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Citations for Millipore Sigma :
5551 - 5600 of 6010 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Pf2-HA_OE and pf2-HA_KO ChIP and input control samples) was recovered from Proteinase K treated samples using the GenElute PCR purification kit (Sigma-Aldrich).
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bioRxiv - Genomics 2022Quote: ... The next day the DNA was extracted from 500 µl of culture using the GeneElute bacterial genomic DNA kit (Sigma-Aldrich). gDNA was quantified ...
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Genomics 2019Quote: Aphid samples were ground to a fine powder and total RNA was extracted using a plant RNA extraction kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was isolated from expanded clones after the second FACS sorting step using the GenElute mammalian genomic DNA miniprep kit (Sigma-Aldrich) and amplified in a PCR reaction using primers P1/P5 (Table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was kept for proteomics analysis and electrophoresis followed by silver staining after addition of fresh 6x sample buffer (ProteoSilver Silver Stain Kit, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica, Massachusetts, USA). Huh7.5-SGR cells were seeded in Petri dishes at 106 cells/dish for 24 h prior to transfection with pKSRP-HA plasmids ...
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bioRxiv - Developmental Biology 2019Quote: ... H9 hESCs were nucleofected using the Lonza P3 Primary Cell 4D-Nucleofector X Kit and monoclonal colonies were selected for 7-10 days with 1 μg/ml of puromycin (Sigma-Aldrich). Tetracycline hydrochloride (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: RNA isolation was carried out with 2 g of soil using RNA PowerSoil Total RNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA. USA) with phenol chloroform isoamyl alcohol 25:24:1 (Sigma-Aldrich) following the instructions of the manufacturer and resulting in 100 μL of RNA solution ...
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bioRxiv - Genomics 2019Quote: ... with 2 mL of ice-cold EZ-Prep lysis buffer from the Nuclei EZ-prep Isolation Kit (Sigma, Cat #NUC-101). The tissue was dounced 25 times with Pestle A ...
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bioRxiv - Cell Biology 2019Quote: ... the ligation experiment was performed according to the manufacturer’s instructions using the Duolink® In Situ Orange Starter Kit Goat/Rabbit (Sigma-Aldrich). Briefly ...
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bioRxiv - Physiology 2019Quote: ... TG content of cells before and at the end of adipogenic differentiation was performed as previously described (Dirat et al, 2011) using commercial kit (Sigma-F6428).
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The template for the bulk DNA probe was generated using isolated nuclei (Galbraith et al., 2011) and GenElute™ Plant Genomic DNA Miniprep Kit (Sigma).
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bioRxiv - Genomics 2019Quote: ... Individual colonies were cultured overnight before isolation of plasmid DNA using the GenElute™ miniprep kit (Sigma-Aldrich, Catalogue No. PLN350). Purified plasmids were Sanger sequenced to confirm successful cloning ...
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bioRxiv - Microbiology 2019Quote: ... These clones were grown separately overnight in lysogeny broth (LB) medium and used for plasmid extraction with GenElute Plasmid Miniprep Kit (Sigma-Aldrich). PCR amplifications ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA was isolated from the si-NC and si-Dnmt3aos-transfected M(IL-4) macrophage cells and was then treated with bisulfite using the Imprint DNA Modification Kit (Sigma-Aldrich). Bisulfite sequencing and pyrosequencing were conducted by Shanghai Sangon Biotech Corporation ...
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bioRxiv - Genetics 2020Quote: ... the immune peroxidase was detected using a Vectastain ELITE ABC kit (Vector) and visualized with a solution of diaminobenzidine (Sigma Aldrich) in the presence of 0.01% H2O2 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... then stained with 1% Crystal Violet in 2% ethanol for 20 min for NIH3T3 cells and MCC cells were counted using a Cell Counting Kit-8 (CCK-8) (Sigma-Aldrich). The stained cells on the lower side were counted under a microscope from 5 different randomly selected views ...
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bioRxiv - Plant Biology 2021Quote: Preparation of nuclear and cytoplasmic fractions was performed according to the user manual supplied with the CelLytic™ PN Plant Nuclei Isolation/Extraction Kit (Sigma) with minor modifications ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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bioRxiv - Immunology 2020Quote: ... post-fixed with 1% osmium tetroxide and mordanted with 1% tannic acid followed by dehydration through an ethanol series (contrasting with uranyl acetate at the 30% stage) and embedding with an Epoxy Resin Kit (all from Sigma-Aldrich). Ultrathin sections cut on a Leica UC6 ultramicrotome were contrasted with uranyl acetate and lead nitrate ...
