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Citations for Addgene :
1 - 50 of 908 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: Bacterial stabs containing Sortase 5M were obtained from Addgene (#51140), deposited by the Ploegh lab.90 Individual colonies were picked from plated stabs for 10mL plasmid preps (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... a gfp PCR product was PCR amplified from pPD95.75 (Addgene) using primers containing 35bp of overlap with the spop-1 gene immediately upstream of the predicted nuclear localization sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into pCR Zero vector (#120275, Addgene) digested with StuI (R0187S ...
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Independent representations of reward-predicting cues and reward history in frontal cortical neuronsbioRxiv - Neuroscience 2020Quote: ... The AAV solution (AAV1-hSyn-NES-jRGECO1a, titer ~1 × 1013 GC/ml, the viral solution provided by Addgene, viral prep # 100854-AAV1 ...
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bioRxiv - Neuroscience 2023Quote: ... Viral solutions (AAV9-CaMKII-somBiPOLES-mCerulean, Addgene) were loaded into beveled glass injection needles (WPI ...
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bioRxiv - Immunology 2022Quote: ... annealed oligos were diluted 1:20 in molecular grade H2O and then ligated into Esp3I-digested pLentiCRISPR V2.0 (Addgene #52961). Ligation reactions were then transformed into Top10 competent E ...
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bioRxiv - Immunology 2022Quote: ... PCR amplified HGS or VPS37A from U937 cDNA and PCR amplified mScarlet from pmScarlet_C1 (Addgene #85042) into pTRIP-SFFV-EGFP-NLS (Addgene #86677) ...
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bioRxiv - Molecular Biology 2022Quote: PCR fragment with pY010 (Addgene #69982) as template and the following primers was cloned into pX458 (Addgene # 48138 ...
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bioRxiv - Genetics 2022Quote: ... which we PCR amplified from Addgene plasmid #67639 ...
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bioRxiv - Genetics 2022Quote: ... we PCR amplified NatMX from Addgene plasmid #35121 with the primers listed in Supplementary Table 6 using Phusion Hot Start Flex DNA polymerase (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... emiRFP670 were PCR amplified from Addgene Plasmid #136571 (Matlashov et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and pCR-24xPP7SL (Addgene, Cat.No. 31864) plasmids were double digested using SpeI and NotI restriction enzymes and the 24xMS2SL (1352bp ...
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bioRxiv - Neuroscience 2022Quote: ... a virus solution (rAAV1-hSyn-jGCamP7f, 104488-AAV1, Addgene) was injected under stereotaxic conditions (400 nL ...
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bioRxiv - Immunology 2023Quote: ... The wzm – wbbO PCR product was assembled with PCR-linearized pBBR1MCS2 plasmid (Addgene, primers pBBR1-1F/pBBR1-1R) using an NEBuilder HiFi DNA Assembly kit (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... was PCR-amplified from plasmid pKIR1.1 (Addgene). Venus codon-optimized for expression in C ...
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bioRxiv - Immunology 2020Quote: ... we PCR amplified TagRFP-Flag-cGAS (Addgene) by using RFP-cGAS-XbaI-F and cGAS-XhoI-R primers (Table S1) ...
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bioRxiv - Biophysics 2023Quote: FUS was PCR-amplified from pDEST8_FLAGHA_FUS_HIS (Addgene plasmid no ...
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bioRxiv - Cancer Biology 2023Quote: ... YAP5SA was amplified by PCR from Addgene #33093 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human Sin1.1 was PCR-amplified from Addgene 73388 plasmid (gift from Taekjip Ha32 ...
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bioRxiv - Immunology 2024Quote: ... STAT1 cDNA was PCR-amplified from Addgene #8691 (a gift from Jim Darnell (Horvath et al ...
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bioRxiv - Synthetic Biology 2024Quote: ... or PCR amplified via plasmids from Addgene, by primers ordered from IDT and Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The second solution contained 150 ng pCMV-VSVG (Addgene 8454), 400 ng psPAX2 (Addgene 12260) ...
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bioRxiv - Genomics 2021Quote: ... a stock solution of AAV8-TBG-Cre (Addgene 107787-AAV8) was diluted in PBS to a total volume of 20 μL and injected intraperitoneally into postnatal day five mice ...
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bioRxiv - Neuroscience 2020Quote: ... we intraventricularly injected a viral solution (pAAV.Syn.GCaMP6s.WPRE.SV40, Addgene #100843-AAV1) at P0 in mouse pups (Figure 1A-B) ...
