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Citations for Millipore Sigma :
5001 - 5050 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... BAC clone (RP11-293M10) (CHORI) was fluorescently labeled using Cy3-dUTP (ENZO) and nick translation kit according to the manufacturer’s instructions (Sigma-Aldrich). Human Cot-1 (DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... After 9 days 3-5 × 106 cells from selection conditions and 100 × 106 non-selection conditions were washed 3 times with PBS and pelleted by centrifugation for genomic DNA extraction using GeneElute Mammalian Genomic DNA Miniprep kit (Sigma) and Quick-DNA Midiprep Plus kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... with thiobarbituric acid to form a colorimetric product by using a lipid peroxidation assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the reaction product was examined at 532 nm ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Genomics 2020Quote: Between 250 ng and 2 µg of gDNA extracted from each tissue sample was bisulfite treated with an Imprint DNA Modification Kit (Sigma). Control samples lacking DNA template were run to ensure that there was no contamination during the bisulfite conversion of DNA ...
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bioRxiv - Genetics 2021Quote: ... were ground to a powder under liquid nitrogen and RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Genetics 2020Quote: Bisulphite conversions were carried out using the two-step modification procedure of the Imprint® DNA Modification Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ChIP assay was used to detect the enrichment of HDAC3 in the promoter region of the miR-17-92 cluster and that of p65 in the Pgf promoter region using the EZ-Magna ChIP TMA kit (Millipore). The lung fibroblasts of each group in logarithmic growth phase were cross-linked and cultured with 1% formaldehyde for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... E11.5 embryos were dissected into ice-cold PBS and associated yolk sacs used for rapid genotyping using the Extract-N-Amp Tissue PCR kit as recommended by the manufacturer (Sigma). During genotyping ...
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bioRxiv - Developmental Biology 2021Quote: ... The medium was changed every two days for seven days before detection of alkaline phosphatase-positive colonies (Alkaline Phosphatase detection kit, Merck-Millipore). Alkaline phosphatase-positive colonies were analyzed and quantified using ImageJ analysis software (81).
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was extracted from single blastocysts by placing each blastocyst in a microtube containing 4.4 µl Extraction buffer (REDExtract-N-Amp Tissue PCR Kit, Millipore Sigma) mixed with 1.1 µl of Tissue Prep Buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... The homogenate was heat inactivated at 70 °C for 5 min and then centrifuged at 14000 rpm for 3 min. Serum triglyceride determination kit (Cat. # TR0100) from Sigma was used to quantify triglyceride levels and protein levels were measured using the Quick Start™ Bradford 1X Dye Reagent (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted from 5 individual embryos at mid-gastrula (st. 11) or later employing the GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). The genomic region containing the CRISPR / Cas9 targeted region was PCR amplified using a nested PCR approach (Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... The activity of creatine kinase (CK) and lactate dehydrogenase (LDH) in plasma were measured using creatine kinase activity assay Kit (Sigma) and LDH activity assay kit (sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The ROS assay was performed using a fluorometric intracellular ROS kit according to the supplier’s protocol (Sigma-Aldrich, Diegem, Belgium). In brief ...
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bioRxiv - Neuroscience 2020Quote: The mouse cortex between P0 and P2 was collected and dissociated with papain kit and DNase I (#LK003176, Worthington; 250U/ml; #D5025, Sigma). OPCs were purified by immnopanning approach according to our previous protocol (Lang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... collected by centrifugation and superoxide anion production was determined by flow cytometry with Muse® Oxidative Stress Assay Kit (Millipore), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL samples were used in a colorimetric assay according to the manufacturer’s protocol (NAD/NADH Quantitation Kit, Sigma-Aldrich) as described 21.
