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Citations for Addgene :
51 - 100 of 908 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: A PCR product from plasmid pDD282 (Addgene plasmid # 66823) was used as a donor template for insertion of gfp ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were subcloned into the pUC19 plasmid (Addgene) and sequenced.
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bioRxiv - Molecular Biology 2022Quote: ... PUM1 was PCR amplified from pMAL-PUM1 (Addgene #120384) and PUM2 was PCR amplified from pMAL-PUM2 (Addgene #120385) ...
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bioRxiv - Microbiology 2020Quote: ... the human hACE2 ORF was PCR amplified from Addgene plasmid 1786 and C-terminally fused with the porcine teschovirus-1-derived P2A cleavage sequence (ATNFSLLKQAGDVEENPGP ...
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bioRxiv - Neuroscience 2020Quote: ... we PCR-amplified the CEPIA coding region from Addgene plasmids pCMV CEPIA3mt and pCMV CEPIA4mt (Suzuki et al ...
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bioRxiv - Genomics 2022Quote: ... The PCR primers and conditions are available from Addgene. The PCR product were mixed and cleaned using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... KAP1 was PCR amplified from pEGFP-KAP1 (Addgene, #45568) and inserted in place of PMLIII into pBOS-PMLIII-YFP plasmid.
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bioRxiv - Systems Biology 2024Quote: ... PpHIS4 gene was PCR amplified from pMJA089 (Addgene #128518) (Yang et al. ...
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bioRxiv - Genetics 2022Quote: ... Gata6 cDNA was PCR amplified from pSAM2_mCherry_Gata6 (Addgene #72694) and ligated using InFusion cloning.
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bioRxiv - Synthetic Biology 2024Quote: ... The miR-30a scaffold was PCR amplified from Addgene vector #25748 ...
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bioRxiv - Immunology 2024Quote: ... and GFP PCR amplification from plasmid pDSAG (Addgene #62289) respectively ...
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bioRxiv - Neuroscience 2021Quote: ... The virus solution (1000 nl, titer ≥1013 vg/ml, #100836-AAV9 from Addgene) was injected in the SCN (ML +/-0.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... crassa genomic DNA with primers 598 and 599 by PCR and inserting the PCR product into the SpeI site of Plasmid 43802 (Addgene, DiCarlo et al. 2013). The resulting plasmid was used as the template for PCR-based amplification of tef-1(P)-cas9Hs with primers 610 and 589 ...
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bioRxiv - Cell Biology 2020Quote: ... We PCR amplified d2EGFP from M38 TOP-dGFP (Addgene #17114), engineering XhoI and NotI sites using primer sequences 5’-AAACTCGAG-GCCACCATGGTGAGCAAGG and 5’-AAAGCGGC-CGCCTACACATTGATCCTAGCAGAAG and cloned the coding region upstream of the β-globin-UTR ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR product was then subcloned into pcDNA3.1+ (Addgene) using BamHI and NotI restriction endonucleases (pcDNA3.1+-KanSacB).
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bioRxiv - Developmental Biology 2021Quote: Kaede PCR product was amplified from the plasmid (Addgene #54726) for generating the template for capped mRNA synthesis (primers F ...
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bioRxiv - Microbiology 2021Quote: ... were PCR amplified and inserted into the plasmid pFGL1010 (Addgene, 119081 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR amplified and cloned into lentiGuide-Puro vector (Addgene #52963). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... the gene encoding mRFPmars was PCR amplified from pmRFPmars (Addgene) with primers 4572/4573 ...
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bioRxiv - Cell Biology 2022Quote: Mouse Drp1 was PCR amplified from pcDNA3.1 mDrp1 (Addgene #34706) and cloned into pLenti BlastR using InFusion cloning ...
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bioRxiv - Microbiology 2021Quote: ... the gene encoding mRFPmars was PCR amplified from pmRFPmars (Addgene) with primers 4572/4573 ...
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bioRxiv - Genomics 2021Quote: ... KRAB and dCas9 were PCR amplified from pCC_09 (Addgene 139094) (69 ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was placed in pME (pDONR221 #398, Addgene) using a Gateway recombination reaction with BP clonase II ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP was amplified by PCR from pLeGO-iG2 (Addgene #27341). The IRES site was amplified by PCR from pInducer-21 (Addgene #46948) ...
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bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of IKK2-EE (Addgene, 11105) with BclI–MfeI ends and inserting into pBABE puro (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... PCR amplified constructs were inserted into mCherry-CRY2clust (Addgene #105624), using BsrGI/HpaI sites to obtain mCherry tagged JNK2α2 and SgrAI/HpaI sites to obtain mCherry tagged ASK1 constructs ...
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bioRxiv - Cell Biology 2023Quote: ... EBFP2 amplified by PCR from mTagBFP2-C1 plasmid (Addgene #54665), BbsI-silenced Sniper-Cas9 amplified by PCR ...
