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Citations for Millipore Sigma :
5251 - 5300 of 6010 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of immunoprecipitate (IP) to Input was calculated as described in the Magna MeRIPTM m6A kit (17-10499, Millipore). mRNA controls (m6A RNA and unmodified RNA ...
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bioRxiv - Genetics 2019Quote: Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Rhodamine In situ Apoptosis Detection Kit; Millipore). Embryos were fixed ...
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bioRxiv - Pathology 2019Quote: ... treated with opsonized RBCs (1:10) and intracellular ROS was determined with a fluorometric intracellular ROS kit (Sigma-Aldrich, MAK142). Stained cells were visualized and captured using Leica DMI6000B with Cy5 filter set ...
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bioRxiv - Genetics 2021Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
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bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... Enrichment of the chloroplast from the leaf samples was performed using ± 2 cm2 of leaf tissue with the Chloroplast Isolation Kit (Sigma, CPISO-1KT After this the DNA was extracted using a modified C-Tab protocol (Doyle JJ ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Insulin and glucose were measured in plasma samples using the Sensitive Insulin enzyme immunoassay kit (EZRMI-13K, Millipore, Billerica, MA) and Glucose GOD-PAP enzyme immunoassay kit (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted as per manufacturer instruction and genomic DNA was removed using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Bioengineering 2021Quote: ... the pellets were collected to examine the cell lysis interference using a Glucose-6-Phosphate Dehydrogenase kit (Sigma-Aldrich, USA). The details of EPS extraction and analytical methods for various EPS components (proteins ...
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bioRxiv - Cell Biology 2021Quote: ... G6P concentrations were determined at 2 hrs and 6 hrs post activation and compared to unactivated controls using a G6P kit (Sigma) and following manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... 5% CO2 tissue culture incubators and routinely confirmed to be mycoplasma negative using LookOut mycoplasma PCR detection kit (Sigma Aldrich). pLifeAct-mScarlet-N1 and pLck-mVenus-C1 were gifts from Dorus Gadella (Addgene plasmids #85054 and #84337 ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Cell Biology 2021Quote: ... The PKH26:MYH10-KD co-cultures 3T3-L1 cells were labeled with 0.5λ PKH26 Fluorescent Cell Linker Kit (Sigma-Aldrich); the GFP cells were added after one day.
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were washed once in PBS and nuclei obtained using the EZ Prep nuclei kit then incubated with 50μM Azide-PEG3-biotin conjugate (Sigma-Aldrich) was dissolved in prewarmed Buffer A and added to samples. ...
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bioRxiv - Biochemistry 2020Quote: ... The cells and media were separated after the incubation and processed for quantification of insulin levels using the RI-13K Rat Insulin RIA Kit (Millipore) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitates were applied to 8 % SDS-PAGE and the gel was stained by ProteoSilver™ stain kit (Sigma-Aldrich) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from the in vitro samples using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, MO, USA) according to the manufacturers’ instructions ...
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bioRxiv - Genomics 2021Quote: The high-quality genomic DNA was isolated from all three strains using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression vectors for human Arf1 (UniProtKB: P84077) and Arf6 (UniProtKB: P62330) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described for protein expression of human Rab-family small GTPases (Tamura and Mima ...
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bioRxiv - Plant Biology 2021Quote: The coding sequences of most of the 44 non-pseudogene AtUMAMIT were amplified by PCR (oligonucleotide sequence is provided in Supplemental Table 1) by using the KOD Hot Start DNA Polymerase kit (Novagen) from a mix of reverse-transcribed RNA extracted from seedlings and inflorescences ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated at different time points using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). Aliquots with 50 ng RNA were subjected to DNAse treatment (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... PCR-positive colonies were grown and the vector was extracted using GenElute Plasmid Miniprep Kit following manufacturer’s protocol (Sigma-Aldrich). The vector was then transformed into expression strain E ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... GP:NPC1 interaction was detected by applying the Duolink® In Situ Detection Reagents Red kit following the manufacturer’s instructions (Sigma). After removing excess reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting plasma supernatant was transferred to a sterile Eppendorf tube and stored at −80 °C until the cytokines were measured using a Milliplex xMAP Mouse High Sensitivity TCell Magnetic Bead Panel kit MHSTCMAG-70K (Millipore) following the kit protocol on a Luminex 200 system with Xponent (v2.7) ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole genome amplification was performed using SeqPlex™ Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... pre-sorted or sorted cells were stained with either trypan blue or a Calbiochem live/dead double staining kit (Millepore Sigma) which uses a cell permeable green fluorescent Cyto-dye to stain live cells and propidium iodine to stain dead cells ...
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bioRxiv - Cancer Biology 2022Quote: Oleoyl-L-α-lysophosphatidic acid (LPA, L7260) and a Periodic Acid-Schiff (PAS) Kit (395B) were purchased from Sigma-Aldrich. The PKD inhibitor CRT0066101 (A8679 ...
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bioRxiv - Plant Biology 2022Quote: ... total RNA was extracted from 14 day-old Arabidopsis thaliana seedlings using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) and treated with DNAase I (RapidOut DNA Removal Kit ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana seedlings for each line grown on a ½ MS agar plate using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... resuspended in RNA extract lysis buffer (100µL RLT lysis buffer from RNA extraction kit supplemented with 40mM DTT, Sigma, #D9779), vortexed and let sit (10min. ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Neuroscience 2022Quote: The MILLIPLEX® MAP human amyloid beta and tau magnetic bead panel 4-plex ELISA kit (Millipore, Burlington, MA, USA) and the Bio-Plex 200 System instrument (Biorad ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# AS1107) for one hour at room temperature and visualized using the Immobilon ECL substrate kit (Millipore, Merck KgaA, Germany). Primary antibodies specific to PLLP (1:700 ...
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bioRxiv - Genetics 2022Quote: ... Cell death analysis was performed using the ApopTag® Fluorescein in situ Apoptosis Detection Kit (Sigma-Aldrich, Cat. No. S7110) following manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... obtained by grinding in liquid nitrogen and using a SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, DE) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiac tissues were fractionated into nucleus-enriched and cytoplasmic samples by using a CelLytic™ NuCLEAR Extraction kit (Sigma, Nxtract), as previously described (32).
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bioRxiv - Genetics 2022Quote: ... To assess editing efficiency genomic DNA was isolated from CRISPR-edited and control cells using a GenElute Mammalian DNA Miniprep Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50) and l μl of eluted RNA was used for complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... the PCR reaction described above was repeated using genomic DNA isolated using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... histolytica trophozoites were grown with and without queuine (0.1μM for three days) and total RNA was isolated using the TRI reagent kit according to the manufacturer’s instructions (Sigma-Aldrich). Three biological replicates from each treatment condition were processed ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...