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Citations for Millipore Sigma :
5051 - 5100 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 1 mg of as-synthesized SWCNT powder was added to 2 mg of (GT)xx oligonucleotide (Integrated DNA Technologies) 1 mL of 0.1 M NaCl (Sigma-Aldrich). Each sample was ultrasonicated using a 1/8′′ tapered microtip for 30 min at 40% amplitude in an ice bath (Sonics Vibracell VCX-130 ...
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bioRxiv - Cell Biology 2024Quote: ... 1x Insulin-Transferrin-Selenium-A and 2 mM L-glutamine] supplemented with 1 μg/mL doxycycline (DOX; Sigma-Aldrich, # D9891), 10 μM SB431542 and 0.1 μM LDN193189 to promote a cortical fate ...
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bioRxiv - Cell Biology 2024Quote: ... extracts were produced as in70 without modifications and co-immunoprecipitation was performed by incubating 1-2 mg of nuclear extract with pre-equilibrated anti-HA Affinity Matrix (50 μl, Sigma) or agarose GFP traps (30 μl ...
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bioRxiv - Plant Biology 2024Quote: Auxin treatments were performed by immersing dissected SAM in 1 or 2 mM Indole-3-acetic acid (IAA) solution (Sigma) for five hours as described (Galvan-Ampudia et al. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were re suspended in FACS buffer (0.1% BSA in PBS and 2% FBS) containing 50µg/ml of DNase 1 (Sigma D-4513) to reduce clustering of cell samples ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells Glucose (10 mM) was first injected followed by Oligomycin (1 µM) then 2-Deoxy-D-glucose at 10 mM (Sigma). At least 30 wells were assayed for each cell type ...
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bioRxiv - Developmental Biology 2024Quote: ... with 1% fetal bovine serum (FBS) and 100 U/ml penicillin/streptomycin supplemented with 2 mg/ml collagenase (SCR103, Sigma) and 10 U/ml DNAse (D4263 ...
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bioRxiv - Microbiology 2024Quote: ... Activated macrophages were inoculated with Leptospira at a multiplicity of infection (MOI) of 100 bacteria-per-cell during 2 hr following by 1 hr of gentamicin treatment (Sigma) at 100 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested and pelleted before being suspended in a formaldehyde-reversal lysis buffer containing 500mM Tris + 150mM NaCl + 1% Triton X-100 + 2% SDS + 50mM 1,4-dithiothreitol (DTT) + 1X cOmplete EDTA-free Protease inhibitor cocktail (Sigma-Aldrich), pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Biochemistry 2024Quote: ... Tspan12 siRNA duplexes (#1: 5’-GCUUAUCUUUGCCUUCUCCTT-3’ and 5’-GGAGAAGGCAAAGAUAAGCTT-3’; #2: 5’-AUGAGGGACUACCUAAAUATT-3’ and 5’-UAUUUAGGUAGUCCCUCAUTT-3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Fbp1F/F and Fbp1ΔHep mice that were HFD fed for 12 wk or NCD-8 wo mice were fasted for 12-14 h and then given 1 g/kg glucose or sodium pyruvate (2 g/kg, Sigma) by i.p ...
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Molecular Biology 2023Quote: A small fraction (2%) of each release sample was incubated overnight at 37°C with 1 mg/mL Proteinase K (Sigma), 4 mM CaCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with HRP-conjugated goat anti-rabbit antibody (1:10,000, Sigma-Aldrich, St. Louis, MO, USA). The signal was visualized by chemiluminescent detection using ECL.
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bioRxiv - Neuroscience 2022Quote: ... followed by TBS-Tx (2 × 5 min) and incubated with primary antibody (Rat anti-muscarinic M2 monoclonal antibody, unconjugated, clone m2-2-b3, 1:750, Millipore Cat ...
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bioRxiv - Pathology 2023Quote: ... cut open longitudinally and stained with a 2:1 mix of 0.5% Oil Red O in isopropanol (O1391, Sigma Aldrich) and deionized water for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... mesothelial cells were pelleted at 800g for 2 mins and resuspended in 50:50 v/v of RPMI (supplemented with 10% FBS, 1× penicillin/streptomycin, 2 μg/ml heparin (Sigma) and 1 μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Tetramethylammonium (TMA) oxalate solution was made by mixing a 2 to 1 molar ratio of aqueous TMA hydroxide (Sigma Aldrich) and ammonium oxalate monohydrate (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed three times in RPMI + 10% FBS and resuspended at 2 × 107 cells/ml in PBS supplemented with 0.4 μg ml−1 LPS (Sigma-Aldrich). DCs were injected (5 × 105 cells in 25 μl ...
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bioRxiv - Biophysics 2023Quote: ... the tissue is first allowed to fold into a spheroid in a regular HM and then transferred to the experimental setup embedding it in a soft-gel (1%-2% low-melting-point agarose-Sigma) prepared in HM and cooled down before the introduction of the sample to prevent damage of the tissue ...
