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Citations for Millipore Sigma :
5151 - 5200 of 5994 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Fluorescein In Situ Apoptosis Detection Kit; Millipore) as previously described (Lambert et al. ...
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bioRxiv - Neuroscience 2020Quote: APOE genotype was determined by extracting genomic DNA from participants’ blood samples using a GenElute Blood Genomic DNA Kit (Sigma). After confirming concentration and quality by Nanodrop ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 6000xg for 10min and the pellet was re-suspended in LB/kanamycin for plasmid extraction and purification using a GenElute plasmid miniprep kit (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... Experimental molecular weights were calculated using calibration standards from the Gel Filtration Molecular Weight Markers Kit for molecular weights 12-200 kDa (Sigma) and the Gel Filtration Calibration Kit for molecular weights 43-669 kDa (Cytiva) ...
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bioRxiv - Biochemistry 2021Quote: ... centrifuged for 5min at 500×g and LDH release was measured using the In Vitro Toxicology Assay Kit (Sigma-Aldrich) according to the manufacturer protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR fragments were analyzed using Agarose Gel Electrophoresis and purified from Gel using the GenElute Gel Extraction Kit (Sigma, NA1111) or MinElute Gel Extraction Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: Rat cortical neurons plated in 10cm2 dishes (approximately 10 million neurons per dish) were used with a commercially available Rho Activiation Assay Kit (Millipore). The neurons were placed in plain NB media for 4hrs prior to assay ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore #17-700) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Colorimetric measurement of imidazoquinoline interaction with P-gp was determined using a PREDEASY ATPase Assay Kit (SOLVO Biotechnology, Sigma-Aldrich) in 96 well plate format following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Glycaemia was measured using a glucometer and insulin levels using the Milliplex Map Kit (Mouse Metabolic Magnetic Bead Panel. MMHMAG-44K, Millipore) Milliplex Map Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Citrulline-containing proteins were modified on the membrane and detected using the anti-modified citrulline detection kit (Millipore, 17-347) according to manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Cells were harvested at μmax and genomic DNA was isolated using GenEluteTM Bacterial Genomic DNA Kit (NA2120-1KT, Sigma-Aldrich) using the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... CRISPR-targeted regions were amplified with MiSeq-compatible gene-specific primers containing Read1 and Read2 adaptor sequences (IDT) using KOD DNA polymerase Hot start kit (TOYOBO/Millipore). The second PCR was performed with index adaptor primers compatible with Ilumina MiSeq (i5 and i7 primers ...
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bioRxiv - Cancer Biology 2021Quote: Gene expression was measured by quantitative RT-PCR and normalized to β-actin8,48 The SYBR Green quantitative RT-PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Genomics 2019Quote: Whole genome amplification was performed using SeqPlex™ Enhanced DNA Amplification kit as described by the manufacturer (Sigma-Aldrich, SEQXE). Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Microbiology 2020Quote: IDH activity assays were performed using the Isocitrate Dehydrogenase Activity Assay kit (MAK062) from Sigma-Aldrich (St. Louis, MS, USA) and monitored with a Synergy HT microplate reader (BioTek ...
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bioRxiv - Microbiology 2020Quote: ... From these fixed cells the manufacturer’s Duolink PLA Immunofluorescence protocol was followed closely using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma). Briefly ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Neuroscience 2020Quote: ... They were blocked by incubating with Duolink blocking solution according to the manufacturer’s indications (Duolink® in situ PLA kit, Sigma). Cells were incubated with rabbit polyclonal anti-HA.11 and mouse monoclonal IgG1k anti-GFP antibodies at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... Ear homogenate supernatants were assayed for chemokines and cytokines with the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel kit (Millipore). Samples were collected and analyzed using BioPlex 200 reader (BioRad).
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split 48 hours after transfection and DNA was extracted using Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich #G1N350). Non-homologous repair efficiency was evaluated by Sanger Sequencing using KAPA2G Taq polymerase (Kapa Biosystems #KK5601 ...
