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Citations for Millipore Sigma :
5401 - 5450 of 6010 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Neuroscience 2024Quote: ... Free and total cholesterol levels were determined in sciatic nerves methanolic extracts by cholesterol quantitation kit (MAK043–1KT–Sigma Aldrich) following manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Immunology 2024Quote: ... Infected erythrocytes were labeled with a membrane dye using the PKH26R Red Fluorescent Cell Linker Midi Kit (Sigma #MIDI26-1KT) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lipolysis was assessed from the release of FFA and glycerol in the secretion samples using non-esterified fatty acids measurement kit (FUJIFILM Wako Chemicals Europe GmbH) and free glycerol reagents (G7793 and F6428, Sigma) respectively and normalised to total protein contents.
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Genomics 2023Quote: ... Cells were validated by STR analysis and regularly confirmed to be mycoplasma free by Venor GeM Mycoplasma Detection Kit (Milipore Sigma). 293T cells were cultured at 37 C ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA used for ONT sequencing was poly(A+) selected in two consecutive rounds using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... After 24-hours BrdU was added to the media and 16h later cell proliferation was evaluated using the BrdU Cell Proliferation ELISA kit (EMD-Millipore). Colorimetric analysis was done with single channel 450nm intensity ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor α (TNFα)) was performed using the Milliplex Mouse Cytokine Magnetic Kit (EMD Millipore, Cat # MCYTOMAG-70K) and Luminex MAGPIX instrument at the Vanderbilt Hormone Assay and Analytical Service Core ...
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bioRxiv - Genomics 2023Quote: To obtain sufficient nuclei to run the same suspension across both fixed kits we used EZ lysis buffer (Sigma NUC101), at the expense of greater debris ...
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bioRxiv - Cell Biology 2023Quote: ... histone H1.2 knockout heterozygous (Het) and homozygous (KO) mice are isolated using the Nuclei Isolation ‘Kit Nuclei EZ Prep’ (NUC101, Sigma). Then the nuclei are treated with 500 µM MNNG with fresh-made NAD+ (N0632 ...
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bioRxiv - Immunology 2023Quote: The serum alanine aminotransferase (ALT) activity was analysed using a commercially available kit according to the manufacturer’s guidance (Sigma-Aldrich) and a Spark multimode microplate reader (Tecan ...
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bioRxiv - Immunology 2023Quote: ... the proteins of the parietal-cell cytosol and membrane/organelle fractions were extracted with the ProteoExtract Subcellular Proteome Extraction Kit (Millipore) to exclude the nucleic and cytoskeletal fractions ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA was extracted from three to four replicates per plant and stage using the Spectrum Plant Total RNA kit (SIGMA). MagNA Lyser Green Beads (Roche ...
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bioRxiv - Physiology 2023Quote: Free fatty acid (FFA) content was quantified in mouse plasma samples using the Free Fatty Acid Quantitation Kit (Sigma, #MAK0044) in line with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Plant Biology 2023Quote: ... bacteria were harvested by centrifugation and pellets were resuspended in 5 ml of binding buffer (His-bind buffer kit (Millipore), proteins were extracted using a Constant cell disrupter system (Constant Systems ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were quantified by isolating the genomic DNA from 1ml of inoculate using GenElute Bacterial Genomic DNA kit (Sigma) and measuring the genomic equivalents (GE ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed at 4 days in vitro (DIV4) with 4% PFA and the Duolink PLA kit was employed as previously described 7 following the manufacturer’s (Sigma-Aldrich) protocol 45.
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bioRxiv - Biochemistry 2023Quote: ... A standard cell-free reaction (2-3 ml of RM) was carried out in 50 ml tubes using a Pur-A-Lyzer Maxi dialysis kit (#PURX35050, Sigma). The TrkBtm was expressed as a precipitate ...
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bioRxiv - Cell Biology 2023Quote: ER was purified according to the protocol optimised by combining the traditional microsome-based density gradient isolation method (Endoplasmic Reticulum Isolation Kit developed by Sigma) with the cell surface biotinylation reaction method (developed and optimised by Pierce) ...
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bioRxiv - Biochemistry 2023Quote: Apoptotic cell death was detected using the The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7111, supplemental methods).
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma contamination of cells was tested and ruled out with the LookOut Mycoplasma Detection kit (Sigma-Aldrich; catalog number: MP0035).
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bioRxiv - Bioengineering 2022Quote: ... The cell line was tested as being negative for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) and Jumpstart™ Taq DNA Polymerase (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... growth and pattern of metastasis in vivo and routinely screened for Mycoplasma (LookOut® Mycoplasma PCR Detection kit, Sigma-Aldrich). None of the cells used are listed in the International Cell Line Authentication Committee (ICLAC ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were stained either for ALP activity using an ALP detection kit according to the manufacturer’s instructions (Sigma-Aldrich) or with crystal violet ...
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bioRxiv - Cell Biology 2023Quote: ... NPCs were differentiated to astrocytes using the Stem Cell Technologies STEMdiff astrocyte differentiation and maturation kits during which they were split weekly with Accutase (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Ratios between the oxidized form NAD+ and the reduced form NADH were measured using NAD+/NADH quantification kit (MAK037, Sigma) with whole cell lysates of wild type S ...
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bioRxiv - Genomics 2023Quote: ... The material was ground with mortar and pestle and the RNA was extracted with the Spectrum Plant Total RNA kit (Sigma) and treated with DNAse I amplification grade (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... immunoprecipitated NS from the four S-phase fractions collected by flow cytometry or from an asynchronous cell population were amplified by whole-genome amplification (GenomePlex Complete Whole Genome Amplification kit #WGA2; Sigma) according to the manufacturer’s recommendations to obtain sufficient DNA amount ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Genomics 2022Quote: ... Genome extraction was then performed using the GenElute Bacterial Genomic DNA (gDNA) extraction kit (Sigma-Aldrich, St. Louis, MO, USA) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted using GenEluteTM Mammalian Total RNA Miniprep Kit and On-Column DNase I Digestion Set (both Sigma-Aldrich) and a total of 9 samples fulfilling the submission requirements (0.5-1.0μg total RNA in nuclease free dH2O with A260/A280 and A60/A230 ratios above 1.8 ...
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bioRxiv - Genomics 2023Quote: ... The bisulfite-treated DNA fragments were ligated with a nine-nucleotide adapter and amplified using a GenomePlex Single Cell Whole Genome Amplification Kit (Sigma) for three rounds ...
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bioRxiv - Bioengineering 2023Quote: ... (Shrock, Shrock, and Elledge 2022) The ligated inserts were packaged with the T7Select packaging kit (Millipore Sigma, St. Louis, MO) as per manufacturer’s instructions ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Synthetic Biology 2024Quote: ... run on a 2.5 % (w/v) agarose gel at 100 V for 2 h and purified using a gel extraction kit (Sigma-Aldrich). The products were then used as templates for a second PCR with distinct combinations of barcoded primers (Supplementary Table 10 ...
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bioRxiv - Molecular Biology 2023Quote: Glycerolipid content of male and female whole bodies and female ovaries and fat bodies was measured 12 days after dsGFP and dsXPO1 microinjection using the colorimetric Serum Triglyceride Determination kit (Sigma, Triglyceride Reagent T2449 ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...