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Citations for Millipore Sigma :
5051 - 5100 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... the pellets were collected to examine the cell lysis interference using a Glucose-6-Phosphate Dehydrogenase kit (Sigma-Aldrich, USA). The details of EPS extraction and analytical methods for various EPS components (proteins ...
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bioRxiv - Cell Biology 2021Quote: ... G6P concentrations were determined at 2 hrs and 6 hrs post activation and compared to unactivated controls using a G6P kit (Sigma) and following manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... 5% CO2 tissue culture incubators and routinely confirmed to be mycoplasma negative using LookOut mycoplasma PCR detection kit (Sigma Aldrich). pLifeAct-mScarlet-N1 and pLck-mVenus-C1 were gifts from Dorus Gadella (Addgene plasmids #85054 and #84337 ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Cell Biology 2021Quote: ... The PKH26:MYH10-KD co-cultures 3T3-L1 cells were labeled with 0.5λ PKH26 Fluorescent Cell Linker Kit (Sigma-Aldrich); the GFP cells were added after one day.
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were washed once in PBS and nuclei obtained using the EZ Prep nuclei kit then incubated with 50μM Azide-PEG3-biotin conjugate (Sigma-Aldrich) was dissolved in prewarmed Buffer A and added to samples. ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitates were applied to 8 % SDS-PAGE and the gel was stained by ProteoSilver™ stain kit (Sigma-Aldrich) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted from the in vitro samples using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, MO, USA) according to the manufacturers’ instructions ...
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bioRxiv - Genomics 2021Quote: The high-quality genomic DNA was isolated from all three strains using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression vectors for human Arf1 (UniProtKB: P84077) and Arf6 (UniProtKB: P62330) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described for protein expression of human Rab-family small GTPases (Tamura and Mima ...
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bioRxiv - Plant Biology 2021Quote: The coding sequences of most of the 44 non-pseudogene AtUMAMIT were amplified by PCR (oligonucleotide sequence is provided in Supplemental Table 1) by using the KOD Hot Start DNA Polymerase kit (Novagen) from a mix of reverse-transcribed RNA extracted from seedlings and inflorescences ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated at different time points using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). Aliquots with 50 ng RNA were subjected to DNAse treatment (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: 5′RACE (Rapid amplification of cDNA ends) assays were performed with total RNA extracted from UK1_O using a 5′RACE System kit (Sigma Aldrich), according to the instruction provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... PCR-positive colonies were grown and the vector was extracted using GenElute Plasmid Miniprep Kit following manufacturer’s protocol (Sigma-Aldrich). The vector was then transformed into expression strain E ...
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bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of DHFR with and without SCH-79797 treatment was measured using the Dihydrofolate Reductase Assay kit from Sigma. Briefly ...
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bioRxiv - Microbiology 2020Quote: ... transferred to DMEM (1000 mg/l) and media glucose concentration was measured over a 24 h period using a glucose assay kit (Sigma). Glucose levels were expressed relative to media collected from worms which received naïve IgG (negative control) ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... GP:NPC1 interaction was detected by applying the Duolink® In Situ Detection Reagents Red kit following the manufacturer’s instructions (Sigma). After removing excess reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting plasma supernatant was transferred to a sterile Eppendorf tube and stored at −80 °C until the cytokines were measured using a Milliplex xMAP Mouse High Sensitivity TCell Magnetic Bead Panel kit MHSTCMAG-70K (Millipore) following the kit protocol on a Luminex 200 system with Xponent (v2.7) ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole genome amplification was performed using SeqPlex™ Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac and H9c2 ATP content was determined using a the Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit (Sigma #FLAA-1KT), and normalized to DNA content as described previously 50 ...
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bioRxiv - Cell Biology 2021Quote: ... and lactate and pyruvate were measured using colorimetric assays using kits per manufacturer’s instructions (insulin, Cat no. RI-13K, Millipore Sigma, Burlington ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... pre-sorted or sorted cells were stained with either trypan blue or a Calbiochem live/dead double staining kit (Millepore Sigma) which uses a cell permeable green fluorescent Cyto-dye to stain live cells and propidium iodine to stain dead cells ...
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bioRxiv - Cancer Biology 2022Quote: Oleoyl-L-α-lysophosphatidic acid (LPA, L7260) and a Periodic Acid-Schiff (PAS) Kit (395B) were purchased from Sigma-Aldrich. The PKD inhibitor CRT0066101 (A8679 ...
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bioRxiv - Physiology 2022Quote: Cellular ATP levels in FACS-sorted RPMs (10 000 cells/sample) were determined using ATP Fluorometric Assay Kit (Sigma, MAK190), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... sensitivity calibration and standard curves were made on the basis of recommendations of the 20S Proteasome Activity Assay Kit (Millipore).
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bioRxiv - Plant Biology 2022Quote: ... total RNA was extracted from 14 day-old Arabidopsis thaliana seedlings using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) and treated with DNAase I (RapidOut DNA Removal Kit ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana seedlings for each line grown on a ½ MS agar plate using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... resuspended in RNA extract lysis buffer (100µL RLT lysis buffer from RNA extraction kit supplemented with 40mM DTT, Sigma, #D9779), vortexed and let sit (10min. ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# AS1107) for one hour at room temperature and visualized using the Immobilon ECL substrate kit (Millipore, Merck KgaA, Germany). Primary antibodies specific to PLLP (1:700 ...
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bioRxiv - Genetics 2022Quote: ... Cell death analysis was performed using the ApopTag® Fluorescein in situ Apoptosis Detection Kit (Sigma-Aldrich, Cat. No. S7110) following manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... obtained by grinding in liquid nitrogen and using a SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, DE) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cardiac tissues were fractionated into nucleus-enriched and cytoplasmic samples by using a CelLytic™ NuCLEAR Extraction kit (Sigma, Nxtract), as previously described (32).
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bioRxiv - Genetics 2022Quote: ... To assess editing efficiency genomic DNA was isolated from CRISPR-edited and control cells using a GenElute Mammalian DNA Miniprep Kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted from 100 mg of whole seedling tissue using the Spectrum Plant Total RNA Kit (Sigma, STRN50) and l μl of eluted RNA was used for complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... the PCR reaction described above was repeated using genomic DNA isolated using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... histolytica trophozoites were grown with and without queuine (0.1μM for three days) and total RNA was isolated using the TRI reagent kit according to the manufacturer’s instructions (Sigma-Aldrich). Three biological replicates from each treatment condition were processed ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Microbiology 2020Quote: ... validated as hex1 deletion mutants by spore PCR and Southern blot analysis (DIG DNA Labeling and Detection Kit, Sigma-Aldrich), and stored as 25% glycerol stocks at −80 °C.
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated by gently homogenizing tissue with a pestle in cold Nuclei Isolation Buffer from a Nuclei PURE Prep Kit (Sigma). Nuclei were pelleted and supernatant removed before being digested by incubating in 70 µl PBS ...
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bioRxiv - Genomics 2020Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 × 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Protein concentrations of purified luciferase enzyme variants (+4G, +4C, +4A, and +4T) were quantified using the Bicinchoninic Acid (BCA) kit by following manufacturer’s protocol (Sigma-Aldrich). Equal amounts of each luciferase variant was diluted in Passive Lysis Buffer (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... plasma was isolated from tail-blood and measured (3 μL in technical duplicates) using the Cholesterol Quantitation Kit (Sigma, MAK043) or the Triglyceride Quantification Colorimetric/Fluorometric Kit (Sigma ...