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Citations for Addgene :
101 - 150 of 908 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: EGFP sequences were amplified by PCR from pGEM5Z(+)-EGFP (Addgene #65206) and were cloned into p(UASp ...
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bioRxiv - Genetics 2020Quote: ... Gal80 coding sequence was PCR amplified from pBPGAL80Uw-4 (Addgene 26235) using the oligonucleotides aaaaaaaaatcaaaATGAGCGGTACCGATTACAACAAAAGGAGTAGTGTGAG and GCCGACTGGCTTAGTTAattaattctagaTTAAAGCGAGTAGTGGGAGATGTTG ...
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bioRxiv - Neuroscience 2020Quote: ... Gephyrin sequences were amplified by PCR from pmCherryC2-Gephyrin P1 (Addgene) and subcloned into the pAAV-hSyn-mCherry (Addgene ...
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bioRxiv - Genetics 2020Quote: PE2 coding sequence was PCR amplified from pCMV-PE2 (Addgene # 132775) and gel purified ...
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bioRxiv - Genetics 2020Quote: PE2 coding sequence was PCR amplified from pCMV-PE2 (Addgene # 132775). pEntr backbone was PCR amplified from pEntr_D-TOPO (Invitrogen K240020) ...
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bioRxiv - Biochemistry 2021Quote: ... plasmids7 used in droplet digital PCR experiments were sourced from Addgene. The PSP1-targeting spacer was cloned into pHelper by Gibson assembly ...
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bioRxiv - Cancer Biology 2021Quote: ... mCherry was PCR amplified from pMSCV-Peredox-mCherry-NLS (Addgene, 32385) using primers containing overhangs with the homology sites for GMAP cloning ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR products were cloned into the pMAL-c2x (Addgene # 75286) vector ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were cloned into pNIC-CH (Addgene plasmid 26117) by ligation-independent cloning (42) ...
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bioRxiv - Cell Biology 2022Quote: ... and Eg5 were cloned using PCR into plasmid 462 (Addgene 89065) containing a Z3EV promoter (Ohira et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The ORF of EPLIN isoform alpha was PCR amplified from Addgene plasmid #40928 to generate plasmid #1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned the PCR products into overexpression vectors EGFP-N1 (Addgene) or pLenti6 (Addgene) ...
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bioRxiv - Systems Biology 2024Quote: ... was created by PCR cloning of 3xHA-MKK6-EE (Addgene, 47579) with BglII–SalI ends and inserting into pBABE puro (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... Firefly luciferase was PCR amplified from pCDNA-Luciferase (Addgene, cat # 18964) using primers introducing a 5’ KpnI site and a 3’ NheI site ...
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bioRxiv - Cancer Biology 2024Quote: ... HMGCR was PCR-amplified out of pCMV-SPORT6-hHMGCR1 (Addgene, 86085) using the forward primer 5’- GTATACTGGATCCGCCGCCACCATGTTGTCAAGACTT-3’ and reverse primer 5’-TCGGAGCTCGAGGTGGCTGTCTTCTTGGT-3’ ...
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bioRxiv - Immunology 2024Quote: ... mCherry was amplified by PCR from the mCherry-Rab4a (Addgene #55125) plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S ...
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bioRxiv - Systems Biology 2023Quote: ... and XRCC6 were cloned by PCR into pEN_TT_miRc2 3xFLAG (Addgene #83274)64 ...
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bioRxiv - Cell Biology 2023Quote: ... KRT5 cDNA was PCR amplified using pBabe mRFP1-KRT5 (Addgene#59493) as template using Phusion High Fidelity Polyemrase (F503 ...
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bioRxiv - Bioengineering 2023Quote: ... into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326) to replace the red fluorescent protein (RFP ...
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bioRxiv - Bioengineering 2023Quote: ... the Cas9 gene was PCR amplified from pwtCas9-bacteria (Addgene #44250) and a gBlock (purchased from Integrated DNA Technologies [IDT] ...
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bioRxiv - Genetics 2023Quote: ... the CHD7 ORF was PCR amplified from a plasmid (Addgene, 89460) using Phusion High-Fidelity Master Mix (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... the iTol2 flanked kanR-cryaa:sfGFP fragment was PCR amplified from Addgene #74153 (Fuentes et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplified 8xtetO-EF1a promoter-eGFP cassette from iDuet101a (Addgene, #17629) was cloned with Gibson assembly in frame with the SV40pA sequences that were PCR amplified from lentiCRIPSRv2 (Addgene ...
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bioRxiv - Genomics 2022Quote: ... and the previously digested hu6 PCR fragment (from pMJ117 Addgene 85997) with appropriate overhangs was inserted via T4 ligation ...
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bioRxiv - Synthetic Biology 2023Quote: ... The following expression vectors were amplified by PCR: RAD51 (Addgene #41815), CtIP (Addgene #109403) ...