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bioRxiv - Developmental Biology 2023Quote: ... A second solution contained 10.2 μg pMD2.G (Addgene, #12259), 15.6 μg psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... Rtn4b-HaloTag was generated by inserting PCR-amplified Rtn4b from Rtn4b-GFP and PCR-amplified HaloTag from pSEMS-Tom20-HaloTag (Addgene plasmid #111135 ...
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bioRxiv - Molecular Biology 2022Quote: The Dux promoter was amplified by PCR with PCR mix (Yeasen, 10149ES03) and then cloned into pGL4.10[luc2] (Addgene, E6651) vector ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments amplified from pFLAG-attP (Addgene, #110095) with primers Apr fw/Apr rv and from pSEVA524 with primers tetA fw/tetA rv,25 respectively ...
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bioRxiv - Genetics 2022Quote: ... The TDH3 promoter was PCR-amplified from Addgene plasmid #67639 (a gift from John Wyrick) ...
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bioRxiv - Genetics 2022Quote: ... We PCR amplified the NatMX cassette from Addgene plasmid #35121 using primers with homology to the 5’ upstream and 3’ downstream sequences of the targeted gene ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product and pCS2-FLAG (AddGene 16331) were digested with EcoRI and XhoI and ligated with T4 DNA Ligase ...
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bioRxiv - Cell Biology 2022Quote: ... A PCR product of ColX (from Addgene #110726) and a synthetic gene containing PAUF (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were cloned into pRAV23 (Addgene) using EcoRI and HindIII restriction sites and transformed into Top10 cells ...
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bioRxiv - Cell Biology 2020Quote: ... a PCR product of myc-BirA* (35700; Addgene) was ligated into XhoI and SpeI cleaved pCAG-mNCad-HA ...
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bioRxiv - Cancer Biology 2023Quote: ... HOXB13 was PCR amplified from pLX302_HOXB13 (Addgene #70089), digested with SalI/XbaI and ligated into linearized pENTR1A (Addgene #17398) ...
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bioRxiv - Developmental Biology 2024Quote: ... was PCR amplified from pJW2171 (Addgene plasmid #163095) and concentrated using a PCR purification kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... which was PCR-amplified from pSFGFP-N1 (Addgene) [55].
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bioRxiv - Cell Biology 2022Quote: ... The iRFP ORF was PCR amplified from Addgene plasmid #45457.
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bioRxiv - Neuroscience 2024Quote: ... Gamillus obtained by PCR reaction from (Addgene #124837) was inserted in place of SEP with a use of NEBuilder HiFi DNA Assembly ...
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bioRxiv - Molecular Biology 2024Quote: ... with DNA sequences amplified by PCR from Addgene plasmids (102881 ...
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bioRxiv - Genetics 2021Quote: ... DNA templates for PCR-IVT were produced using overlapping oligonucleotides in a high-fidelity PCR reaction47 or using a plasmid template (Addgene #4223048) and appropriate primers46 ...
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was used as a template for PCR and 2500ng of purified HDR template PCR product combined with 1500ng of guide RNA expression plasmid (Addgene 49330) containing the guide RNA listed in Table S4 were co-transfected into OSCs ...
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bioRxiv - Cancer Biology 2020Quote: ... which was PCR-amplified from pEMS1384 (Addgene Plasmid #29304). The pHes5-d2EGFP plasmid was a gift from Ryoichiro Kageyama (Ohtsuka et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplified and cloned into lentiCRISPRv2 (Addgene Plasmid #52961) and lentiGUIDE-puro (Addgene Plasmid #52963 ...
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bioRxiv - Neuroscience 2020Quote: ... we first PCR amplified the OXTR sequence (Addgene, #66467) to contain ClaI restriction enzyme sites at each end ...
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bioRxiv - Neuroscience 2020Quote: ... the human ACE2 ORF was PCR amplified from Addgene plasmid 1786 and C-terminally fused with the porcine teschovirus-1-derived P2A cleavage sequence (ATNFSLLKQAGDVEENPGP ...
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bioRxiv - Biophysics 2020Quote: Full length HP1α was amplified by PCR (Addgene 17652), and cloned using InFusion kit into a pHR lentiviral vector under an SFFV promoter and tagged C-terminally with mCherry and sspB ...
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bioRxiv - Cell Biology 2022Quote: ... mApple and sfCherry2 cDNAs were PCR-amplified from Addgene plasmids #54862 and #83031 ...
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bioRxiv - Molecular Biology 2022Quote: ... one obtained by PCR on pRPR1_gRNA_handle_RPR1t (Addgene Plasmid #49014) using OFS_2869 and OFS_2870 oligonucleotides ...
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bioRxiv - Neuroscience 2020Quote: ... Postsynaptic mouse neuroligin-1 was PCR amplified from Addgene #15260 ...