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Citations for Millipore Sigma :
5101 - 5150 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Developmental Biology 2023Quote: Cells were lysed in membrane buffer (150 mM NaCl, 50 mM Tris-HCl pH 7,5, 1 mM 2-mercaptoethanol (Sigma, #M3148) and 1% CHAPS (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The cell drop was then immersed in 2 mL Leibovitz L15 -/-medium containing 1 μL/mL Hoechst dye (Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Genomics 2023Quote: ... 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393], 1% [w/v] sodium deoxycholate [Sigma, D6750] ...
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bioRxiv - Genomics 2023Quote: ... 40 mM KCl, 20 mM MgCl2, 2% [v/v] polyoxyethylene [10] tridecyl ether [Sigma, P2393], 1% [w/v] sodium deoxycholate [Sigma, D6750] ...
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bioRxiv - Cancer Biology 2024Quote: ... xenograft tumors excised from mice were weighed before immediate mechanical and enzymatic dissociation in DMEM-collagenase media for 1 hour in a 37°C shaker at 150 rpm (Hyclone DMEM, 2 mg/mL Collagenase type IV (Sigma), 5% FBS (Atlas Biologicals) ...
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bioRxiv - Pathology 2024Quote: ... The nematodes (100 worms/100 μl) were treated for 2 h in 1.5 ml tubes with 0-1 mM ascorbic acid (Sigma-Aldrich), 0-200 μM doxycycline hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... The infectious bloodmeal consisted of a 2:1 mix of washed rabbit erythrocytes (BCL) supplemented with 10 mM adenosine triphosphate (Sigma) and viral suspension supplemented with Leibovitz’s L-15 medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid, ABMBA, #R435902, Sigma) and centrifuge-filtered (0.22 µm hydrophilic PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in PBS with 2% HI-FBS (Hyclone, #SH30910.03) containing 1 µg/mL DAPI (Sigma Aldrich, #D9542) to detect dead cells ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor nodules from each mouse were diced with scissors then incubated for one hour at 37 °C in 2 mL of 1 mg/mL collagenase type IV (Sigma) in RPMI media ...
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bioRxiv - Immunology 2024Quote: ... coli at MOI of 100 were added for 2 h to 1×105 neutrophils seeded onto 0.01% poly-l-lysine (Sigma-Aldrich) treated 96-well black cell culture plates with the optical bottom ...
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bioRxiv - Immunology 2024Quote: ... The samples were blocked for 1 h at room temperature in PBS containing 2% bovine serum albumin (BSA, Sigma-Aldrich). After additional PBS washing ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in digestion medium (RPMI supplemented with 2% iHS [Sigma], 1 mg/ml Collagenase XI [Sigma], and 10 U/ml DNase I [Sigma]). Following digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Bioengineering 2024Quote: ... A 1 wt% alginate solution was prepared in 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer (MES, Sigma Aldrich, M8250). Sulfonated N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2024Quote: ... The slices were diced into 1 mm pieces and then treated with 10 mM tris(2-carboxyethyl)phosphine hydrochloride (SIGMA) at 56°C for 30 minutes for reduction ...
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bioRxiv - Cell Biology 2024Quote: ... each chamber contains 2 mL of respiration buffer (90 mM KCl [P4504, Sigma], 50 mM MOPS [M1254, Sigma], 1 mM EGTA [E4378, Sigma] ...
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bioRxiv - Microbiology 2024Quote: ... were determined using the standard microbroth dilution protocol68 and interpreted using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines.1 Cells were grown in Mueller Hinton Broth 2 (MHB2) (Millipore) and the inoculum size was set to 5 x 105 bacteria per ml ...
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bioRxiv - Cell Biology 2020Quote: ... blocking was performed in 3% BSA in PEM80 for 45 minutes and incubated overnight with a primary antibody against αTub (mouse IgG1, Sigma Aldrich, B-5-1-2, dilution 1:500). The cells were again washed with PBS (3×5 min ...
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bioRxiv - Biophysics 2023Quote: ... cells were resuspended in the appropriate amount of maintaining medium (RPMI Gibco® supplemented with 10% FBS, 2 mM GlutaMAX Sigma®, 1% Penicillin/Streptomycin Sigma®, 1 mM Sodium Pyruvate Gibco) and plated to allow maintenance and amplification ...