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bioRxiv - Cell Biology 2023Quote: ... NEMOD311N was obtained by PCR of pGEX- NEMOD311N (Addgene #11968). GFP was obtained by PCR of a GFP-containing plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and PUM2 was PCR amplified from pMAL-PUM2 (Addgene #120385). For PUM1 plasmids ...
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bioRxiv - Cancer Biology 2022Quote: CreERt2 was amplified by PCR using pCGA_creERt2 (Addgene plasmid #14797) as template ...
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bioRxiv - Cell Biology 2022Quote: ... jGCaMP7s was PCR amplified from pGP-CMV-jGCaMP7s (Addgene #104463). Following gel extraction ...
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bioRxiv - Cell Biology 2022Quote: ... sgDNA was first PCR-amplified from pDD162 vector (Addgene 47549) using primers targeting the SWIP sequence ...
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bioRxiv - Physiology 2022Quote: ... TBG promoter was PCR-cloned from the pAAV.TBG.PI.eGFP.WPRE.bGH (Addgene #105535) using primers with XbaI cloning site (5’-GGTTCTAGATGCATGTATAATTTCTACAG ...
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bioRxiv - Microbiology 2022Quote: ... cpGFP was PCR-amplified from pTKEI-Tre-C04 34 (Addgene plasmid #79754 ...
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bioRxiv - Neuroscience 2022Quote: ... we first PCR amplified mGreenLantern from pcDNA3.1-mGreenLantern (Addgene #161912). Using MluI and XbaI ...
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bioRxiv - Developmental Biology 2023Quote: ... an amplified PCR product from pCAG-TAG (Addgene, Plasmid #26771) was cloned into the KpnI-NotI sites of pcDNA3.1-H2B mCherry-poly(A83 ...
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bioRxiv - Microbiology 2024Quote: ... was constructed by PCR amplification of pCas1+214 (Addgene #72676) to replace the T7 promoter by an IPTG-inducible Lac promoter.
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bioRxiv - Neuroscience 2021Quote: ... We first unilaterally injected a cocktail solution of pENN.AAV.CaMKII.0.4.Cre.SV40 (Addgene#105558-AAV9) and AAV2/rh8-synP-DIO-sTpEpB-WPRE-bGH with a mixed ration of 1:1 at M2 (1 μl ...
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bioRxiv - Neuroscience 2024Quote: ... An adeno-associated virus (AAV) solution expressing jGCaMP7s under the human synapsin promoter (Addgene catalog number 104487-AAV1 ...
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bioRxiv - Molecular Biology 2020Quote: ... were PCR amplified from pcDNA4/TO-ORF24-3xFLAG (19) (Addgene #138423) with primers to introduce BamHI and NotI sites and cloned into pcDNA4/TO-3xFLAG (C-terminal tag ...
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bioRxiv - Molecular Biology 2021Quote: SARS-CoV-2 viral proteins were amplified via PCR from Addgene constructs with a 1x (GGGS ...
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bioRxiv - Genetics 2020Quote: ... Gal80 coding sequence was PCR amplified from pBPGAL80Uw-4 (Addgene 26235). A His2Av polyA sequence after the BFP/Gal80 coding sequence was PCR amplified from pDEST-HemmarR2 (Addgene # 112813).
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bioRxiv - Immunology 2021Quote: ... Ovalbumin (OVA) amplicon was PCR amplified from pcDNA3-OVA (Addgene #64599) and cloned into pHIV-Luc-ZsGreen backbone (Addgene #39196) ...
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bioRxiv - Cancer Biology 2021Quote: ... Positive controls for PCR used lentiguide-Puro EGFP gRNA (Addgene 80036) which contained a gRNA sequence not present in the GeCKO library ...
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bioRxiv - Cell Biology 2021Quote: ... Smo-GCaMP5G-mCherry was generated by PCR-amplification of GCaMP5G (Addgene plasmid 31788 ...
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bioRxiv - Cell Biology 2021Quote: ... mKeima was amplified by pcr using mKeima-RED-N1 (Addgene #54597) as a template ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR-amplified Cas9n-NG DNA from pX330-SpCas9-NG (Addgene# 117919), PCR-amplified UGI DNA from pLenti-TRE3G-FNLS-PGK-Puro (Addgene# 110847 ...
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bioRxiv - Cell Biology 2021Quote: ... which was subcloned into the pCR 2.1TOPO vector (AddGene, Cambridge, MA). The antisense digoxigenin-labeled hybridization probes were transcribed in vitro using the T7 MAXIscript kit ...
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bioRxiv - Microbiology 2021Quote: ... The FLT3 was PCR amplified from pAL119-FLT3L (Addgene, item #21910), and the TBG was amplified from pX602-AAV-TBG:NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... and a PCR amplicon containing the CRISPRoff-v2.1 protein from Addgene plasmid 167981 (gifted from Luke Gilbert [45] ...