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bioRxiv - Cell Biology 2023Quote: ... An equal volume of chilled FACS buffer (0.2 μm filter-sterilized 1x PBS, 2%(v/v) FBE (fetal bovine essence, Avantor),1% penicillin (Sigma, P7794), 1% streptomycin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... followed by the seeding of synchronized Pf3D7 ring stage culture at 1% parasitemia and 2% hematocrit in standard RPMI 1640 (Sigma)-based medium ...
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bioRxiv - Cell Biology 2023Quote: ... Penis and lymph nodes were dissected and fixed for 1.5-2 h in 3% paraformaldehyde (penis) or overnight in 1% paraformaldehyde (PFA, Sigma, P6148) at 4°C (lymph nodes ...
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bioRxiv - Biophysics 2023Quote: ... The protein was eluted at 1.5 ml/min using the following gradient- 0 - 1 minute: 10% buffer 2 (filtered Acetonitrile (Sigma-Aldrich) with 0.1% TFA) ...
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were concentrated to 2 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 300-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2023Quote: ... were obtained by PCR with the primers P1-P2 and P2-P4 respectively (Supplementary Table 2) and cloned into pETDuet-1 (Novagen) using the In-Fusion HD Cloning system (Clontech) ...
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bioRxiv - Immunology 2023Quote: ... B cells were suspended at 2×106 cells per milliliter and rested in RPMI medium containing 1% low-lipid BSA (SRE0098, Sigma) at 37 °C for 1 h ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Cell Biology 2023Quote: For LC-MS/MS, proteins were reduced and alkylated in SDC buffer (1% Sodium deoxycholate, 40 mM 2-Cloroacetamide (Sigma), 10 mM TCEP (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos older than 24 hpf were transferred into E3 medium containing 0.003% 1-phenyl 2-thiourea (PTU, Sigma-Aldrich P7629). Animal work was approved by the local Animal Welfare and Ethical Review Body (AWERB ...
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bioRxiv - Cell Biology 2023Quote: ... composed of Egg yolk L-α-Phosphatidylcholine (EyPC, Avanti Polar), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, Avanti Polar) and Cholesterol (Sigma) (50:20:30 ...
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bioRxiv - Cell Biology 2023Quote: ... Whole-mount immunostained embryos were mounted either in 1:2 Benzyl Alcohol:Benzyl Benzoate (BABB, Sigma-Aldrich cat# B6630 and 305197) clearing mixture or Vectashield (VectorLabs ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.3% NP-40) containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA), followed by clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
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bioRxiv - Cancer Biology 2023Quote: ... Clonal cell lines were selected by pre-determined concentration of puromycin (0.5, 1 and 2 µg/ml; Sigma, Cat# P8833) for respective cell line and overexpression of desired protein was confirmed by Western blotting.
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bioRxiv - Neuroscience 2023Quote: ... larvae were serially dehydrated in 50% methanol and 100% methanol for clearing with BABB (Benzyl alcohol:benzyl benzoate 1:2, Sigma-Aldrich) for 24 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: Aliquots from washed and equilibrated (in 1 x degradation buffer) ADP-ribosylated (1 μl) and RNAylated (2 μl) rS1 (32P) were treated with 0.5 U endonuclease P1 (Sigma-Aldrich) 4 or 0.95 μM ARH1 or ARH3 (human ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each sample was lysed in 500 μL of lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, 1x protease inhibitor cocktail (Sigma-Aldrich)) for 20 mins at RT before heating to 99 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated on ice for approximately 1 h before the addition of Amberlite XAD-2 beads (Sigma-Aldrich). The reconstitution mixture was incubated at 4°C overnight on an orbital shaker.
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bioRxiv - Cell Biology 2023Quote: ... permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Cell Biology 2023Quote: ... [Ca2+]i responses to 80 mM KCl and 2) immunofluorescence in cells stained with smooth muscle-specific a-actin (SMA; 1:400, A2547, Sigma) and calponin (1:100 ...
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bioRxiv - Biophysics 2023Quote: ... the acrylate functionalized photodegradable monomer was synthesized by suspending 4-[4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy]butyric acid (0.0166 mol, Sigma-Aldrich) in anhydrous DCM (90 mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1h at room temperature with primary antibodies diluted in 2% fatty acid free BSA: anti-FLAG (1:1,000; Clone M2, Sigma, F1804), anti-Rubicon (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviruses were generated by transfecting 3 x 106 HEK-293T cells in antibiotic-free medium with 6 µg of total DNA in a 1:2:3 ratio of pMD2.G:psPAX2:transfer plasmid using polyethylenimine (PEI) (Sigma-Aldrich). 48 h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellet were lysed in 500 µL of freshly prepared lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF and protease inhibitor cocktail (Sigma-Aldrich)) ...