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bioRxiv - Bioengineering 2023Quote: ... was added to 1 mL of BG-11 in a 2 mL Eppendorf tube and supplemented with either 1 mM theophylline (Sigma-Aldrich; 100 mM stock dissolved in 100% DMSO) or 1% DMSO as a control and allowed to incubate for 3 days under 50 μmol photons m-2 s-1 prior to conducting the ABTS assays ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ml of 2% L-cysteine solution (2 g L-cysteine (Sigma Aldrich, #168149-100G) in 100 ml 0.1% MMR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 150 μL 2× Laemmli SDS-PAGE loading buffer supplemented with 10% 2-mercaptoethanol (Sigma-Aldrich) were added ...
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bioRxiv - Plant Biology 2021Quote: ... lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone) were purchased from Sigma-Aldrich. Vismione H and madagascine were obtained from PGE2 fraction of Psorospermum glaberimum as previously described (Gallé ...
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bioRxiv - Neuroscience 2021Quote: ... a selective neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) hydrochloride (Sigma, Dorset, UK) was used 3,4 ...
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bioRxiv - Bioengineering 2021Quote: ... We added 2-amino-thioxanthone (17.2 μmol, 2 equ) in dimethylsulfoxide (DMSO, 0.1 mL, Sigma) to a solution of PA-Rho (8.6 μmol ...
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bioRxiv - Molecular Biology 2020Quote: ... Transduced organoids were selected with 2 rounds of 2 μg/ml of puromycin (Sigma-Aldrich) on day 2 and day 4 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-chloro-2’-deoxyuridine (CIdU) and 5-lodo-2’-deoxyuridine (ldU) were obtained from Sigma.
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bioRxiv - Biochemistry 2021Quote: ... and incubated with the 0.1mM substrate methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (Sigma) for 1h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then pre-treated with 2mM 2-deoxy-D-glucose (2-DG) (Sigma-D6134) for 15 minutes prior to the assay ...
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bioRxiv - Developmental Biology 2022Quote: ... 2.5 μM Oligomycin A (BioShop) or 50 mM 2-Deoxy-D-glucose (2-DG, Sigma) for 24 hours ...
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bioRxiv - Physiology 2022Quote: ... 0.1 M N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) sodium salt (Sigma-Aldrich, H7006), 0.12 M sodium chloride (Sigma-Aldrich) ...
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bioRxiv - Physiology 2023Quote: ... For sectioned tissues (Figure 2, Supplementary Figure 2) samples were incubated in 30% sucrose (Sigma) overnight (for brains 5 days) ...
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bioRxiv - Immunology 2023Quote: ... these cells were pre-treated or not with 2-bromopalmitate (2-BP, Sigma, 21604-1G) at 50 μM for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... Triplicates of bacterial overnight cultures in Mueller-Hinton broth 2 (MHB 2, Sigma-Aldrich, #90922) were prepared from three different colonies the day before and grown shaking at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was collected and prepared for glycogen measurement alongside a 2 mg/mL glycogen standard using the Sigma glycogen assay kit (Catalog No. MAK016, Sigma, St. Louis, MO, USA) and read using a BioTek Synergy HT plate reader at 570 nm.
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Immunology 2021Quote: ADCP was performed using CHO cells stably expressing full-length wild-type SARS-CoV-2 S glycoprotein (target cells) fluorescently labelled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich; Cat. Nr.: MINI67). Target cells were incubated with titrated concentrations of mAbs for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biophysics 2021Quote: ... 10 units of Glucose-6-phosphate Dehydrogenase from baker’s yeast (G6PDH; S. cerevisiae; Sigma-Aldrich) and either 0 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected after 48 h using 6 µg/ml Blasticidine S (Sigma Aldrich) for 2 weeks.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 10 μM 6-Diazo-5-oxo-L-norleucine (DON, Sigma Aldrich), 1 μM Palbociclib (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... cdc12-6 cultures were shifted to 37°C and treated with 200 mM hydroxyurea (Sigma) for 1 h to protect cells from subsequent phototoxicity during imaging (Howell et al. ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... 4% PFA fixed zebrafish embryos were embedded in 6% low-melting agarose (LMA, Sigma-Aldrich) dissolved in 0.1% Tween-20 (Sigma-Aldrich ...