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Citations for Millipore Sigma :
4851 - 4900 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Viral nucleotides were randomly amplified using a modified version of the Whole Transcriptome Amplification Kit 2 (WTA2, Sigma Aldrich) as described in Conceicao-Neto ...
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bioRxiv - Genetics 2020Quote: Purification of fatty acids was performed using the fatty acids purification kit cat MAK174 (Sigma Aldrich, St Louis, MO) from 0,15 grams of BFT flour ...
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bioRxiv - Genomics 2020Quote: ... All iPSCs and cerebral organoids were tested negative for mycoplasma using the LookOut Mycoplasma PCR Detection kit (Sigma MP0035). iPSCs from all donors were passaged until they were confluent ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination check was carried out using a LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louis, MO, USA). Cells were validated by STR profiling.
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bioRxiv - Cell Biology 2020Quote: ... 100 ul culture media was collected and acetate level was quantified with Acetate Colorimetric Assay Kit (#MAK086-1KT, SIGMA) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... Cell cultures were examined periodically for bacteria contamination and tested for mycoplasma by LookOut Mycoplasma PCR Detection Kit (Sigma).
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bioRxiv - Molecular Biology 2021Quote: Human genomic DNA was isolated from HeLa cells using a GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich). Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were obtained after centrifugation for 1 hour at 20,000 x g and incubated with anti-FLAG M2 affinity gel (FLAG Immunoprecipitation Kit, Sigma). Bound proteins were eluted with 3xFLAG peptide solution.
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in P1 solution (accordingly to kit mannufacturer) and incubated with with 9U lyticase (Sigma-Aldrich) at 37 °C for 30 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... Template plasmid was digested using DpnI and the PCR products were purified using a PCR purification kit (Sigma-Aldrich). Subsequently ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Microbiology 2019Quote: ... viral RNA (vRNA) was extracted from 100 µl of supernatant using the GenElute mammalian total RNA kit (Sigma-Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Plasmid DNA was purified with a NucleoBond Xtra Midi kit (Cultech, 22740410.50) and resuspended in nuclease-free water (SIGMA). Other plasmids used were pCMV-GFP expressing GFP under the CMV promoter ...
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bioRxiv - Genetics 2021Quote: ... These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with 0.1% TBST three times for 15 minutes each and developed using ECL kit (Millipore). The blots were imaged on an Amersham Imager 600 and band intensity was quantified using Image J.
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation (RIP) experiments were performed using the Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (17-700, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: PLA experiments were done using Duolink™ II secondary antibodies and detection kits (Sigma-Aldrich, #DUO92002, #DUO92004, and #DUO92008) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: The ATPase activity of MutL or MutL(R-E) was measured by an ATPase/GTPase Activity Assay Kit (Sigma). The analysis was carried out with 5 μM protein in a 40 μL reaction mixture comprised of 20 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600) with a LightCycler 480 apparatus (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... The quantity of glucose produced was then measured using a Glucose assay Kit (Sigma-Aldrich, St. Louis, MO; #GAGO20). For all assays ...
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bioRxiv - Cell Biology 2020Quote: ... Absence of mycoplasma in cell lines was routinely checked by the VenorGeM Mycoplasma Detection Kit (Sigma, St. Louis, MO).
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bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... LPS (Escherichia coli serotype 0111: B4) and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... Subsequently the PLA protocol was followed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma) instructions ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Neuroscience 2021Quote: ... or Gad1 were generated by in vitro transcription using cDNA templates and RNA DIG- or FITC-labeling kits (Sigma, catalog # 11277073910 or 11685619910 ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: Nuclei from the 700mg of wildtype flower tissue were isolated using the Plant Nuclei isolation kit (Sigma, # CELLYTPN 1) conferring to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of each sample was used as input for the NAD/NADH Quantitation Kit (Sigma-Aldrich, cat# MAK037) according to the instructions provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were tested for mycoplasma every three months using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) and were authenticated by ATCC®.
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bioRxiv - Cell Biology 2022Quote: ... Chromatin shearing and immunoprecipitation were performed using EZ-Magna ChIP A/G Chromatin Immunoprecipitation Kit (Sigma, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Mitochondrial mass in whole liver was measured according to the manufacturer’s instructions using the Citrate Synthase Activity Assay Kit (Sigma) and normalized to protein concentration ...
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bioRxiv - Cancer Biology 2022Quote: Nuclei were isolated from 50,000 cells for each sample using Nuclei EZ prep-Nuclei Isolation Kit (Sigma-Aldrich, USA). The transposition reaction mix (25μl of 2X TD buffer ...
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bioRxiv - Bioengineering 2022Quote: ... the lipid oxidation and superoxide level assays were conducted according to manufacturer’s specifications (Lipid peroxidation assay kit, Sigma Aldrich Cat#MAK085 ...
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bioRxiv - Cell Biology 2022Quote: Total hydroxyproline content to quantify extracellular matrix deposited collagen was measured using a commercially available kit (#MAK008, Sigma-Aldrich) as previously detailed125 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were fixed and stained with Tartrate-resistant acid phosphatase (TRAP) kit according to the manufacturer’s instructions (Sigma) to quantify osteoclast numbers ...
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bioRxiv - Plant Biology 2022Quote: ... stems and roots of M15 plants grown outside under low light conditions using SpectrumTM Plant Total RNA Kit (Sigma). For differential gene expression analyses of secondary metabolite genes ...
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bioRxiv - Molecular Biology 2022Quote: Duolink™ proximity ligation assays were performed with the Duolink In Situ Red Starter Kit Mouse/Goat (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid library DNA was purified from twice 5 ml of culture using a GenElute Plasmid Miniprep Kit (Sigma-Aldrich) with elution in twice 20 µl of ddH2O ...
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bioRxiv - Microbiology 2022Quote: ... Size standards were generated using a gel filtration marker kit of protein molecular weights that ranges from 29,000 to 700,000 (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: We measured cytokine concentration in maternal and fetal plasma by Luminex technology using multiplex kits for nonhuman primate (Millipore). Concentrations were calculated from standard curves for recombinant proteins.
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bioRxiv - Molecular Biology 2023Quote: Drug efflux by MDR1 was measured with the Multidrug Resistance Direct Dye Efflux Assay kit (Merck Millipore; ECM 910) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The WTA product was performed using the Complete Whole Transcriptome Amplification Kit (WTA2)39 (Sigma-Aldrich, St. Louis, MO), with the PCR reaction then undertaken using a set of self-designed primer pairs based on the obtained reads ...
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bioRxiv - Neuroscience 2023Quote: ... It was further processed for the detection of glutamate release using glutamate determination kit (Sigma Aldrich, USA, Cat# GLN1) as per manufacturer’s protocol.
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bioRxiv - Physiology 2022Quote: Senescence-associated β-gal staining was performed with the Cellular Senescence Assay Kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Plant Biology 2023Quote: RNA extraction of Marchantia polymorpha and Physcomitrium patens was done using the Spectrum™ Plant Total RNA Kit (Sigma) and for Arabidopsis thaliana the NucleoSpin RNA Plant min kit (Macherey -Nagel ...
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bioRxiv - Molecular Biology 2022Quote: The content of hydroxyproline in ligament fibroblasts was measured using a hydrolysate of hydroxyproline assay kit (Sigma, MO, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and either lysed with RNA lysis buffer or fixed and stained using an Oil-red-O staining kit (Sigma) as per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell lines were verified to be free of mycoplasma contamination by Venor™ GeM Mycoplasma Detection Kit from Sigma.