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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The sample was crosslinked to increase the amount of complex formed by addition of 0.0125% (v/v) glutaraldehyde (Sigma-Aldrich), at room temperature for 15 min before quenching with 200 mM Tris pH 7.4 (final concentration) ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the nanodisc-embedded complex were pooled and concentrated on a 30 kDa cut-off ultra-filtration device (Amicon Ultra, Merck Millipore). For the reconstitution of radioactive nanodisc-embedded MlaF(His-E)B and MlaF(His-E)DB complexes ...
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bioRxiv - Molecular Biology 2020Quote: ... washed and homogenized with a Dounce homogenizer in a buffer containing 0.25 M sucrose,78 mM KCl,4 mM MgCl2,8.4 mM CaCl2,10 mM EGTA,50 mM HEPES-NaOH pH 7.0 supplemented with 100 μg/ml of Cycloheximide 5 mM Vanadyl Ribonucleoside Complex (VRC) (Sigma Aldrich), 0.5 mM DTT and 1X Protease Inhibitor ...
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bioRxiv - Immunology 2021Quote: ... dermatitidis ATCC strain 26199 was used for this study and grown as yeast on Middlebrook 7H10 agar with oleic acid-albumin complex (Sigma) at □ LJC.
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bioRxiv - Immunology 2020Quote: ... dermatitidis ATCC strain 26199 was used for this study and grown as yeast on Middlebrook 7H10 agar with oleic acid-albumin complex (Sigma) at 39 °C.
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bioRxiv - Evolutionary Biology 2020Quote: ... were transferred to individual wells of a 96-well microtiter plate containing 100 µL of M9 buffer (without bacterial food) containing 10 mg/mL serotonin (5-hydroxytryptamine creatinine sulfate complex, Sigma). Egg-laying rates were measured by counting the number of eggs laid after three hours in the presence or absence of serotonin at 20°C (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was concentrated to 30mg/ml with Amicon centrifugal filter units with 100 kDa molecular weight cutoff (Sigma Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
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bioRxiv - Neuroscience 2022Quote: ... Currents of 5-HT3A receptors and engineered constructs expressed at the cell surface were evoked by applying 10 μM serotonin creatinine sulfate complex (Sigma), or in short 5-HT ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-Cter-α4 or GST-β3) was mixed with 1.5 μL of active Ccnd1-Cdk4 complex purified from baculovirus (Sigma C0620), 10 mM ATP ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted complex was magnetically separated from the beads and transferred in a 384-well plate (Nunc catalog #264573; Sigma) where it was neutralized with 10 μL/well of Erenna buffer D (catalog #02-0368-00 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Developmental Biology 2024Quote: ... and added into the hybridization solution (2xSSC, 50% formamide, 10% dextran sulfate, 2 mg/ml BSA, 10 mM vanadyl ribonucleoside complex (Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were fixed with PFA+sucrose then permeabilized with PBS containing 0.25% Triton X-100 and 2mM Vanadyl Ribonucleoside Complex (VRC, Sigma Aldrich) for 10min at room temperature ...
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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryoEMbioRxiv - Immunology 2024Quote: ... SEC fractions corresponding to the complex were pooled and concentrated to 0.5 mg/ml using an Amicon filter unit with 10 kDa cutoff (EMD Millipore)
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bioRxiv - Biochemistry 2024Quote: Aliquots of 3 μl of ∼2.5 mg/ml of the NHEJ complex (based on DNA-PKcs concentration) were mixed with 8 mM CHAPSO to eliminate particle orientation bias (final concentration; Sigma) before being applied to Holey Carbon grids (Quantifoil Cu R1.2/1.3 ...
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bioRxiv - Biochemistry 2024Quote: ... The appropriate fractions were pooled and the complex was concentrated to ∼ 9 mg/ml using Amicon UltraCel 10K MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Plant Biology 2020Quote: Proteins were resolved on SDS-PAGE followed by immunoblotting using α-HA high affinity antibody 3F10 (Sigma) and α-c-Myc Antibody (9E10) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Biophysics 2020Quote: ... Separated proteins were transferred to a nitrocellulose membrane and immunoblotted with an antibody recognizing Orai1 (Sigma Aldrich). Each experiment was performed at least 3 independent times and analysed with the program ImageJ (NIH ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total protein synthesis was measured by immunoblot using the anti-puromycin antibody at 1:500 (EMD Millipore). Protein synthesis was quantified by densitometry using ImageJ and normalized by immunoblot using antibody specific to steady-state levels of eIF2α protein (13).
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bioRxiv - Microbiology 2021Quote: ... Proteins were blotted as previously described and detected using Anti-polyHistidine-Peroxidase monoclonal antibody (Sigma-Aldrich # A7058) and signals were revealed using the Metal Enhanced DAB Substrate Kit ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated by SDS-PAGE and probed with antibodies against zDHHC5 (1:1,000; Sigma Prestige, HPA014670), HA (1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins and DNA fragments were then immuno-precipitated using antibodies against anti-FLAG (SIGMA, catalog no. M2), H3Ac (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... and HA-tagged full length CASK proteins were incubated with 1μg anti-Flag M2 antibody (Sigma, F3165) at 4°C over night ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with an Anti-HA-Peroxidase antibody (Sigma-Aldrich, St. Louis, USA, 12013819001). For the RACB ubiquitination screening ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins underwent binding with secondary antibodies diluted 5000-fold in PBS (anti-rabbit IgG; Sigma-Aldrich). Protein bands were visualized by staining with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... Protein was detected using a secondary HRP-linked antibody and Luminata Classico Western HRP Substrate (Millipore WBLUC0020). To reprobe membranes ...
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bioRxiv - Plant Biology 2021Quote: ... Flag-tagged proteins were detected using the anti-Flag HRP-coupled antibody (mouse monoclonal, A8592; Sigma-Aldrich) diluted 1:5000 (v/v) ...
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bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were incubated overnight with 5 µL of Flag or V5 antibody-conjugated beads (Sigma-Aldrich) or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we employed antibodies against RNA-Binding Protein RBFOX3 (aka NeuN; mouse anti-NeuN, Alexa488-conjugated, MAB377X, Millipore) that is expressed in all neuronal nuclei ...
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bioRxiv - Microbiology 2021Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein G magnetic beads (TAMAGAWA SEIKI, TAS8848N1173) were pre-incubated with anti-FLAG antibody (SIGMA [M2], F1804) for more than 1 h at 4°C with gentle rotation before adding lysate ...
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bioRxiv - Developmental Biology 2024Quote: ... TBX33xFLAG proteins were detected by chemo-luminescence using monoclonal M2 anti-FLAG antibodies (1:500, Sigma, F1804) in combination with donkey anti-mouse IgG-HRP antibodies (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected by western blotting with antibodies mab2502 (clone 26) and mab1919 (clone 11C1.3) from Sigma.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Biochemistry 2023Quote: ... total protein was extracted and examined via western blots using HRP-conjugated anti-mouse secondary antibody (Sigma). Protein loading was visualized with Amido Black Staining Solution (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Monoclonal antibodies were purified from the cell culture supernatants by affinity chromatography on Protein G Sepharose (Sigma), using 0.1M glycine ...
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for detection of proteins by western blot: anti-M2-FLAG (Sigma, F3165), anti-β-tubulin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The expression of the recombinant proteins was checked by Western blotting with monoclonal anti-HA antibodies (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with anti-HA antibody and immobilized on protein A Sepharose beads (Sigma-Aldrich). Beads washed 3 times with lysis buffer and then washed twice with kinase buffer (1xPBS (pH7.4) ...