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Citations for Millipore Sigma :
5651 - 5700 of 6010 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... ∼50 mg of tissue was ground under liquid nitrogen and the total RNA was ex-tracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) following the manufac-turer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: The cell toxicity of the drugs was determined using Cell Counting Kit-8 (CCK-8) (Catalog No.: 96992-100TESTS-F, Sigma-Aldrich) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal deletion mutants were verified by amplification of the genomic region from isolated genomic DNA (GeneElute Bacterial Genomic DNA Kit, Sigma Aldrich) and by whole genome sequencing (MicrobesNG/Plasmidsauraus) ...
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Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging responsebioRxiv - Plant Biology 2023Quote: ... The tissue was ground in liquid nitrogen and total RNA was extracted using Spectrum™ Plant Total RNA kit (Sigma-Aldrich). For each condition (waterlogged or untreated) ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA from mouse liver or striatum (see below) was extracted using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma #G1N350).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Alkaline-phosphatase (Alk-Phos) positive cells were assessed on day 18 in culture using Leukocyte Alkaline Phosphatase Kit (Sigma; cat#86R) following manufacturer instructions.
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bioRxiv - Cell Biology 2023Quote: ... RPE cells were harvested from WT and Akt2 KI mice and ChIP was performed using a commercially available kit (17-295, EMD Millipore). The following primers for the Atg9b promoter and negative control were used Atg9B_CHIP_F1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was then performed using 200 ng of 4C-template DNA with the Expand Long Range PCR kit (Millipore, 4829042001) under specific PCR conditions (94 °C for 2 min ...
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bioRxiv - Microbiology 2023Quote: PLA: PLA was carried out using the Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich, Cat #DUO92101-1KT). Briefly ...
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bioRxiv - Physiology 2023Quote: ... the supernatant and the fasting and 15 min GTT serum was performed using Rat/Mouse Insulin ELISA kit (EZRMI-13K, Sigma-Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit and the On-Column DNase I Digestion Set (both from Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Total genomic DNA from cecal digesta was extracted using PowerSoil® DNA Isolation Kit (Sigma-Aldrich, Carlsbad, United States of America). DNA concentration and purity were assessed using a Synergy HTX (Gene Company Limited ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... For signal amplification the ABC Kit (Vector, Burlingame, CA, USA) was used before applying 3,30-diaminobenzamidine (Sigma-Aldrich, St Louis, USA) as a chromogen ...
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bioRxiv - Microbiology 2023Quote: ... the resulting powders were employed for total RNA extraction with “Spectrum Plant Total RNA” kit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s protocol (20).
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bioRxiv - Microbiology 2023Quote: ... the reactions were heated to 90 °C for 10 minutes and then allowed to gradually cool to room temperature and RNA was purified using the GenElute Mammalian Total RNA kit (Sigma RTN350) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with 1× PBS and i ncubated with anti-mouse and anti-rabbit plus PLA probes (PLA kit, Sigma-Aldrich) at 37 °C for 1 h ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from plant tissue samples using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St Louis, MO, USA) followed by DNase digestion with RNase free DNase Set (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: C6 cells were treated with indicated nanoformulations for fixed time points followed by Annexin V/ propidium iodide (PI) staining using Annexin V-FITC Apoptosis Detection Kit II (Cat # PF032, Sigma-Aldrich), as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Migrated cells on the bottom side of the membrane were either stained with crystal violet or were collected by trypsination and counted using a cell titer blue staining kit (CTB; Sigma-Aldrich). For measuring invasion ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to PLA with an anti-Rad51 and anti-VE-Cadherin (see Supplementary Table 1) using the Duolink in situ red starter kit (mouse/rabbit) (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using the PEG/Lithium acetate method (Yeast Transformation Kit, MilliporeSigma)(Gietz and Woods 2002) and plated on non-inducing selective synthetic media (Sigma-Aldrich® Yeast Synthetic Drop-out Media Supplements ...
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bioRxiv - Genetics 2024Quote: ... digestion at 37 ᵒC for 20 minutes and used directly for bisulfite treatment using the Imprint DNA Modification Kit (Sigma MOD50) according to the manufacturer’s 2-step modification procedure ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... the gradient-purified virus was subjected to buffer exchange using the Pur-A-Lyzer™ Maxi Dialysis Kit (20k MWCO) (Sigma) at 4°C overnight to replace the iodixanol with PBS.
