Labshake search
Citations for Millipore Sigma :
351 - 400 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Impact of DNA sequences in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complexbioRxiv - Biochemistry 2020Quote: ... Purified crosslinked complex eluted at 400–480 mM NaCl and was further concentrated by ultrafiltration (Amicon, Millipore) to ∼3.5 mg/ml (30 μM).
-
bioRxiv - Molecular Biology 2024Quote: ... The complex was purified using its incubation with 1 ml of anti-FLAG M2 affinity gel (Sigma) 6h at 4CC ...
-
bioRxiv - Genetics 2024Quote: ... were lysed with 175 μl of cold cytoRNA solution (50 mM Tris HCl pH 8.0; 140 mM NaCl; 1.5 mM MgCl2; 0,5% NP-40; 2mM Vanadyl Ribonucleoside Complex; Sigma) and incubated 5′ in ice ...
-
bioRxiv - Biophysics 2023Quote: ... The purified NaV1.6EM complex was eluted with buffer D (buffer C plus 5 mM desthiobiotin (Sigma, USA)) and was subsequently concentrated to 1 mL using a 100 kDa cut-off Amicon ultra centrifugal filter (Merck Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat#479543), 5 µL of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat# 479543), and one drop of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8 -tetravinylcyclotetrasiloxane (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were lysed with 175 μl of cold cytoRNA solution (50 mM Tris HCl pH 8.0, 140 mM NaCl,1.5 mM MgCl2, 0,5% NP-40, 2mM Vanadyl Ribonucleoside Complex; Sigma) and incubated 5 min on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... Pelleted nuclei were extracted with 175 μl of cold nucRNA solution (50 mM Tris HCl pH 8.0, 500 mM NaCl, 1.5 mM MgCl2, 0,5% NP-40, 2mM Vanadyl Ribonucleoside Complex; Sigma) and incubated 5 min on ice ...
-
bioRxiv - Biochemistry 2024Quote: ... each complex was expressed from a polycistronic vector (Table 2) in Rosetta 2 DE3 pLysS cells (Novagen). Cells were grown to OD600 = 0.6 and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
-
bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... The membrane was cut across the molecular mass region of the corresponding proteins and separately probed with the following corresponding antibodies: an anti-β-glucuronidase (N-terminal) antibody (G5420, Sigma‒Aldrich) and an anti-tubulin antibody (T5168 ...
-
bioRxiv - Cell Biology 2020Quote: ... Antibodies against cytoskeletal proteins (vimentin, actin and β-tubulin were from Sigma (St.Louis, MO, USA). The mitochondrial marker MitoTracker® ...
-
bioRxiv - Genetics 2020Quote: ... Chromatin-bound antibodies were recovered with 30μl Protein A/G magnetic beads (Millipore 16-663). After reverse crosslinking ...
-
bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibody against HCoV-OC43 nucleocapsid (NP) protein was purchased from Millipore (Cat.No. MAB9012). Rabbit polyclonal antibody against human LY6E was obtained from proteintech (Cat.No ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
-
bioRxiv - Microbiology 2021Quote: ... was used for blotting and proteins were stained using the following antibodies: (Sigma-Aldrich AV46863), TMEM41B (Sigma-Aldrich HPA014946) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The following commercially available antibodies were used to detect targeted proteins: anti-SPF45 (Sigma-Aldrich), anti-SF3b155 (MBL Life Science) ...
-
bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
-
bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
-
bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
-
bioRxiv - Cell Biology 2020Quote: The following proteins were detected by western blotting (antibodies in brackets) CPSF3 (Sigma-Aldrich, HPA04657), IntS11 (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000 ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
-
bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antibodies were purified from hybridoma culture supernatant using protein G Sepharose 4 Fast Flow (Sigma). Injection of the respective antibody resulted in depletion of >90% of CD4+ or CD8+ T cells for at least 14 days ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP) (EMD Millipore; AB5804), rabbit anti-microtubule associated protein-2 (MAP2 ...
-
Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
-
bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
-
bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
-
bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following proteins were purchased from the indicated vendors: MAP2 (EMD Millipore AB5622); SLC17A6 (Alomone Labs AGC-036-GP) ...
-
bioRxiv - Plant Biology 2023Quote: ... was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma, A6154).
-
bioRxiv - Pathology 2023Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
-
bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized with the FITC- and TRITC-conjugated secondary antibodies (Sigma, F6005, and T5393) for 1 h at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... Proteins reacting with primary antibodies were visualized by the Immobilon forte western HRP substrate (Millipore) for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).
-
bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
-
bioRxiv - Cell Biology 2022Quote: ... and the lysates were immunoprecipitated with specific antibody and protein A/G-Sepharose (Sigma-Aldrich) at 4 °C for 2 h ...
-
bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
-
bioRxiv - Plant Biology 2023Quote: ... antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma) antibody for AVR-Pii and ZiF effectors in pGADT7.
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were detected by Western blotting using the following primary antibodies against: Flag (M2, Sigma), ERK1/2 (3A7 ...
-
bioRxiv - Plant Biology 2023Quote: ... The AGO1 protein was detected by Western blot using a Flag-HRP antibody (A8592, Sigma).
-
bioRxiv - Neuroscience 2023Quote: ... We used primary antibodies for astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541). To quantify relative intensity of staining for GFAP we measured the average signal intensity in the region of interest and normalized it to that of the WT littermate mouse ...
-
bioRxiv - Molecular Biology 2024Quote: ... the soluble antibody-bound fraction was incubated with salmon sperm protein A agarose beads (Millipore). For GFP-STK19 or CSB-mScarlett IPs the soluble fraction was incubated with 25 μl ChromoTek GFP-or RFP Trap® Agarose (chromotek ...
-
bioRxiv - Microbiology 2024Quote: ... Monoclonal antibodies against the following proteins and epitopes were used: β-actin (AC-74; Sigma); HA (3F10 ...
-
bioRxiv - Biochemistry 2024Quote: ... SPO11 protein was immunodetected using an anti-Flag-HRP monoclonal antibody (mouse, 1:1000, Sigma), followed by incubation with ECL Prime western blotting detection reagent (Amersham ...
-
bioRxiv - Genetics 2024Quote: ... Protein extracts from transfected cells were analyzed by western blotting using commercial primary antibodies (Sigma anti-FLAG M2 F1804 ...