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Citations for Millipore Sigma :
5201 - 5250 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer (1xPBS with 0.05% Tween 20 (Sigma-Aldrich)) and incubated with 170 μL per well blocking buffer (1xPBS with 2% BSA and 0.05% Tween20 (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... chick embryos sections and open-book spinal cords were blocked in 6% BSA (A7906, Sigma) and 0.5% Triton (T9284 ...
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bioRxiv - Neuroscience 2021Quote: ... MK-801 and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) all purchased from Sigma-Aldrich, also with AR-C155858 and (S)-3,5-Dihydroxyphenylglycine hydrate (DHPG ...
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bioRxiv - Neuroscience 2022Quote: Unilateral dopamine lesions were induced by the administration of 6− hydroxydopamine hydrobromide (Sigma, H116-5MG) into the MFB at the following coordinates ...
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bioRxiv - Developmental Biology 2022Quote: ... uterine tissue was physically lysed using a Beadbug 6 Microtube Homogenizer (Sigma-Aldrich, cat#: Z742682) and the lysate was spun through a QIAshredder column (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Antigen retrieval was performed by heating the slides in 1x Citrate buffer pH 6 (Sigma) for 40 min ...
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bioRxiv - Neuroscience 2024Quote: ... 6-8 slices per animal were transferred to cell culture inserts (Millipore #PICM 030 50) and maintained in tissue culture media (Minimal Essential Media + 1 mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... with 50 μl of 10 μg/ml Glucose-6-Phosphate Isomerase (SIGMA, Cat. No. P5381) at 4°C overnight ...
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bioRxiv - Genomics 2023Quote: ... Media was exchanged after 6 hours and supplemented with 1.5 mM caffeine (Sigma-Aldrich C0750) to increase viral titer ...
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bioRxiv - Immunology 2023Quote: ... Mice were gavaged with a 0.25 mL solution with 6% carmine red (C1022, Sigma-Aldrich), 0.5% methylcellulose (M0512 ...
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bioRxiv - Immunology 2023Quote: ... Beads were washed 6 times with ice-cold RIP wash buffer (Millipore, cat#17-700). RNA was extracted using RNAzol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Another 6 ml of dilution buffer and 250 μL anti-FLAG M2 magnetic beads (Sigma) were added to the supernatant and the sample was incubated at 4 °C for 2 hours with constant rotation ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow cells were transduced with retroviral supernatants supplemented with Polybrene (6 µg/mL, Sigma) by spinfection ...
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bioRxiv - Genomics 2023Quote: ... and one set was treated with 1.5 uM 6-TG (Sigma Aldrich, Cat. No. A4882) for 4 days to screen for cells with MLH1 successfully knocked out ...
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bioRxiv - Cancer Biology 2023Quote: ... and performed tyramide signal amplification by adding 6 µg/mL tyramide-biotin (Millipore Sigma, SML2135) in amplification buffer (0.003% Hydrogen peroxide in 0.1× borate buffer) ...
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bioRxiv - Cancer Biology 2023Quote: Sub-confluent cultures of PC3 cells were treated cisplatin (6 μM; Millipore Sigma, #232120-50MG), docetaxel (5 nM ...
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bioRxiv - Cell Biology 2023Quote: ... followed by adding 6 μl decylubiquinone (10 mM, D7911, Sigma-Aldrich, St. Louis, MO, USA) to start the reaction ...
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bioRxiv - Microbiology 2023Quote: ... broth and agar included 25 µg/mL glucose-6-phosphate (Sigma-Aldrich, USA, Product #10127647001). Plates were maintained at 37°C overnight for Mu208 and for 36-60 hours for JC8.
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bioRxiv - Immunology 2023Quote: ... the peroxidase substrate ABTS (2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt, Sigma, A1888) was added and the absorbance determined at 405nm ...
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bioRxiv - Molecular Biology 2023Quote: ... the differentiation media was supplemented with 6 μM all-trans retinoic acid (RA, Sigma-Aldrich) until day 8 ...
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bioRxiv - Immunology 2023Quote: ... C57BL/6 mice were injected twice weekly with CCl4 (1.0 ml/kg, Sigma-Aldrich, USA) in olive oil (Bertolli) ...
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bioRxiv - Biophysics 2023Quote: ... The labelling reaction was layered on a 6-30% OptiPrep (Sigma-Aldrich, MilliporeSigma, Burlington, MA) density gradient and ultracentrifuged at 35000 rpm for 1 h at 4°C ...
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bioRxiv - Genetics 2023Quote: ISE6 cells (3×106 cells per well) were plated in 6-well plates (Millipore Sigma) and stimulated accordingly ...