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bioRxiv - Molecular Biology 2021Quote: PLA was performed on cultured granulosa cells using the Duolink PLA Probes and PLA Fluorescence in situ Detection Kit Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: T7-Pep2,32 a complete human proteome library was restriction cloned into the T7-SEPARATE vector using the EcoRI/XhoI sites.32,33 The library was packaged in vitro using the T7Select Packaging Kit (Millipore Sigma) and expanded by plate lysate amplification to obtain an average clonal representation of ~100 plaques per peptide ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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bioRxiv - Molecular Biology 2020Quote: Lactate levels in the culture medium were measured using Lactate Assay Kit (Sigma-Aldrich, Cat#MAK064, Saint Louis, MO, USA) according to manufacturer’s instructions ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
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bioRxiv - Molecular Biology 2019Quote: ... Black Gold staining was conducted according to the manufacturer’s instructions (Black Gold II myelin staining kit, EMD Millipore, Billerica, MA). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50), treated with DNaseI on columns (Qiagen ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 ×106 cells were allocated per ChIP assay and prepared using the Magna ChIP A/G chromatin immunoprecipitation kit (Millipore). Cells were centrifuged (300g) ...
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bioRxiv - Cancer Biology 2019Quote: ... Hybridization was performed at 50°C O/N with the probe 1.6 kb fragment containing the sequence (TTAGGG)n63 labelled with Nick Translation Kit (Sigma-Aldrich) and developed according to62 ...
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bioRxiv - Microbiology 2019Quote: ... Total cellular phospholipid was measured in the samples prepared for the cholesterol assay using a phospholipid assay kit (MAK122, Sigma). Cholesterol concentrations were then normalized to phospholipid concentrations.
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bioRxiv - Neuroscience 2019Quote: Fish larvae at 5dpf were fixed in 4% PFA and the TUNEL assay was performed according to standard protocol using ApopTag Kit (Millipore). Briefly ...
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bioRxiv - Plant Biology 2019Quote: Glucose concentration of the spent media was determined spectrophotometrically with the commercial High Sensitivity Glucose Assay Kit (Sigma-Aldrich, U.S.). Prior to measurements ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of immunoprecipitate (IP) to Input was calculated as described in the Magna MeRIPTM m6A kit (17-10499, Millipore). mRNA controls (m6A RNA and unmodified RNA ...
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bioRxiv - Genetics 2019Quote: Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Rhodamine In situ Apoptosis Detection Kit; Millipore). Embryos were fixed ...
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bioRxiv - Pathology 2019Quote: ... treated with opsonized RBCs (1:10) and intracellular ROS was determined with a fluorometric intracellular ROS kit (Sigma-Aldrich, MAK142). Stained cells were visualized and captured using Leica DMI6000B with Cy5 filter set ...
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bioRxiv - Genetics 2021Quote: ... All plasmids to be used for transfection were purified with HP GenElute Midi or Mini kits (Sigma # NA0200 and NA0150).
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bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... Enrichment of the chloroplast from the leaf samples was performed using ± 2 cm2 of leaf tissue with the Chloroplast Isolation Kit (Sigma, CPISO-1KT After this the DNA was extracted using a modified C-Tab protocol (Doyle JJ ...
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bioRxiv - Cancer Biology 2020Quote: ... and DNA was extracted as per the protocol in the Actetyl-Histone H3 Immunoprecipitation (ChIP) assay kit (Millipore, Billerica, MA). Anti-acetyl-Histone H3 antibody was used for the immunoprecipitation (Millipore) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Insulin and glucose were measured in plasma samples using the Sensitive Insulin enzyme immunoassay kit (EZRMI-13K, Millipore, Billerica, MA) and Glucose GOD-PAP enzyme immunoassay kit (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted as per manufacturer instruction and genomic DNA was removed using the RNase-free DNase I Amplification Grade Kit (Sigma). All RNA samples were assessed for quality using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: Proximity ligation assay (PLA) was performed using the Duolink® In Situ Red Mouse/Rabbit kit (DUO92008, Sigma-Aldrich, UK) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Both the ADP/ATP ratio and the total cellular ATP content were measured using a bioluminescence-based ADP/ATP assay kit (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: The phosphatase activity of purified PP2A/B55α complexes was assessed using a commercial Ser/Thr phosphatase assay kit (EMD Millipore). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cortex was determined following the standard Ellman reaction principle using a commercially available AChE activity assay kit (Catalog no. MAK119, Sigma-Millipore). The Ellman assay [42] was used to measure ChE activity in each brain region using 5,5’-dithio-bis (2-nitrobenzoic acid ...