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Citations for Addgene :
201 - 250 of 908 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The sequence of GeneSwitch was amplified by PCR using pElav-GeneSwitch (Addgene: 83957). The backbone vector pElav-GeneSwitch was digested with KpnI ...
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bioRxiv - Developmental Biology 2020Quote: MoxMaple3 was amplified by PCR from the pmCherry-T2A-moxMaple3 (Addgene Plasmid #120875) [43] and inserted into the pUASp by SpeI (3’)/EcoRI (3’) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplicons of sT and LT gene regions from plasmid RAZ1 (Addgene #114381) were used as target DNA templates (Refer Supplementary Table 1 for primers) ...
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bioRxiv - Genomics 2020Quote: ... PCR amplified and cloned into Esp3I-digested plentiGuide-Puro (Addgene plasmid ID: 52963) lentiviral vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... the natNT2 marker was PCR amplified from pUG-natNT2 (Addgene plasmid #110922, (26)) using primers 10297 and 10298 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the mEmerald tag was PCR amplified from mEmerald-PLK1-N-16 vector (Addgene #54234 ...
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bioRxiv - Bioengineering 2021Quote: ... Desired sgRNA sequence was PCR amplified using plasmid pICH86966::AtU6p::sgRNA_PDS (Addgene #46966) as a template and cloned together with either pJ23119 or pAtU6 for sgRNA expression.
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bioRxiv - Cell Biology 2021Quote: The KIF18B coding sequence was amplified by PCR from 24xMoonTag-kif18b-24xPP7 (Addgene plasmid #128604 [38] was a gift from Marvin Tanenbaum ...
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bioRxiv - Biophysics 2022Quote: ... Kinesin 1 1-401 (K401) was PCR amplified from pWC2 plasmid (Addgene # 15960). Ncd 236-701 was PCR amplified from a plasmid gifted by Andrea Serra-Marques.
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bioRxiv - Microbiology 2020Quote: The SNAP-tag gene was PCR amplified from pSNAP-tag(m) (Addgene #101135) and cloned into pVpr-IN.eGFP(Albanese et al. ...
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bioRxiv - Microbiology 2021Quote: ... The Cas9 part was generated via PCR amplification of pCas9/Decoy (Addgene #80324)8 using primers P12/P13 ...
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bioRxiv - Immunology 2020Quote: ... The mAID-Clover cassette was PCR amplified from the plasmid pMK290 (Addgene, #72828). These fragments together with a puro selection cassette amplified from pEN84 were assembled in an order diagramed in Extended Data Fig ...
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bioRxiv - Systems Biology 2021Quote: ... individual drivers were PCR amplified out of the Cancer Pathways kit (Addgene #1000000072)21 ...
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bioRxiv - Neuroscience 2020Quote: ... secA5-YFP sequences were amplified by PCR from pBH-UAS-secA5-YFP (Addgene), and subcloned into the pAAV-CAG-GFP (Addgene ...
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bioRxiv - Physiology 2021Quote: ... Fluorescent protein sequences were PCR-derived from pmVenus(L68V)-mTurquiose2 (Addgene plasmid #60493), Gamillus/pcDNA3 (Addgene plasmid #124837) ...
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bioRxiv - Biochemistry 2020Quote: ... The EMCV IRES sequence was amplified by PCR from EMCV_IRES_pcDNA4TO_H2B_SunTag24×_v1 (Addgene #246719) using the following primers ...
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bioRxiv - Cancer Biology 2021Quote: Scarlet-H2A was amplified using PCR (donor plasmid: Addgene #85051, from Dorus Gadella) and subcloned into pDONR221 using the Gateway BP Clonase II enzyme mix (#11789020 ...
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bioRxiv - Neuroscience 2021Quote: ... the fragments were amplified with PCR and then assembled in pHD-DsRed (RRID:Addgene_51434) with NEBuilder (New England BioLabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... human MEG3 cDNA was PCR amplified from the pCI-Meg3 (Addgene Plasmid #44727) using NEB Q5 high-fidelity polymerase ...
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bioRxiv - Molecular Biology 2021Quote: ... and the ~350 bp PCR product was cloned directionally into pFRB-NLUC (Addgene) cut with the BamH1 and BsiW ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The nourseothricin resistance gene (NAT) was PCR-amplified from pICH47732:FCP:NAT (Addgene #85984) using primers 1 and 2 (Table S5) ...
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bioRxiv - Neuroscience 2022Quote: ... a GFP-kanamycin marker was PCR amplified from a template vector (PL452, Addgene). The primers include homology arms consisting of 50 nucleotides upstream and downstream from the start codon of the vav gene ...
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bioRxiv - Cancer Biology 2023Quote: ... Myristolated AKT was amplified by PCR from pT3-myr-AKT-HA (Addgene #31789) and cloned into the pSBbi-w/o-Puro backbone ...
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bioRxiv - Cell Biology 2023Quote: ... BirA was PCR amplified from pDisplay-BirA-ER (Alice Ting lab, Addgene #20856) with AttB sites as described above and transferred to pDEST12.2 or pLenti-DEST-EF1A-Hygro ...
