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Citations for Millipore Sigma :
5351 - 5400 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... the supernatant was removed by aspiration and the nuclear pellet was resuspended in 200 uL staining mix (2% normal goat serum, 0.1% BSA, 1:500 anti-NeuN conjugated to AlexaFluor488 (Millipore, cat#: MAB377X) in PBS ...
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bioRxiv - Cell Biology 2021Quote: Mission plasmids directing expression of shRNAs targeting PlexinA2 (TRCN0000061499 (ShPlexA2#1) and TRCN0000061501 (ShPlexA2#2)) or PlexinA4 (TRCN0000078683) were purchased from Sigma Aldrich. The production of the lentiviruses ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... Both pre-gRNA- and gRNA-based spacers were designed as two separate oligos (Supplementary tables 1-2) and synthesized by Sigma Aldrich. The oligos were annealed ...
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bioRxiv - Bioengineering 2022Quote: ... 0.2 μL of reverse primer (both at 10 μmol L- 1) and 7.6 μL of molecular grade water (Sigma Aldrich, USA). Three qPCR technical replicates were prepared for each sample ...
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bioRxiv - Bioengineering 2022Quote: ... HA-TBA was dissolved in anhydrous DMSO (2 wt%) with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Cell Biology 2022Quote: ... HESC were treated with differential medium (DMEM/F12 with 2% CS-FBS) containing 1 μM Medroxyprogesterone 17-acetate (MPA, Sigma, M1629), and 0.5 mM dibutyryl cAMP (dbcAMP ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250). Lysates were separated on 4-12% Bolt™ Bis-Tris gels (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Newly eclosed adults were kept for an additional 1–2 days before inducing transcription activation by placement on food containing the steroid hormone mifepristone (RU-486; Sigma M8046) in a 25 μg/ml concentration and flipped every 2 days thereafter ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the tag was performed in 2-3 ml with 20 μl 3C protease (1 mg/ml, Sigma-Aldrich, SAE0045) added and incubated over-night at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... (60 mg, 0.23 mmol, 1 eq.), sodium pyruvate (46 mg, 0.345 mmol, 2 eq.) and Neuraminic5Ac aldolase (Sigma Aldrich EC 4.1.3.3) from E ...
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bioRxiv - Systems Biology 2022Quote: ... 50 mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The heart was excised and sectioned in 2-mm slices and then stained with 1% triphenyl tetrazolium chloride (TTC) (Millipore Sigma) in PBS solution at 37°C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... the mixture was transferred into 15 ml centrifuge tube and 2 ml 1% bovine serum albumin (BSA; Sigma, USA, cat# A4628) was added to terminate dyeing ...
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bioRxiv - Physiology 2023Quote: ... into the new glass vial with a PTFE cap (60940A-2, Fisher) filled with 25 μL of 1 μg/μL (-)-Nicotine (N3876-100ML, Sigma Aldrich), for a total of 25 μg of added nicotine ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at room temperature with a combination of 2 different primary antibodies: a mouse monoclonal anti-PCNA antibody (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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Complement receptor C5aR1 signaling in sensory neuron-associated macrophages drives neuropathic painbioRxiv - Pharmacology and Toxicology 2022Quote: ... or PSNL mice and incubated in a solution containing 1 ml of DMEM medium and 2 mg/ml of collagenase type II (Sigma-Aldrich) for a 1 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2024Quote: ... 1×10^6 POE SHSY5Ys were reverse transduced with the 1:2 lentivirus supernatant dilution in the presence of 10 µg/ml polybrene (Sigma Aldrich). Bulk populations of cells stably expressing mitoSRAI were established through selection with 1ug/ml puromycin (M P BIOMEDICALS UK) ...
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bioRxiv - Systems Biology 2024Quote: ... The lysate was centrifuged at 20,000 × g for 1 hour and the supernatant was then mixed with 2 ml anti-FLAG M2 affinity agarose (Sigma-Aldrich) overnight at 4 ⁰C with rotation ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... barcode set in suspension using identical concentrations for 2 hours at room temperature before 2x washes with GSH-CSB (1 mM Gulutathione (Sigma #G6529)) ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
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bioRxiv - Microbiology 2024Quote: ... Donor and recipient cells were mixed to a ratio of 10:1 and 2 µL of the mating was spot into a microscope slide containing an M9 (M9 salts (Sigma-Aldrich), 500M mM MgSO4 ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were cleared using a 1:2 mixture of Benzyl Alcohol: Benzyl Benzoate (108006, B6630, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2023Quote: ... samples were dehydrated in series of Methanol and cleared using BABB (a mixture of 1 part benzyl alcohol to 2 parts benzyl benzoate; Sigma-Aldrich, benzyl alcohol ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in 100% methanol for 1.5 h and cleared in BABB (1:2, benzyl alcohol:benzyl benzoate) (Sigma-Aldrich, 108006, B6630).
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bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... the respective drugs were serially diluted (1 in 2) in liquid YPD with the highest concentrations for myriocin (Sigma Aldrich, M1177) at 128 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... albicans yeast at the ratio of 1:20 in RPMI 1640 medium containing 2% heat-inactivated mouse serum (#S3509, Sigma-Aldrich) at 37°C for 3 h.
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos and larvae were raised until 5 days post fertilization (dpf) in E3 medium containing methylene blue or in 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) if prevention of pigment formation was required ...
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bioRxiv - Biophysics 2023Quote: ... bacteria were grown in isotope-enriched M9 minimal media containing 1 g/l of 15N-ammonium chloride and 2 g/l of 13C-glucose (Sigma, USA) supplemented with 1.7 g/l YNB (Yeast Nitrogen Base ...
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bioRxiv - Cell Biology 2023Quote: ... The washed nuclei pellets were resuspended in sorting buffer (2% BSA/RNase-free PBS) and labeled with anti-PCM1 (1:500, HPA023374, Sigma-Aldrich) on ice for 45 minutes ...
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bioRxiv - Microbiology 2023Quote: Synechococcus WH7803 wild type and mutant cultures were grown in constant light at 12 μmol quanta m−2 s−1 in Pro99 media amended with high molecular weight chitosan or colloidal chitin (Millipore Sigma) to a final concentration of 56 μg/ml in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... to 20 µl of 60S subunits (blasticidin S dataset) and 1 µl of 2 mM water-solubilised cycloheximide (Sigma-Aldrich 01810) to 20 µl of 60S subunits (cycloheximide dataset) ...