Labshake search
Citations for Millipore Sigma :
601 - 650 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: Antibodies against the following proteins were used at indicated dilutions: anti-acTub (Sigma-Aldrich T7451, mouse 1:2,000), anti-Arl13b (Proteintech 17711-1-AP ...
-
bioRxiv - Microbiology 2021Quote: ... The localisation of the codon-optimised NS5 protein was detected with a rabbit-anti-FLAG antibody (Sigma, Germany).
-
bioRxiv - Cancer Biology 2022Quote: ... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
-
bioRxiv - Molecular Biology 2023Quote: ... An equivalent amount of protein lysate was subjected to SDS-PAGE and immunoblotting with antibodies against FLAG (Sigma), V5 (Cell Signaling ...
-
bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
-
bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
-
bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
-
bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
-
bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
-
bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... the primary antibody was glial fibrillary acidic protein (GFAP) (1:1000 dilution; Millipore, Temecula, CA, USA; Cat. #MAB360). Secondary antibodies were conjugated to Alexa Fluor 594 (donkey anti-mouse ...
-
bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
-
bioRxiv - Neuroscience 2022Quote: ... for 2 hours and subsequently incubated with Dynabeads Protein G and an anti-Flag antibody (Sigma-Aldrich, F1804) at 4°C with rotation for 2 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... Western blotting was performed using a α-4HNE antibody specific to the Michael protein adduct (EMD Millipore Corp.) at a dilution of 1:1000 antibody over night at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... The presence of over-expressed protein was confirmed using monoclonal anti-poly histidine alkaline phosphatase antibody (Sigma-Aldrich) at 1:20,000 dilution.
-
bioRxiv - Biochemistry 2022Quote: ... The expression of the proteins was assessed by western blot using antibodies raised against the T7 epitope (Novagen) and GAPDH (Abcam).
-
bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... Changes in HCoV-OC43 spike protein expression were assessed using mouse monoclonal antibody 541-8F (Sigma-Aldrich, MAB9012). The effect of the compound on influenza A virus NS1 non-structural or nucleoprotein expression was measured using rabbit anti-influenza A virus NS1 nonstructural protein antibody (GeneTex ...
-
bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were assayed with a 2-min mix/2-min wait/5-min measurement cycle for three baseline measurements followed by three cycles after each injection of four compounds affecting bioenergetics: 0.5μM oligomycin (Complex V inhibitor; Sigma, St. Louis, MO, USA), 0.7μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP ...
-
bioRxiv - Molecular Biology 2023Quote: ... the slides were incubated for 30 minutes at room temperature with SABC (streptavidin and biotinylated horseradish peroxidase complex (SABC) reagent (Sigma-Aldrich, USA). The slides were then stained with the AEC agent kit (HiMedia Ltd ...
-
bioRxiv - Neuroscience 2023Quote: ... and immunoreactivity was visualized by the avidin-biotin complex method and sections were developed in diaminobenzidine (DAB, Sigma Aldrich, St. Louis, USA).
-
bioRxiv - Cell Biology 2023Quote: ... cholesterol was delivered by incubating cells for 1 h with 100 μM of water-soluble methyl-β-cyclodextrin–cholesterol complex (Sigma-Aldrich) in DMEM/F-12 ...
-
bioRxiv - Cell Biology 2024Quote: ... oocytes were isolated from the cumulus-oocyte complex by treating with 3 µg/ml hyaluronidase (H3506; Sigma-Aldrich, St Louis, MO, USA) for approximately 5 min.
-
bioRxiv - Genetics 2023Quote: ... The dilution ratios were as follows: 1:1000 for antibodies to kinetochore proteins and 1:100 for antibodies to α-tubulin (Sigma-Aldrich, St. Louis, MO; catalog number T6199). After washing twice for 5 minutes in 1× PBS at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
-
bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
-
bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
-
bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
-
bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
-
bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
-
bioRxiv - Physiology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes and immunoblot analyses were carried out using rabbit antibodies directed against ShcA (Millipore), Collagen X (Abcam) ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
-
bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
-
bioRxiv - Cell Biology 2021Quote: ... and the expression of the bait-protein was detected from the input sample with anti-FLAG antibody (Sigma Aldrich).
-
bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
-
bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
-
bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
-
bioRxiv - Developmental Biology 2020Quote: ... the supernatant was subjected to an immunoreaction with a specific antibody pre-conjugated with magnetic protein A beads (Millipore) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
-
bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
-
bioRxiv - Plant Biology 2020Quote: ... The expression of the target proteins was verified by western blot using ANTI-FLAG M2 Monoclonal Antibody (Sigma-Aldrich). ChIP assay was performed with ANTI-FLAG M2 Magnetic Beads (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Histone H3 lysine 27 trimethylated protein was detected using an anti-H3K27me3 antibody (rabbit polyclonal, Millipore, Burlington, MA, USA) diluted 1:500.
-
bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...