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bioRxiv - Cell Biology 2023Quote: ... 10 pmol of PCR repair template amplified from pSNAP-tag plasmid (Addgene #101135) or mTagBFP2 plasmid (Addgene #75029 ...
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bioRxiv - Biophysics 2022Quote: ... plasmids vector was obtained from circular PCR amplification of pBLO62.5 (Addgene plasmid #123124) with two primers respectively pairing to N-terminal and C-terminal NLS sequence (Tsuchida et al. ...
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bioRxiv - Systems Biology 2023Quote: ... CSE1L and NUP37 were cloned by PCR into pEN_TT_miRc2 3xFLAG-BirA* (Addgene #192307). Full cloning details are available in Supplementary Data 7 ...
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bioRxiv - Cell Biology 2023Quote: ... the RhoA biosensor fragment was PCR amplified from the pLentiRhoA2G plasmid (Addgene #40179) to add BamHI and XhoI sites at the ends ...
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bioRxiv - Synthetic Biology 2023Quote: ... inverse PCR from CMVp-dsRed2-Triplex-28-gRNA1-28 (Addgene cat no. 55200) using a pair of primers including SacI restriction sites generated a linear plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... were amplified by PCR and cloned into pLenti-GFP-puro (Addgene plasmids #17481) Lentivirus were produced by transforming pLenti constructs ...
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bioRxiv - Synthetic Biology 2023Quote: ... The first fragment was PCR amplified from pETM6-HCAmp-EV (Addgene Cat#49795)(P ...
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bioRxiv - Bioengineering 2023Quote: ... difficle genome by PCR and cloned into the 2Bc-T plasmid (Addgene #37236); Nluc ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... and the p10-3’UTR PCR amplified from the plasmid pJFRC81 (Addgene #36432). These were then cloned by Gibson assembly (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and ITSN1-tgRFPt-SspB were PCR-amplified from pLL7.0:Venus-iLID-CAAX (Addgene #60411 ...
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bioRxiv - Cancer Biology 2024Quote: ... full length BRCA1 was PCR-amplified from pCL-MFG-BRCA1 (Addgene #12341 (74)) with a triple Ty1-tag included in the reverse primer and subsequently cloned into pENTR_1A ...
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Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetamsbioRxiv - Biochemistry 2024Quote: ... Nb1-5 were PCR amplified and subcloned into the vector pBXNPHM365 (Addgene #11099) and fused with a C-terminal (TSII-tagged ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was PCR-amplified from one of our previously described plasmids (Addgene #132667)36 ...
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bioRxiv - Genetics 2024Quote: ... and 3’ UTR was PCR-amplified and cloned into the pGRB2.0 plasmid (Addgene) (73 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cas7-11 ORF was PCR amplified from pDF0229- DiCas7-11 (Addgene plasmid #172506) with Q5 High-Fidelity DNA polymerase (M0491 ...
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bioRxiv - Molecular Biology 2024Quote: pME LiCre (JDW 1119) was created by PCR amplification using pGY577 (Addgene #166663) (a gift of Gaël Yvert ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting PCR products were cloned into the lentiviral vector pHR_SFFV (Addgene, #79121) using the restriction enzymes EcoRI and XhoI (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... The synthetic insulators sIns1 and sIns2 were PCR amplified from pZS*11_4enh (Addgene no ...
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bioRxiv - Molecular Biology 2024Quote: ... The Ing4 cDNA was amplified using PCR from a plasmid template (Addgene #13289) using 5’-AGGCTCGAGATGGCTGCGGGGATG-3’ and 5’-AGAATGCATTTTCTTCTTCCGTTCTTGG-3’ primers and was cloned into the XhoI and NsiI sites using the Oct4/pFastBac-1 vector by first excising the Oct4 cDNA using XhoI and NsiI ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments were created by PCR using pGD-CMV-jGCaMP7c (Addgene plasmid #105320), pcDNA3-Cyto-GCaMP3 (Addgene plasmid #64853) ...
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bioRxiv - Developmental Biology 2022Quote: ... a solution containing two sgRNAs (40 ng/μL each) and Cas9 protein (250 ng/μL) (Addgene; 47327) (Gagnon et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Other gene deletions were generated using PCR products derived from pFA6a-kanMX6 (Addgene 39296) or pFA6a-hphNT1 (Euroscarf P30347 ...
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bioRxiv - Cell Biology 2020Quote: mCherry-ER fusion protein was amplified by PCR from mCherry-ER-3 plasmid (Addgene) and cloned into Gateway modified pBABE (in house) ...
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bioRxiv - Molecular Biology 2021Quote: ... emiRFP2 were PCR amplified and swapped with miRFP670nano in pKeratin-miRFP670nano plasmid (Addgene #127437) using Fusion cloning (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... crRNA linked with TRACR (sgRNA) were amplified by PCR with a pLKO vector (Addgene_52628) as template ...
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bioRxiv - Genetics 2020Quote: ... Gal4 5’ and 3’ homology arms were PCR-amplified using pDEST-APIGH (Addgene # 112804) as the template ...