protein A magnetic beads (Millipore).
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of extracted protein was transferred to Eppendorf tubes to incubate with anti-FLAG-antibody (1:1000; Millipore Sigma) or anti-HIS-antibody (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Purified immunoglobulin (Ig) was purified using an antibody purification system based on protein A-affinity chromatography (PURE-1A; Sigma-Aldrich). The manufacturer’s instructions were as described in [8] ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Lysates were collected by centrifugation and 500 μg of protein were incubated with 0.5 μl of Flag antibody (#F3165, Sigma-Aldrich) at 4 ℃ overnight ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with primary antibodies against protein of interest (NPR, Santa Cruz, sc-39008, 1:100; GFP, Aveslab, GFP-1020, 1:5,000; FLAG, Sigma, F1804 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...