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bioRxiv - Molecular Biology 2023Quote: ... cardiac perfusion was performed with 6 mL of cycloheximide (100 μg/ml in PBS, Sigma). Harvested tissues were immediately placed in a lysis buffer consisting of 1% Triton X-100 ...
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bioRxiv - Immunology 2024Quote: ... were thawed into BSK-H complete medium with 6% rabbit serum (50 mL, Sigma-Aldrich) or BSK-II complete medium with 6% rabbit serum (50 mL ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed in 10% DMSO + 6% normal donkey serum (NDS; EMD Millipore S30) + 0.2% Triton X-100 in PBS at 37°C for 2–3 d to block non-specific antibody binding ...
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bioRxiv - Biochemistry 2024Quote: ... N-(3-oxododecanoyl)-L-homoserine lactone (AHL-C12) and 6-methoxyflavanone were purchased from Sigma. Stock solutions of these compounds (DPH ...
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bioRxiv - Neuroscience 2024Quote: ... Stereotaxic injection of 6-OHDA-HCl (H4381 Sigma, 7.2 µg/µl in 0.02% ascorbate-saline) targeted the medial forebrain bundle (MFB ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were differentiated into DCs over 6 days by culturing in RPMI (Sigma-Aldrich) supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were gavaged with 100μl of sterile carmine red dye (6% w/v) (Sigma, C1022) dissolved in 0.5% methylcellulose (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... 6 or 8 h with 500 µM Indole-3-Acetic Acid (IAA, Sigma-Aldrich #I5148). For induction of Oct4 knockdown in ZHBTc4 cells ...
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bioRxiv - Neuroscience 2024Quote: ... organoids were maintained in Essential 6 medium supplemented with DM (2.5 µM, Sigma-Aldrich, P5499) and SB (10 µM ...
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bioRxiv - Microbiology 2024Quote: BALB/c mice were intraperitoneally injected with 200 μl of phenylhydrazine (6 mg/ml; Sigma). Three days after treatment ...
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bioRxiv - Microbiology 2019Quote: ... The product was purified from 1% agarose gel with GenElute Gel Extraction Kit (Sigma-Aldrich) and cloned into pJET1.2/blunt vector using CloneJET PCR Cloning Kit (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We used an in–house ELISA in which the plate was coated with individual sera and biotinylated MS-Hu6 was captured by streptavidin-HRP (Millipore, Catalog # 18-152). In a final experiment ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RBD (2.5 µg/mL) was adsorbed overnight at 4°C to a 384-well Nunc MaxiSorp ELISA plate (Millipore Sigma, St. Louis, MO). The RBD was removed via aspiration and the plate blocked with 3% BSA in TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in serum IgG free RPMI-1640 (Sigma-Aldrich, MO, USA) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo) containing 143 mM 2-Mercaptoethanol (Sigma-Aldrich). Western blotting using the elutes from affinity purification and the pre-purified input lysates were performed as described above with the following primary antibodies ...
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bioRxiv - Biophysics 2021Quote: HeLa H2B-mCherry day 2 aggregates were equilibrated for 1 h in CO2 independent medium containing 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich) and 1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... For the inhibition of Thymidylate kinase (DTYMK) cells were incubated for 72 h after adding 2 μM (final conc.) of the YMU-1 compound (Sigma-Aldrich), solved in 15 % DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... two CSNK1D targeting shRNA constructs were selected from the Mission TRC shRNA library (TRCN0000023769, named shCSNK1D#1; TRCN0000361946, named shCSNK1D#2; Sigma-Aldrich); non-targeting scrambled shRNA served as control (shc002 ...
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bioRxiv - Cancer Biology 2021Quote: ... prior to lysis in ChIP lysis buffer (20 mM Tris-HCl, 150 mM NaCl2, 2 mM EDTA, 1% IGEPAL CA-630 (Sigma-Aldrich) and 0.3% SDS in water) ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Genetics 2021Quote: ... Telomere length was measured by two orthogonal methods: 1) qPCR8 and 2) telomere restriction fragment (TRF) analysis using TeloTAGGG Telomere Length Assay (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed cells were permeabilized with PBS containing 0.2% Triton X-100 for 10 min and blocked with PBS containing 1% IgG-free bovine serum albumin (BSA) (Sigma, A2058) for 30 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... hiPSC-CMs were maintained in RPMI 1640 with HEPES and Glutamax (Life Tech, Bleiswijk, the Netherlands) with 2% B27 with insulin (Life Tech) and 1% penicillin/streptomycin (P/S, Sigma-Aldrich). Six days before mechanical stimulation ...