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bioRxiv - Cell Biology 2023Quote: ... APEX2 was obtained by PCR from APEX2-csChBP (no. 108876, Addgene) and inserted in pMK-289 (no ...
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bioRxiv - Neuroscience 2023Quote: ... mScarlet was PCR amplified from the plasmid pmScarlet_C1 (Addgene plasmid #85042) while also adding a fyn myristolyation sequence (a membrane targeting sequence ...
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bioRxiv - Cell Biology 2023Quote: ... and mEmerald amplified by PCR from mEmerald-Sec61b-C1 (Addgene #90992), with a Gly-Gly-Gly-Ser linker placed either side of the HaloTag or mEmerald at Site 3 and Site 4 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was further cloned into pGEX-4T-1 (Addgene) in-frame with the N-terminal GST-tagged fusion construct(21) ...
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bioRxiv - Cell Biology 2024Quote: ... the CRY2 PHR domain was PCR amplified from pCRY2PHR-mCherryN1 (Addgene plasmid #26866 ...
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bioRxiv - Genetics 2024Quote: ... a 3XFLAG-NatMX cassette was PCR-amplified from pYC46 plasmid (Addgene) and inserted at the C-terminus of ZCF27 and ZCF4 (74 ...
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bioRxiv - Cancer Biology 2024Quote: ... Resulting PCR product was cut and ligated to pENTR1A (Addgene #17398) entry vector ...
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bioRxiv - Neuroscience 2024Quote: ... GCaMP6f gene was PCR amplified from pGP-CMV-GCaMP6f (Addgene #40755) with Q5 High-Fidelity 2X Master Mix (M0492S ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments were assembled into pLenti6.3/V5- DEST-GFP (Addgene #40125) by replacing the CMV promoter and EGFP sequences at the 5’ ClaI and 3’ SalI restriction sites ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR amplicons were cloned into the pAGM9121 (Addgene plasmid #51833), pICH41308 (Addgene plasmid #47998 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product were then subcloned into pCaSpeR4 transformation vector (Addgene). Transgenic fly lines were generated by a standard procedure with P-element mediated transformation of w1118 embryos (Rainbow transgenic Flies Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... PCR product and recipient backbone (pInducer10b-HA-KRas G12V Addgene #164928) were digested with AgeI-HF and MluI-HF for 1h at 37°C ...
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bioRxiv - Genetics 2024Quote: ... and Snap sequence was PCR amplified from pAAV-myrSNAP (Addgene, #60098). Fragments were inserted into pAAV using T4 ligase (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... 0.8 μl of AAV solution containing pZac2.1 gfaABC1D-cyto-GCaMP6f (Addgene viral prep # 52925-AAV5) was injected at 100 nL/min by means of a hydraulic injection apparatus driven by a syringe pump (UltraMicroPump ...
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bioRxiv - Neuroscience 2023Quote: ... 0.4 μl of AAV solution containing pZac2.1 gfaABC1D-cyto-GCaMP6f (Addgene viral prep # 52925-AAV5) was injected at 50 nL/min using a hydraulic injection apparatus driven by a syringe pump (UltraMicroPump ...
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bioRxiv - Neuroscience 2024Quote: ... we injected a solution of 1μg/μl containing the plasmid pCAG-GFP (Addgene plasmids #11150) into the embryo’s lateral ventricle using a 30 μm pulled glass micropipette ...
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bioRxiv - Cancer Biology 2021Quote: ... The PCR product was cloned into the pENTR4 no ccDB plasmid (Addgene #17424 ...
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bioRxiv - Cell Biology 2020Quote: ... full length P180 was PCR amplified from V5-GFP-P180 (Addgene #92150) and inserted in mCherry-N1 vector between XhoI and BamHI sites ...
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bioRxiv - Developmental Biology 2021Quote: ... the 24xMS2 stem loop sequence was purified from pCR-24xMS2L-stable (Addgene_31865) by restriction digest and the 128xMS2 from pMK123-128xMS2 (Tantale et al. ...
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bioRxiv - Genetics 2020Quote: The tdTomato coding sequence was PCR-amplified from pDEST-HemmarR2 (Addgene # 112813) and cloned into EcoRI/XbaI sites of pUA (Addgene 58372).
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bioRxiv - Genetics 2020Quote: ... Cas9 coding sequence was PCR-amplified using pEDST-APIC-Cas9 (Addgene # 121657) as the template ...
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bioRxiv - Bioengineering 2021Quote: ... fragments were PCR-amplified and cloned into the px552 plasmid (Addgene #60958) to make px552-U6-CAG-mCherry ...
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bioRxiv - Immunology 2021Quote: ... BRD9-dBD and BRD9-N216A were PCR-amplified from existing vectors (Addgene plasmids #6537968 and #75114-648 ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting PCR product was inserted into pLVpuro-CMV-N-EGFP (Addgene plasmid # 122848 ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments of CgB (DNASU) and CLIP (gift from Eric Campeau; RRID:Addgene_29650) were subcloned into pENTR-RIP via Gibson assembly ...