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Citations for Millipore Sigma :
5251 - 5300 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma contamination of cells was tested and ruled out with the LookOut Mycoplasma Detection kit (Sigma-Aldrich; catalog number: MP0035).
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bioRxiv - Bioengineering 2022Quote: ... The cell line was tested as being negative for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) and Jumpstart™ Taq DNA Polymerase (Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: ... The phosphate concentration was then measured according to the manufacturer’s instructions (Malachite Green Phosphate Assay Kit, Sigma-Aldrich, The Netherlands). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... growth and pattern of metastasis in vivo and routinely screened for Mycoplasma (LookOut® Mycoplasma PCR Detection kit, Sigma-Aldrich). None of the cells used are listed in the International Cell Line Authentication Committee (ICLAC ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were stained either for ALP activity using an ALP detection kit according to the manufacturer’s instructions (Sigma-Aldrich) or with crystal violet ...
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bioRxiv - Cell Biology 2023Quote: ... NPCs were differentiated to astrocytes using the Stem Cell Technologies STEMdiff astrocyte differentiation and maturation kits during which they were split weekly with Accutase (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Tissue was then blocked as per the manufacturers conditions before proceeding with the assay using the Duolink In situ proximity ligation assay PLA kit (Sigma). Antibodies used for PLA ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Ratios between the oxidized form NAD+ and the reduced form NADH were measured using NAD+/NADH quantification kit (MAK037, Sigma) with whole cell lysates of wild type S ...
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bioRxiv - Genomics 2023Quote: ... The material was ground with mortar and pestle and the RNA was extracted with the Spectrum Plant Total RNA kit (Sigma) and treated with DNAse I amplification grade (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... immunoprecipitated NS from the four S-phase fractions collected by flow cytometry or from an asynchronous cell population were amplified by whole-genome amplification (GenomePlex Complete Whole Genome Amplification kit #WGA2; Sigma) according to the manufacturer’s recommendations to obtain sufficient DNA amount ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Genomics 2022Quote: ... Genome extraction was then performed using the GenElute Bacterial Genomic DNA (gDNA) extraction kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the supernatant was collected and used for ATP assay using an adenosine 5-triphosphate (ATP) bioluminescent assay kit (Sigma-Aldrich). The generated bioluminescence signal was measured with a SpectraMax M5 plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Genomics 2023Quote: ... The bisulfite-treated DNA fragments were ligated with a nine-nucleotide adapter and amplified using a GenomePlex Single Cell Whole Genome Amplification Kit (Sigma) for three rounds ...
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bioRxiv - Neuroscience 2023Quote: ... all cultures were monthly tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma, cat. no MP0035-1KT).
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bioRxiv - Cancer Biology 2023Quote: RIP assay was performed following the manufacturer’s instructions (EZ-Magna RIP RNA-binding Protein Immunoprecipitation Kit, Sigma-Aldrich, #17-701). Antibody against eIF4G was used for the part of immunoprecipitation ...
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bioRxiv - Bioengineering 2023Quote: ... (Shrock, Shrock, and Elledge 2022) The ligated inserts were packaged with the T7Select packaging kit (Millipore Sigma, St. Louis, MO) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ER was purified according to the protocol optimised by combining the traditional microsome-based density gradient isolation method (Endoplasmic Reticulum Isolation Kit developed by Sigma) with the cell surface biotinylation reaction method (developed and optimised by Pierce) ...
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bioRxiv - Biochemistry 2023Quote: ... A standard cell-free reaction (2-3 ml of RM) was carried out in 50 ml tubes using a Pur-A-Lyzer Maxi dialysis kit (#PURX35050, Sigma). The TrkBtm was expressed as a precipitate ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Physiology 2023Quote: ... 1.5µl of haemolymph was used to measure the glucose and trehalose according to the Glucose assay kit (Sigma-Aldrich #GAGO20). To remove cells and tissue debris ...
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bioRxiv - Systems Biology 2023Quote: ... The amount of glucose remaining at each time point was measured by using a glucose-oxidase-peroxidase reaction kit (Sigma). Glucose phosphorylation activity was evaluated from the total amount of glucose consumed per unit of time.
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bioRxiv - Immunology 2023Quote: ... Neisserial genomic DNA was extracted from cell suspensions of overnight culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma). Genomic libraries were generated using Illumina Nextera DNA Flex Library Prep according to the manufacturer’s instructions and sequenced on Illumina MiSeq 2×250bp paired end platform ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of tGSH (nM/mg of erythrocyte pellet) was assayed using a commercial kit (Sigma-Aldrich, St Louis, MO) and according to (Galván and Alonso-Alvarez ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Glycerolipid content of male and female whole bodies and female ovaries and fat bodies was measured 12 days after dsGFP and dsXPO1 microinjection using the colorimetric Serum Triglyceride Determination kit (Sigma, Triglyceride Reagent T2449 ...
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Genomics 2023Quote: ... Cells were validated by STR analysis and regularly confirmed to be mycoplasma free by Venor GeM Mycoplasma Detection Kit (Milipore Sigma). 293T cells were cultured at 37 C ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Cancer Biology 2023Quote: The effect of Cul9 on GC cell proliferation was assessed by EdU assay using an In Vivo EdU Click kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed at 4 days in vitro (DIV4) with 4% PFA and the Duolink PLA kit was employed as previously described 7 following the manufacturer’s (Sigma-Aldrich) protocol 45.
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bioRxiv - Biochemistry 2023Quote: ... and determination of the released glycerol in 10 µl of the upper phase with a commercial Glycerol Assay Kit according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: The subtype of the mAb was IgG1 as determined using a subtype identification kit (Sigma-Aldrich, St. Louis, MO, USA). Non-immunoglobins in the ascites were removed by precipitation with caprylic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... and a random RT-PCR amplification was performed using the TransPlex® Complete Whole Transcriptom Amplification Kit WTA2 (Merck Millipore) following the manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... kidney and gonads where available and from adult testis and ovaries with the GenElute Mammalian Total RNA Miniprep Kit (Sigma) and DNase treated using DNA-free (Ambion ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 400 μg total RNA in 250 μl water was applied to a GeneElute mRNA miniprep kit (Sigma-Aldrich; MRN70-1KT), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...