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bioRxiv - Bioengineering 2023Quote: ... All the cell lines were regularly tested for mycoplasma contamination using the LookOut Mycoplasma PCR detection kit (Product no. #MP0035 Sigma-Aldrich) and maintained in humidified cell culture incubators at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... A fraction of the cell lysates was used to estimate NAD(H) levels using the NAD/NADH quantification kit (Sigma MAK037) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: The segregation analysis was performed on genomic DNA extracts of Synechocystis and Synechocystis mutants obtained using the Gen-Elute Bacterial Genomic DNA Kit (Sigma-Aldrich) by effectuating a screen for GGR and ShMKS incorporation into slr0168 using PCR ...
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bioRxiv - Neuroscience 2023Quote: Nuclei were extracted from mouse frontal cortical tissue using the Nuclei PURE Prep kit (NUC201-1KT, Millipore Sigma, Burlington MA, USA) with volumes reduced to a quarter of the recommended amount ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Microbiology 2023Quote: A fresh culture of ULC029 in BG11 was used to extract genomic DNA using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) following the recommendations of the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Bioengineering 2023Quote: ... This cell inoculated hydrogel precursor was injected into central tissue lane of MFOC devices and incubated for 30 minutes prior to filling the side channels and media reservoirs with endothelial cell growth medium (VEGF kit) containing 25 µg/mL aprotinin (Sigma-Aldrich) to inhibit fibrin degradation.
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from different vegetative and reproductive developmental stages using SpectrumTM Plant Total RNA isolation kit according to manufacturer’s instruction (Sigma-Aldrich, USA). The quality of isolated total RNA was determined in a Nanodrop (ND-1000 ...
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bioRxiv - Microbiology 2024Quote: ... The absence of mycoplasma contamination in the virus stocks was confirmed using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma; #MP0035).
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bioRxiv - Cell Biology 2024Quote: ... was performed to detect the interactions between ITGβ1 and CD44 using Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... immune cell samples were first heat inactivated at 70°C for 10 mins and then subjected to metabolite analysis using GOD-POD kit (Sigma#GAGO20) and Triglyceride assay kit (Sigma#T2449 ...
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bioRxiv - Bioengineering 2024Quote: ... Unbound dye was removed using dialysis against a 1000-fold excess of PBS or Broccoli aptamer buffer at 4 °C overnight with the Pur-A-LyzerTM Mini 6000 Dialysis Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Recovered adherent cells were resuspended in MSC medium after centrifugation and counted using the GuavaEasycyte 8HT apparatus in Muse Count & viability kit (MCH600103, Cytek Biosciences) or with trypan blue (T8154, Sigma-Aldrich). Part of cells were frozen at 1×106 cells/vial in FBS containing 10% DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... the supernatant was diluted and subjected to H3K9me3 ChIP using the ChIPAb+ Trimethyl-Histone H3 (Lys9) kit (Millipore, cat# 17-10242). Bound material was recovered after incubation with 15 μL of protein G beads (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed in tNCC or SHEPMYCN cells after 24 h Dox induced overexpression of MYCN using a Duolink PLA kit (Sigma, DUO92014) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Neuroscience 2024Quote: ... digoxygenin (DIG)-labeled antisense probes were prepared using the Roche DIG labeling kit (Sigma Cat # 11277073910 and T7 (Sigma Cat # 10881767001) or T3 (Sigma Cat # 11031163001 ...
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bioRxiv - Neuroscience 2024Quote: ... “Indirect” PLAs for nitrated hASYN and nitrated NDUFB8 were performed using probes already conjugation to secondary antibodies and provided in a PLA kit (Duolink, Sigma-Aldrich). Primary antibodies used for indirect PLA were ...
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bioRxiv - Bioengineering 2024Quote: ... the expression of early differentiation marker (alkaline phosphatase, ALP) and late-stage differentiation marker (calcium mineralization) were quantified using ALP staining kit (Sigma Aldrich) and Alizarin Red S (ARS ...
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bioRxiv - Cell Biology 2024Quote: ... β-galactosidase (X-gal) lung staining was performed on frozen lung sections using the Senescence Cells Histochemical Staining Kit (Sigma CS0030) following manufacturer instructions ...