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bioRxiv - Cell Biology 2021Quote: ... A DNA probe against the NST1 region within the rDNA was synthesized using the Fluorescein-High Prime kit (Sigma-Aldrich). The fluorescein-labelled probe hybridization was carried out overnight at 68 °C ...
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Whole mount TUNEL was performed as described previously (Causeret et al., 2011) using the Apoptag Peroxidase in situ Apoptosis Detection kit (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were processed using the EpiCypher CUTANA™ CUT&RUN Kit (SKU: 14-1048) per the manufacturer’s instructions with 0.5 μg/100 μl anti-Hoxa11 (Sigma-Aldrich SAB1304728) or anti-IgG (CUTANA™ SKU ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were boiled at 96°C for 5 minutes and then quantified with QuantiPro BCA Assay Kit (Sigma, Cat. SLBF3463). 40 μg of proteins were mixed with the right amount of Laemmli Sample Buffer 4X (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from the wild-type and bsu-q seedlings treated with flg22 or mock solution using the Spectrum Plant Total RNA kit (Sigma). cDNA libraries were constructed using a TruSeq RNA sample preparation kit (Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... These oligonucleotides were used to create a phage library representing all oligopeptides using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol (Harhala et al. ...
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bioRxiv - Cancer Biology 2022Quote: PC3 cell proliferation was quantified by an MTT based colorimetric assay using the Cell Proliferation Kit I (Roche, Sigma Aldrich). WT ...
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bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
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bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Immunology 2022Quote: ... Adenosine 5′-triphosphate (ATP) was assessed in the cell-free TA supernatants using bioluminescent assay kit following the manufacturer’s instructions (Sigma-Aldrich). The levels of soluble immune mediators were measured in TA samples using a high-throughput microbeads array (Bio-Plex Pro™ Human Cytokine 27-plex Assay ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was extracted from stressed and control plants (three biological replicates each) shown in Figure 4A using SpectrumTM Plant Total RNA Kit (Sigma). Quality control of all the extracted RNA (triplicates for each condition ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Microbiology 2022Quote: RNA extraction using Trizol-Total RNA was extracted using the TRI reagent kit according to the manufacturer’s instructions (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: Mutations in the heme-induced genes (HIGs) were constructed using the Targetron Intron Mutagenesis kit (Sigma-Aldridge, St. Louis, MO) as described previously [12] ...
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bioRxiv - Microbiology 2022Quote: ... The effect of GpsB on the GTPase activity of FtsZ and FtsZΔC6 was determined by measuring the free phosphate released using the malachite green phosphate assay kit (Sigma Aldrich). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Sizes were only referenced according to the Gel Filtration Makers Kit (for protein molecular weight 29 kDa to 700 kDa, Sigma).
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bioRxiv - Physiology 2022Quote: ... was measured on tissue homogenates and isolated CMs with a kit purchased from Sigma-Aldrich (MAK193; St. Louis, MO, USA). In brief ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from each tissue using the Spectrum Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO, USA) with 2% PVP40 added to the extraction buffer to sequester phenolic inhibitors ...
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bioRxiv - Physiology 2023Quote: ... a colorimetric-based enzyme assay was used to measure alanine aminotransferase (ALT) levels in peripheral plasma collected from the submandibular vein monthly using a Mouse ALT Activity Assay Kit (SIGMA). Serum LBP level was measured by a Mouse Lipopolysaccharide Binding Protein ELISA Kit (HyCult Biotechnology ...
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bioRxiv - Physiology 2022Quote: ... Mitochondrial content was measured using approximately 5-10 mg of tissue with a colorimetric citrate synthase activity kit (Sigma MAK193).
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Mycoplasma testing was performed monthly for all the lines included in this study at any differentiation stage (LookOut Mycoplasma PCR Detection Kit, Sigma). All cells used in this study were consistently negative.