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Citations for Millipore Sigma :
5201 - 5250 of 6010 citations for Resistant Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... TUNEL staining was performed in a set of sections using the ApopTag in situ apoptosis detection kit (S7165, Millipore). Sections were visualized under confocal microscopy (Nikon) ...
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bioRxiv - Neuroscience 2024Quote: ... A fluorometric intracellular ROS detection kit was used to detect ROS levels at 30 and 120 minutes (Sigma, MAK144) using a Varioskan microplate reader (ThermoFisher).
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from a small piece of Arabidopsis leaf using Extract-N-Amp extraction kit (Sigma-Aldrich) and used as a template for PCR ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: Nuclei from 10-15 million AMO1 cells were isolated using the Nuclei EZ Prep Nuclei Isolation kit (Sigma Aldrich), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from the tissues of 3DPG seedlings using the Spectrum Plant RNA Isolation Kit (Sigma-Aldrich). The RNA was treated with RNase-free RQ1 DNase (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was isolated from stressed and control plants (three biological replicates each) using SpectrumTM Plant Total RNA Kit (Sigma). Quality control of all the extracted RNA (triplicates for each condition ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA extraction from the thalli was carried out using the Spectrum Plant Total RNA kit from Sigma Aldrich. The quality and concentration of the extracted RNA samples were assessed using a Bioanalyzer (Agilent 2100) ...
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bioRxiv - Developmental Biology 2024Quote: ... The total isolated mRNA was reverse transcribed using First Strand cDNA Synthesis Kit for RT-PCR (Sigma Aldrich 11483188001). RT-PCRs were conducted using Taq polymerase (Invitrogen EP0401) ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA extracted from parasite cultures was regularly tested for Mycoplasma contamination (look out Mycoplasm PCR detection kit (Sigma)) using MSP primers 44 All transgenic P ...
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bioRxiv - Microbiology 2024Quote: Cell viability was assessed using the lactate dehydrogenase (LDH) enzyme activity method (Cytotoxicity Detection Kit version 11; Sigma-Aldrich) to quantify compromised cells in response to exposure to antibiotics ...
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bioRxiv - Bioengineering 2024Quote: ... the cells remaining in the microtube were labeled using the PKH26 lipophilic dye kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Neuroscience 2024Quote: Nuclei were isolated from 10-μm fresh-frozen sections using the Nuclei PURE Prep Nuclei Isolation Kit (Sigma-Aldrich), with specific modifications ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from cotton leaves using a Spectrum Plant Total RNA Kit (Sigma-Aldrich, Burlington, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the Foxp3/Transcription Factor Staining Kit (eBioscience) was used accordingly with conjugated antibodies against Olig2 (clone 211F1.1; Sigma Millipore). An Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the Foxp3/Transcription Factor Staining Kit (eBioscience) was used accordingly with conjugated antibodies against Olig2 (clone 211F1.1; Sigma Millipore). An Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
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bioRxiv - Bioengineering 2024Quote: The cell proliferation and viability after BNPs incubation was evaluated using cell counting kit-8 (cck-8, Sigma Aldrich), following the manufacturers’ instructions ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once with PBS and RNA extraction was performed using GenElute Mammalian Total RNA Miniprep Kit (Sigma), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1 x 106 stable cells using the GenElute mammalian genomic DNA kit (G1N350, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Serum concentrations of Metabolic Panel (Ghrelin, Glucagon, Insulin and Leptin) were determined using the Mouse Metabolic Magnetic Bead Panel Milliplex MAP kit (Millipore).
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bioRxiv - Microbiology 2021Quote: ... IgE) and IgA in feces supernatant were determined using the Mouse Immunoglobulin Isotyping Magnetic Bead Panel Milliplex MAP kit (Millipore) and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... This was then followed by genomic DNA removal and cleaning using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2021Quote: Tail tip fibroblasts were seeded in 96-well plates and the mitochondrial membrane potential (MMP) was determined using the mitochondrial membrane potential kit (Sigma) following the recommendations made by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Cell Biology 2020Quote: ... BAC clone (RP11-293M10) (CHORI) was fluorescently labeled using Cy3-dUTP (ENZO) and nick translation kit according to the manufacturer’s instructions (Sigma-Aldrich). Human Cot-1 (DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... After 9 days 3-5 × 106 cells from selection conditions and 100 × 106 non-selection conditions were washed 3 times with PBS and pelleted by centrifugation for genomic DNA extraction using GeneElute Mammalian Genomic DNA Miniprep kit (Sigma) and Quick-DNA Midiprep Plus kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Genomics 2020Quote: Between 250 ng and 2 µg of gDNA extracted from each tissue sample was bisulfite treated with an Imprint DNA Modification Kit (Sigma). Control samples lacking DNA template were run to ensure that there was no contamination during the bisulfite conversion of DNA ...
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bioRxiv - Genetics 2021Quote: ... were ground to a powder under liquid nitrogen and RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Bisulphite conversions were carried out using the two-step modification procedure of the Imprint® DNA Modification Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ChIP assay was used to detect the enrichment of HDAC3 in the promoter region of the miR-17-92 cluster and that of p65 in the Pgf promoter region using the EZ-Magna ChIP TMA kit (Millipore). The lung fibroblasts of each group in logarithmic growth phase were cross-linked and cultured with 1% formaldehyde for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... E11.5 embryos were dissected into ice-cold PBS and associated yolk sacs used for rapid genotyping using the Extract-N-Amp Tissue PCR kit as recommended by the manufacturer (Sigma). During genotyping ...
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bioRxiv - Developmental Biology 2021Quote: ... The medium was changed every two days for seven days before detection of alkaline phosphatase-positive colonies (Alkaline Phosphatase detection kit, Merck-Millipore). Alkaline phosphatase-positive colonies were analyzed and quantified using ImageJ analysis software (81).
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was extracted from single blastocysts by placing each blastocyst in a microtube containing 4.4 µl Extraction buffer (REDExtract-N-Amp Tissue PCR Kit, Millipore Sigma) mixed with 1.1 µl of Tissue Prep Buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The homogenate was heat inactivated at 70 °C for 5 min and then centrifuged at 14000 rpm for 3 min. Serum triglyceride determination kit (Cat. # TR0100) from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted from 5 individual embryos at mid-gastrula (st. 11) or later employing the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). The genomic region containing the CRISPR / Cas9 targeted region was PCR amplified using a nested PCR approach (Table 1 ...
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bioRxiv - Neuroscience 2020Quote: The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; #D5025, Sigma). OPCs were purified by immnopanning approach according to our previous protocol (Lang et al. ...
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bioRxiv - Molecular Biology 2021Quote: PLA was performed on cultured granulosa cells using the Duolink PLA Probes and PLA Fluorescence in situ Detection Kit Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: T7-Pep2,32 a complete human proteome library was restriction cloned into the T7-SEPARATE vector using the EcoRI/XhoI sites.32,33 The library was packaged in vitro using the T7Select Packaging Kit (Millipore Sigma) and expanded by plate lysate amplification to obtain an average clonal representation of ~100 plaques per peptide ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
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bioRxiv - Molecular Biology 2019Quote: ... Black Gold staining was conducted according to the manufacturer’s instructions (Black Gold II myelin staining kit, EMD Millipore, Billerica, MA). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50), treated with DNaseI on columns (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: ... Hybridization was performed at 50°C O/N with the probe 1.6 kb fragment containing the sequence (TTAGGG)n63 labelled with Nick Translation Kit (Sigma-Aldrich) and developed according to62 ...