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bioRxiv - Plant Biology 2019Quote: ... DNA was extracted from wild type Ps LMG5084 and its PL1-insensitive mutants using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). Libraries were prepared with the NEBNext Ultra II library kit according to the manufacturer’s instructions and sequenced on the Illumina HiSeq X platform to obtain 150 bp paired end reads with an average depth of 40-fold ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: M6A enrichment followed by qRT-PCR was used to quantify the changes in m6A methylation of the target gene using the Magna MeRIP m6A Kit (Millipore, MA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Tissue powder was then resuspended in a lysis buffer from the Mammalian Cell Lysis kit containing a protease inhibitor cocktail (Sigma-Aldrich). Samples were incubated in lysis buffer at 4°C for 15 min rotating and then centrifuged for 20,000 x g for 10 min at 4°C to pellet cell debris ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Physiology 2021Quote: ... extra blood was collected at each time-point and later plasma was separated by centrifugation (15min at 2,000 RPM) and stored at −80 °C for insulin measurement using a Rat/Mouse Insulin Elisa kit (EMD Millipore - EZRMI13K). Prior to sacrifice ...
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bioRxiv - Immunology 2020Quote: ... IL-17A and TNFα were quantified in culture supernatants using the mouse-specific Milliplex® multi-analyte panel kit MT17MAG-47K (Millipore; Sigma) and the MagPix® instrument platform with related xPONENT® software (Luminex Corporation) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... Both the antibodies targeted against Imp-β1 and puromycin were conjugated directly to the PLA probes as PLAplus and PLAminus respectively using Duolink™ In Situ Probemaker PLUS and MINUS kits (Sigma). Neurons were then fixed with 3.2 % PFA before processing with immunocytochemistry.
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as previously described (41,44) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as described previously (Rumi et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Microbiology 2020Quote: ... Peroxidase-conjugated secondary antibodies (Antgene, 1: 5000) were applied accordingly followed by image development with Chemiluminescent HRP Substrate Kit (Millipore Corporation).
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bioRxiv - Microbiology 2020Quote: Nuclear extracts (using NE-PER™ nuclear Extraction Kit (Thermo Fischer Scientific, USA) and whole-cell lysates (using RIPA buffer, Sigma) were prepared from MTB-infected macrophages (MOI 20:1) ...
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bioRxiv - Microbiology 2019Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Immunology 2021Quote: ... post-fixed with 1% osmium tetroxide and mordanted with 1% tannic acid followed by dehydration through an ethanol series (contrasting with uranyl acetate at the 30% stage) and embedding with an Epoxy Resin Kit (all from Sigma-Aldrich). Semi-thin 0.5 μm sections were cut and collected onto clean glass slides and dried at 60°C before staining with 1% Toluidine Blue and 1% Borax (all from Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and cloned into the mid-copy T7-FNS2 phage display vector as described previously,17 and packaged using the T7 Select Packaging Kit (EMD Millipore). Library quality assessment was performed as described in Results.
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Treatment history shapes the evolution of complex carbapenem-resistant phenotypes in Klebsiella spp.bioRxiv - Microbiology 2022Quote: Genomic DNA of the Klebsiella isolates was prepared from solid media scrapings of pure culture using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) and the Gram-negative bacteria protocol ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin sections were assayed for apoptotic cells using the ApopTag Plus Fluorescein in Situ Apoptosis Detection Kit (Millipore, Cat. #S7111) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 100-150 mg of snap-frozen tissue were mechanically homogenized in 750 µL of Lysis Solution/2-mercaptoethanol mixture and total RNA was isolated using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). Reverse transcription for up to 2 µg of total RNA was performed using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines including stable clones were routinely evaluated for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were analyzed on a 1.2 % agarose-gel and extracted with Kit Montage Gel Extraction columns (Millipore, Bedford, MA). The purified amplicons were used in a BP-reaction for the creation of Gateway-Entry vectors using 1 μL purified amplicon ...